CCL

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1.

001-es BibID:BIBFORM037815
Első szerző:Kőszeghy Áron (Ph.D hallgató, élettanász)
Cím:Purkinje-like cells of the rat cochlear nucleus : a combined functional and morphological study / Kőszeghy, Á., Pál, B., Pap, P., Pocsai, K., Nagy, Zs., Szücs, G., Rusznák, Z.
Dátum:2009
ISSN:0006-8993
Megjegyzések:Purkinje-like cells (PLCs) of the cochlear nucleus (CN) are strongly calbindin positive neurones with unknown function. In the present work functional and morphological methods have been employed to provide data about PLCs in general, and about their possible involvement in the synaptic organisation of the CN in particular. PLCs had slightly elongated soma, from which a complex dendritic arborisation extended with highly variable dimensions. On the basis of their morphology, three classes of PLCs were identified. Positively identified PLCs fired a train of action potentials on sustained depolarization. When hyperpolarizing stimuli were applied, the presence of a slowly activating, ZD7288-sensitive inward current was noted that corresponded to the h-current. PLCs received both excitatory and inhibitory synaptic inputs. Functional experiments revealed that 76% and 14% of the spontaneous inhibitory postsynaptic currents recorded from the cell bodies of the PLCs were mediated via glycinergic and GABAergic synapses, respectively. PLCs presented strong cerebellin1-like immunoreactivity, but its distribution differed from that seen in cerebellar Purkinje cells. Our results indicate that PLCs are parts of the synaptic circuitry of the CN, thus they may be actively involved in the processing and analysis of auditory information.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Brain Research. - 1297 (2009), p. 57-69. -
További szerzők:Pál Balázs (1975-) (élettanász) Pap Pál (1981-) (élettanász) Pocsai Krisztina (1978-) (élettanász) Nagy Zsuzsanna (1986-) (élettanász) Szűcs Géza (1948-) (élettanász) Rusznák Zoltán (1965-) (élettanász)
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM017183
Első szerző:Nagy Dénes (vegyész)
Cím:Cytoplasmic Ca2+ concentration changes evoked by muscarinic cholinergic stimulation in primary and metastatic melanoma cell lines / Nagy Dénes, Kosztka Lívia, Pap Pál, Nagy Zsuzsanna, Rusznák Zoltán, Csernoch László, Szűcs Géza
Dátum:2011
ISSN:0960-8931
Megjegyzések:Experiments were carried out to explore differences between cultured primary andmetastatic melanoma cell lines in their muscarinic acetylcholine receptor-mediatedintracellular Ca2+ signalization. The expression of type 1 and type 3 muscarinicreceptors was detected and compared at the protein level using bothimmunocytochemistry and semiquantitative Western blotting. The functionality of themuscarinic receptors was tested by applying carbamylcholine (1 mM) and recording theassociated increases in cytoplasmic [Ca2+] using Ca2+ imaging with the application ofthe Ca2+ indicator dye fluo-4. Our data indicate that the expression levels of thereceptor proteins were not significantly different in the metastatic (HT199, HT168-M1)and the primary (WM35) cell lines. Although Ca2+ transients were evoked in all threecell lines by carbamylcholine, the proportion of the carbamylcholine-positive cells wassmaller amongst the WM35 cells. The Ca2+ transients could be effectively blocked byatropine (0.1 mM). The time courses of the Ca2+ transients were highly variable, and insome instances they showed a late (plateau-like) component whose presence cruciallydepended on the influx of extracellular Ca2+. When the extracellular Ca2+ concentrationwas reduced, the duration of the CCh-evoked transients was considerably decreased; aphenomenon that was more pronounced in the metastatic cell lines. Although there areno fundamental differences in the muscarinic receptor-mediated Ca2+ signalization ofthe primary and metastatic cell lines, the quantitative differences revealed in the presentwork may partially explain the increased malignancy and migratory potential of themetastatic cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
muscarine
carbachol
melanoma
Megjelenés:Melanoma Research. - 21 : 1 (2011), p. 12-23. -
További szerzők:Kosztka Lívia (1981-) (élettanász) Pap Pál (1981-) (élettanász) Nagy Zsuzsanna (1986-) (élettanász) Rusznák Zoltán (1965-) (élettanász) Csernoch László (1961-) (élettanász) Szűcs Géza (1948-) (élettanász)
Pályázati támogatás:K 78398
OTKA
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM009112
Első szerző:Pál Balázs (élettanász)
Cím:Targets, receptors and effects of muscarinic neuromodulation on giant neurones of the rat dorsal cochlear nucleus / Pal, B., Koszeghy, A., Pap, P., Bakondi, G., Pocsai, K., Szucs, G., Rusznak, Z.
Dátum:2009
ISSN:0953-816X
Megjegyzések:Although cholinergic modulation of the cochlear nucleus (CN) is functionally important, neither its cellular consequences nor the types of receptors conveying it are precisely known. The aim of this work was to characterise the cholinergic effects on giant cells of the CN, using electrophysiology and quantitative polymerase chain reaction. Application of the cholinergic agonist carbachol increased the spontaneous activity of the giant cells; which was partly the consequence of the reduction in a K(+) conductance. This effect was mediated via M4 and M3 receptors. Cholinergic modulation also affected the synaptic transmission targeting the giant cells. Excitatory synaptic currents evoked by the stimulation of the superficial and deep regions of the CN were sensitive to cholinergic modulation: the amplitude of the first postsynaptic current was reduced, and the short-term depression was also altered. These changes were mediated via M3 receptors alone and via the combination of M4, M2 and M3 receptors, when the superficial and deep layers, respectively, were activated. Inhibitory synaptic currents evoked from the superficial layer showed short-term depression, but they were unaffected by carbachol. In contrast, inhibitory currents triggered by the activation of the deep parts exhibited no significant short-term depression, but they were highly sensitive to cholinergic activation, which was mediated via M3 receptors. Our results indicate that pre- and postsynaptic muscarinic receptors mediate cholinergic modulation on giant cells. The present findings shed light on the cellular mechanisms of a tonic cholinergic modulation in the CN, which may become particularly important in evoking contralateral excitatory responses under certain pathological conditions
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
agonist
Carbachol
CN
Cochlear Nucleus
dorsal cochlear nucleus
Electrophysiology
giant
Health
neurone
physiology
Polymerase Chain Reaction
rat
receptor
Synaptic Transmission
Megjelenés:The European Journal of Neuroscience. - 30 : 5 (2009), p. 769-782. -
További szerzők:Kőszeghy Áron (1983-) (Ph.D hallgató, élettanász) Pap Pál (1981-) (élettanász) Bakondi Gábor (1980-) (élettanász) Pocsai Krisztina (1978-) (élettanász) Szűcs Géza (1948-) (élettanász) Rusznák Zoltán (1965-) (élettanász)
Internet cím:elektronikus változat
DOI
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4.

001-es BibID:BIBFORM009113
Első szerző:Pap Pál (élettanász)
Cím:Cytoplasmic Ca2+ concentration changes evoked by cholinergic stimulation in primary astrocyte cultures prepared from the rat cochlear nucleus / Pap, P., Kőszeghy, Á., Szücs, G., Rusznák, Z.
Dátum:2009
ISSN:0378-5955
Megjegyzések:The involvement of astrocytes in the cholinergic modulation of the cochlear nucleus has been studied using primary astrocyte cultures prepared from this nucleus. The cells were loaded with the membrane permeable form of the fluorescent Ca2+ indicator Fluo-4, and carbachol-induced Ca2+ concentration increases were monitored using an imaging system. In the presence of cholinergic stimulation 36.3% of the cells produced Ca2+ transients. The time course of the transients was variable; 45.0% of the responding cells showed only a rapid Ca2+ concentration increase, while in 50.5% of the astrocytes the fast component was followed by a slow plateau phase. Using muscarine as well as general and more specific cholinergic antagonists (atropine, pirenzepine, 4-DAMP and hexamethonium), the role of the M3 and (to a smaller extent) M1 muscarinic acetylcholine receptors could be demonstrated in the genesis of the carbachol-induced Ca2+ transients. The presence of these two subtypes of muscarinic receptors has been confirmed at both mRNA (Q-PCR) and protein (immunocytochemistry) levels. Our data demonstrate the responsiveness of the cochlear astrocytes towards cholinergic stimulation, suggesting that they may have roles in mediating the effects of cholinergic modulation in the rat cochlear nucleus.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Acetylcholine
antagonist
Astrocyte
Astrocytes
Atropine
Cholinergic Antagonists
Cochlear Nucleus
Fluo-4
glia
Hexamethonium
imaging
immunochemistry
Muscarine
Muscarinic receptors
Pirenzepine
Q-PCR
rat
receptor
Time
Megjelenés:Hearing Research. - 255 : 1-2 (2009), p. 73-83. -
További szerzők:Kőszeghy Áron (1983-) (Ph.D hallgató, élettanász) Szűcs Géza (1948-) (élettanász) Rusznák Zoltán (1965-) (élettanász)
Internet cím:elektronikus változat
elektronikus változat
DOI
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5.

001-es BibID:BIBFORM001021
Első szerző:Pocsai Krisztina (élettanász)
Cím:Rhodamine backfilling and confocal microscopy as a tool for the unambiguous identification of neuronal cell types : a study of the neurones of the rat cochlear nucleus / Pocsai K., Pál B., Pap P., Bakondi G., Kosztka L., Rusznák Z., Szűcs G.
Dátum:2007
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Brain Research Bulletin. - 71 : 5 (2007), p. 529-539. -
További szerzők:Pál Balázs (1975-) (élettanász) Pap Pál (1981-) (élettanász) Bakondi Gábor (1980-) (élettanász) Kosztka Lívia (1981-) (élettanász) Rusznák Zoltán (1965-) (élettanász) Szűcs Géza (1948-) (élettanász)
Internet cím:elektronikus változat
DOI
Borító:

6.

001-es BibID:BIBFORM086030
Első szerző:Rusznák Zoltán (élettanász)
Cím:Time-dependent changes of the TASK-3 channel expression pattern of melanoma cells maintained in tissue culture : is there a connection between cell-division and TASK-3 expression? / Rusznak Z., Pocsai K., Pál Balázs Zoltán., Pap P., Csernoch László, Szűcs G.
Dátum:2005
ISSN:0022-3751 1469-7793
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
folyóiratcikk
Megjelenés:Journal of Physiology-London. - 567 : Suppl. (2005), p. 3P-4P. -
További szerzők:Pocsai Krisztina (1978-) (élettanász) Pál Balázs (1975-) (élettanász) Pap Pál (1981-) (élettanász) Csernoch László (1961-) (élettanász) Szűcs Géza (1948-) (élettanász)
Internet cím:Szerző által megadott URL
DOI
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