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001-es BibID:BIBFORM049397
Első szerző:Szegedi Csaba
Cím:Effect of maurocalcine on the calcium release channel / Csaba Szegedi, János Almássy, Jan-Marc Sabatier, István Jóna
Dátum:2004
ISSN:0006-3495
Megjegyzések:The toxin Maurocalcine (MCa) isolated from scorpion Scorpio maurus palmatus contains 33 amino acids and three important disulphide bridges. This compound was chemically synthesized and its effect studied using purified RyR1 incorporated into bilayer under voltage clamp condition. Application of MCa on the cis side of the bilayer induces characteristic long lasting subconductance state (LLSSs), representing 60% of the full conductance. The channel spend 54.8% of the time in this LLSSs at 472 nM calcium using 50 nM of MCa. Duration distribution analysis reveals one single exponential component, showing 363?42 ms mean LLSS duration. Exchanging AA24 of MCa for alanine result in the loss of LLSSs. Exchanging AA23 of MCa for alanine result a decrease of the overall LLSS effect by several fold influencing the frequency and the duration of the LLSS in different way. The frequency and the duration of LLSS induced by wtMCa is polarity dependent, however still ohmic and the "inter LLSS" open probability is increased tenfold at 200 nM MCa concentrations. The first order concentration dependence of the mean duration of the LLSSs suggests one effective binding site per functional channel. Interestingly, exchanging AA22 of MCa for alanine result a decrease of the overall LLSS effect resulting about 686?31 ms mean duration at 200 nM 22MCa concentrations, accompanied by substantially lower frequency. The MCa treated RyR1 still sensitive for ryanodine showing three conductance levels corresponding to the closed, ryanodine effected and ryanodine + MCa affected states. Nor the wild type, nor the mutants altered the SERCA1 ATPase activity. Supported by EU (HPRN-CT-2002-00331) and by OTKA (037727).
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
calcium release
Megjelenés:Biophysical Journal. - 84 : Suppl. 2 (2004), p. 224a. -
További szerzők:Almássy János (1981-) (élettanász, biológus, angol-magyar szakfordító) Sabatier, Jean Marc Jóna István (1948-) (élettanász, fizikus)
Pályázati támogatás:T037727
OTKA
Internet cím:Szerző által megadott URL
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