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001-es BibID:BIBFORM070542
Első szerző:Bardóczi Zsuzsanna
Cím:Glycinergic input to the mouse basal forebrain cholinergic neurons / Bardóczi Z., Pál B., Kőszeghy Á., Wilheim T., Watanabe M., Záborszky L., Liposits Z., Kalló I.
Dátum:2017
Megjegyzések:The basal forebrain (BF) receives afferents from brain stem ascending pathways, which has been implicated first by Moruzzi and Magoun (Moruzzi and Magoun, 1949) to induce forebrain activation and cortical arousal/waking behavior; however, it is very little known about how brain stem inhibitory inputs affect cholinergic functions. In the current study, glycine, a major inhibitory neurotransmitter of brain stem neurons, and gliotransmitter of local glial cells, was tested for potential interaction with basal forebrain cholinergic (BFC) neurons in male mice. In the BF, glycine receptor ? subunit-immunoreactive (GlyR?-IR) sites were localized in choline acetyltransferase (ChAT)-IR neurons. Glycine's effect on BFC neurons was demonstrated by bicuculline-resistant, strychnine-sensitive spontaneous inhibitory postsynaptic currents (IPSCs; 0.81 ? 0.25 *10-1 Hz) recorded in whole cell conditions. Potential neuronal, as well as glial sources of glycine were indicated in the extracellular space of cholinergic neurons by glycine transporter 1 and 2 (GLYT1 and 2)-IR processes found in apposition to ChAT-IR cells. Ultrastructural analyses identified synapses of GLYT2-positive axon terminals on ChAT-IR neurons, as well as GLYT1-positive astroglial processes, which were localized in the vicinity of synapses of ChAT-IR neurons. The brain stem raphe magnus was determined to be a major source of glycinergic axons traced retrogradely from the BF. Our results indicate a direct effect of glycine on BFC neurons. Furthermore, the presence of high levels of plasma membrane glycine transporters in the vicinity of cholinergic neurons suggests a tight control of extracellular glycine in the BF.SIGNIFICANCE STATEMENTBFC neurons receive various activating inputs from specific brain stem areas, and channel this information to the cortex via multiple projections. So far very little is known about inhibitory brain stem afferents to the BF. The current study established glycine as a major regulator of BFC neurons by (1) identifying glycinergic neurons in the brain stem projecting to the BF, (2) showing GlyR?-IR sites in ChAT-IR neurons, (3) demonstrating GLYT2-positive axon terminals synapsing on ChAT-IR neurons, and (4) localizing GLYT1-positive astroglial processes in the vicinity of synapses of ChAT-IR neurons. Glycine's effect on BFC neurons was demonstrated by bicuculline-resistant, strychnine-sensitive spontaneous IPSCs recorded in whole cell conditions.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
basal forebrain
glycinergic
Megjelenés:The Journal of Neuroscience 37 : 39 (2017), p. 9534-9549. -
További szerzők:Pál Balázs (1975-) (élettanász) Kőszeghy Áron (1983-) (Ph.D hallgató, élettanász) Wilheim Tamás Watanabe, Masahiko Záborszky László Liposits Zsolt Kalló Imre
Pályázati támogatás:KTIA_13_NAP-A-I/10
Egyéb
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2.

001-es BibID:BIBFORM018456
Első szerző:Elfant, David
Cím:Specific inhibitory synapses shift the balance from feedforward to feedback inhibition of hippocampal CA1 pyramidal cells / Elfant D., Pál B. Z., Emptage N., Capogna M.
Dátum:2008
ISSN:0953-816X
Megjegyzések:Feedforward and feedback inhibition are two fundamental modes of operation widespread in the nervous system. We have functionally identified synaptic connections between rat CA1 hippocampal interneurons of the stratum oriens (SO) and interneurons of the stratum lacunosum moleculare (SLM), which can act as feedback and feedforward interneurons, respectively. The unitary inhibitory postsynaptic currents (uIPSCs) detected with K-gluconate-based patch solution at -50 mV had an amplitude of 20.0 +/- 2.0 pA, rise time 2.2 +/- 0.2 ms, decay time 25 +/- 2.2 ms, jitter 1.4 +/- 0.2 ms (average +/- SEM, n = 39), and were abolished by the gamma-aminobutyric acid (GABA)(A) receptor antagonist 2-(3-carboxypropyl)-3-amino-6-methoxyphenyl-pyridazinium bromide (SR 95531). Post hoc anatomical characterization revealed that all but one of the identified presynaptic neurons were oriens-lacunosum moleculare (O-LM) cells, whereas the postsynaptic neurons were highly heterogeneous, including neurogliaform (n = 4), basket (n = 4), Schaffer collateral-associated (n = 10) and perforant path-associated (n = 9) cells. We investigated the short-term plasticity expressed at these synapses, and found that stimulation at 10-40 Hz resulted in short-term depression of uIPSCs. This short-term plasticity was determined by presynaptic factors and was not target-cell specific. We found that the feedforward inhibition elicited by the direct cortical input including the perforant path onto CA1 pyramidal cells was modulated through the inhibitory synapses we have characterized. Our data show that the inhibitory synapses between interneurons of the SO and SLM shift the balance between feedback and feedforward inhibition onto CA1 pyramidal neurons.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
hippocampal neurons
interneuron
paired recording
rat
synaptic transmission
Megjelenés:European Journal Of Neuroscience. - 27 : 1 (2008), p. 104-113. -
További szerzők:Pál Balázs (1975-) (élettanász) Emptage, Nigel Capogna, Marco
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3.

001-es BibID:BIBFORM009112
Első szerző:Pál Balázs (élettanász)
Cím:Targets, receptors and effects of muscarinic neuromodulation on giant neurones of the rat dorsal cochlear nucleus / Pal, B., Koszeghy, A., Pap, P., Bakondi, G., Pocsai, K., Szucs, G., Rusznak, Z.
Dátum:2009
ISSN:0953-816X
Megjegyzések:Although cholinergic modulation of the cochlear nucleus (CN) is functionally important, neither its cellular consequences nor the types of receptors conveying it are precisely known. The aim of this work was to characterise the cholinergic effects on giant cells of the CN, using electrophysiology and quantitative polymerase chain reaction. Application of the cholinergic agonist carbachol increased the spontaneous activity of the giant cells; which was partly the consequence of the reduction in a K(+) conductance. This effect was mediated via M4 and M3 receptors. Cholinergic modulation also affected the synaptic transmission targeting the giant cells. Excitatory synaptic currents evoked by the stimulation of the superficial and deep regions of the CN were sensitive to cholinergic modulation: the amplitude of the first postsynaptic current was reduced, and the short-term depression was also altered. These changes were mediated via M3 receptors alone and via the combination of M4, M2 and M3 receptors, when the superficial and deep layers, respectively, were activated. Inhibitory synaptic currents evoked from the superficial layer showed short-term depression, but they were unaffected by carbachol. In contrast, inhibitory currents triggered by the activation of the deep parts exhibited no significant short-term depression, but they were highly sensitive to cholinergic activation, which was mediated via M3 receptors. Our results indicate that pre- and postsynaptic muscarinic receptors mediate cholinergic modulation on giant cells. The present findings shed light on the cellular mechanisms of a tonic cholinergic modulation in the CN, which may become particularly important in evoking contralateral excitatory responses under certain pathological conditions
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
agonist
Carbachol
CN
Cochlear Nucleus
dorsal cochlear nucleus
Electrophysiology
giant
Health
neurone
physiology
Polymerase Chain Reaction
rat
receptor
Synaptic Transmission
Megjelenés:The European Journal of Neuroscience. - 30 : 5 (2009), p. 769-782. -
További szerzők:Kőszeghy Áron (1983-) (Ph.D hallgató, élettanász) Pap Pál (1981-) (élettanász) Bakondi Gábor (1980-) (élettanász) Pocsai Krisztina (1978-) (élettanász) Szűcs Géza (1948-) (élettanász) Rusznák Zoltán (1965-) (élettanász)
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4.

001-es BibID:BIBFORM040376
Első szerző:Rusznák Zoltán (élettanász)
Cím:The hyperpolarization-activated non-specific cation current (Ih) adjusts the membrane properties, excitability, and activity pattern of the giant cells in the rat dorsal cochlear nucleus / Rusznák Zoltán, Pál Balázs, Kőszeghy Áron, Fu YuHong, Szücs Géza, Paxinos George
Dátum:2013
ISSN:0953-816X
Megjegyzések:Giant cells of the cochlear nucleus are thought to integrate multimodal sensory inputs and participate in monaural sound source localization. Our aim was to explore the significance of a hyperpolarization-activated current in determining the activity of giant neurones in slices prepared from 10 to 14-day-old rats. When subjected to hyperpolarizing stimuli, giant cells produced a 4-(N-ethyl-N-phenylamino)-1,2-dimethyl-6-(methylamino) pyridinium chloride (ZD7288)-sensitive inward current with a reversal potential and half-activation voltage of ?36 and ?88 mV, respectively. Consequently, the current was identified as the hyperpolarization-activated non-specific cationic current (Ih). At the resting membrane potential, 3.5% of the maximum Ih conductance was available. Immunohistochemistry experiments suggested that hyperpolarization-activated, cyclic nucleotide-gated, cation non-selective (HCN)1, HCN2, and HCN4 subunits contribute to the assembly of the functional channels. Inhibition of Ih hyperpolarized the membrane by 6 mV and impeded spontaneous firing. The frequencies of spontaneous inhibitory and excitatory postsynaptic currents reaching the giant cell bodies were reduced but no significant change was observed when evoked postsynaptic currents were recorded. Giant cells are affected by biphasic postsynaptic currents consisting of an excitatory and a subsequent inhibitory component. Inhibition of Ih reduced the frequency of these biphasic events by 65% and increased the decay time constants of the inhibitory component. We conclude that Ih adjusts the resting membrane potential, contributes to spontaneous action potential firing, and may participate in the dendritic integration of the synaptic inputs of the giant neurones. Because its amplitude was higher in young than in adult rats, Ih of the giant cells may be especially important during the postnatal maturation of the auditory system.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
developing auditory system
h-current
spontaneous activity
synaptic transmission
ZD7288
Megjelenés:European Journal Of Neuroscience. - 37 : 6 (2013), p. 876-890. -
További szerzők:Pál Balázs (1975-) (élettanász) Kőszeghy Áron (1983-) (Ph.D hallgató, élettanász) Fu, YuHong Szűcs Géza (1948-) (élettanász) Paxinos, George
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