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001-es BibID:	BIBFORM039260
Első szerző:	Holub, B. S.
Cím:	The neuropeptide galanin is a novel inhibitor of human hair growth / Holub B. S., Kloepper J. E., Tóth B. I., Bíro T., Kofler B., Paus R.
Dátum:	2012
ISSN:	0007-0963
Megjegyzések:	BACKGROUND: Galanin is a trophic factor of the central and peripheral nervous system that shows widespread distribution in human skin. However, the exact localization and the role of galanin in the hair follicle (HF) remain to be clarified. OBJECTIVES: To characterize galanin expression in human scalp HFs and to examine the effects of galanin on normal human scalp HF growth in organ culture. METHODS: Immunohistochemistry was performed on cryosections of human female scalp skin. Anagen HFs were microdissected and cultured up to 9 days and treated with 100 nmol L(-1) galanin. Staining for Ki-67, TUNEL and Masson-Fontana were used to analyse proliferation, apoptosis and hair cycle staging of the HFs. Functional effects of galanin were tested in serum-free HF organ culture. RESULTS: Galanin-like immunoreactivity was detected in the outer root sheath (ORS) and inner root sheath. Additionally, galanin mRNA was detected in ORS keratinocytes and all HF samples tested. Galanin receptor transcripts (GalR2, GalR3) were also detected in selected samples. Galanin reduced proliferation of hair matrix keratinocytes in situ compared with vehicle-treated controls, shortened the hair growth phase (anagen) in vitro and reduced hair shaft elongation. This was accompanied by the premature development of a catagen-like morphology of galanin-treated HFs. CONCLUSIONS: We present the first evidence that human HFs are both a source and a functionally relevant target of galanin. Due to its hair growth-inhibitory properties in vitro, galanin application deserves further exploration as a potential new treatment strategy for unwanted hair growth (hirsutism, hypertrichosis).
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	British Journal Of Dermatology. - 167 : 1 (2012), p. 10-16. -
További szerzők:	Kloepper, Jennifer E. Tóth István Balázs (1978-) (élettanász) Bíró Tamás (1968-) (élettanász) Kofler, Barbara Paus, Ralf
Pályázati támogatás:	Austrian Research Promotion Agency FFG Egyéb 822782/THERAPEP Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM048833
035-os BibID:	PMID:23949722
Első szerző:	Vidali, Silvia
Cím:	Hypothalamic-Pituitary-Thyroid Axis Hormones Stimulate Mitochondrial Function and Biogenesis in Human Hair Follicles / Silvia Vidali, Jana Knuever, Johannes Lerchner, Melanie Giesen, Tamás Bíró, Matthias Klinger, Barbara Kofler, Wolfgang Funk, Burkhard Poeggeler, Ralf Paus
Dátum:	2014
ISSN:	0022-202X
Megjegyzések:	Thyroid hormones regulate mitochondrial function. As other hypothalamic-pituitary-thyroid (HPT) axis hormones, i.e., thyrotropin-releasing hormone (TRH) and thyrotropin (TSH), are expressed in human hair follicles (HFs) and regulate mitochondrial function in human epidermis, we investigated in organ-cultured human scalp HFs whether TRH (30?nM), TSH (10?mU?ml-1), thyroxine (T4) (100?nM), and triiodothyronine (T3) (100?pM) alter intrafollicular mitochondrial energy metabolism. All HPT-axis members increased gene and protein expression of mitochondrial-encoded subunit 1 of cytochrome c oxidase (MTCO1), a subunit of respiratory chain complex IV, mitochondrial transcription factor A (TFAM), and Porin. All hormones also stimulated intrafollicular complex I/IV activity and mitochondrial biogenesis. The TSH effects on MTCO1, TFAM, and porin could be abolished by K1-70, a TSH-receptor antagonist, suggesting a TSH receptor-mediated action. Notably, as measured by calorimetry, T3 and TSH increased follicular heat production, whereas T3/T4 and TRH stimulated ATP production in cultured HF keratinocytes. HPT-axis hormones did not increase reactive oxygen species (ROS) production. Rather, T3 and T4 reduced ROS formation, and all tested HPT-axis hormones increased the transcription of ROS scavengers (catalase, superoxide dismutase 2) in HF keratinocytes. Thus, mitochondrial biology, energy metabolism, and redox state of human HFs are subject to profound (neuro-)endocrine regulation by HPT-axis hormones. The neuroendocrine control of mitochondrial biology in a complex human mini-organ revealed here may be therapeutically exploitable.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Calcium (Ca2+) signalling Ion channel Membrane transport Molecular mechanisms of disease Skin TRP channels
Megjelenés:	Journal of Investigative Dermatology. - 134 (2014), p. 33-42. -
További szerzők:	Knüver, Jana Lerchner, Johannes Giesen, Melanie Bíró Tamás (1968-) (élettanász) Klinger, Matthias Kofler, Barbara Funk, Wolfgang Poeggeler, Burkhard Paus, Ralf
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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