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001-es BibID:	BIBFORM009098
Első szerző:	Bakondi Gábor (élettanász)
Cím:	Hyperpolarization-activated, cyclic nucleotide-gated, cation non-selective channel subunit expression pattern of guinea-pig spiral ganglion cells / Bakondi, G., Por, A., Kovacs, I., Szucs, G., Rusznak, Z.
Dátum:	2009
ISSN:	0306-4522
Megjegyzések:	Although the hyperpolarization-activated non-specific cationic current (I(h)) plays important roles in determining the membrane characteristics of the spiral ganglion cells (SGCs), neither the exact types of the hyperpolarization-activated, cyclic nucleotide-gated, cation non-selective channel (HCN) subunits contributing to the molecular assembly of the relevant channels, nor their distribution pattern presented by the SGCs is known. In the present work immunolabeling and Western blot analysis were performed to describe the presence and distribution of all four known HCN subunits in the guinea-pig spiral ganglion. Besides determining the expression of the HCN1-HCN4 subunits by both type I and type II SGCs, the presence of possible apico-basal gradients in the expression patterns was also sought. The results indicate that both type I and type II SGCs express all four HCN subunits. The intensity of the immunolabeling of the cell surface membrane was generally strong, but it showed pronounced cell-to-cell variability. The Western blot experiments in combination with densitometry revealed that the amount of the HCN1 and HCN3 proteins was more significant in the apical than in the basal third of the guinea-pig cochlea. These findings not only imply potential heteromeric HCN channel formation of the spiral ganglion neurons, but they also offer a possible explanation of the previously reported heterogeneity of I(h) recorded in functional studies
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis channel Cochlea Densitometry guinea pig Health neuron Neurons physiology Proteins Spiral Ganglion
Megjelenés:	Neuroscience. - 158 : 4 (2009), p. 1469-1477. -
További szerzők:	Pór Ágnes Kovács Ilona (1965-) (patológus) Szűcs Géza (1948-) (élettanász) Rusznák Zoltán (1965-) (élettanász)
Internet cím:	elektronikus változat
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001-es BibID:	BIBFORM009112
Első szerző:	Pál Balázs (élettanász)
Cím:	Targets, receptors and effects of muscarinic neuromodulation on giant neurones of the rat dorsal cochlear nucleus / Pal, B., Koszeghy, A., Pap, P., Bakondi, G., Pocsai, K., Szucs, G., Rusznak, Z.
Dátum:	2009
ISSN:	0953-816X
Megjegyzések:	Although cholinergic modulation of the cochlear nucleus (CN) is functionally important, neither its cellular consequences nor the types of receptors conveying it are precisely known. The aim of this work was to characterise the cholinergic effects on giant cells of the CN, using electrophysiology and quantitative polymerase chain reaction. Application of the cholinergic agonist carbachol increased the spontaneous activity of the giant cells; which was partly the consequence of the reduction in a K(+) conductance. This effect was mediated via M4 and M3 receptors. Cholinergic modulation also affected the synaptic transmission targeting the giant cells. Excitatory synaptic currents evoked by the stimulation of the superficial and deep regions of the CN were sensitive to cholinergic modulation: the amplitude of the first postsynaptic current was reduced, and the short-term depression was also altered. These changes were mediated via M3 receptors alone and via the combination of M4, M2 and M3 receptors, when the superficial and deep layers, respectively, were activated. Inhibitory synaptic currents evoked from the superficial layer showed short-term depression, but they were unaffected by carbachol. In contrast, inhibitory currents triggered by the activation of the deep parts exhibited no significant short-term depression, but they were highly sensitive to cholinergic activation, which was mediated via M3 receptors. Our results indicate that pre- and postsynaptic muscarinic receptors mediate cholinergic modulation on giant cells. The present findings shed light on the cellular mechanisms of a tonic cholinergic modulation in the CN, which may become particularly important in evoking contralateral excitatory responses under certain pathological conditions
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban agonist Carbachol CN Cochlear Nucleus dorsal cochlear nucleus Electrophysiology giant Health neurone physiology Polymerase Chain Reaction rat receptor Synaptic Transmission
Megjelenés:	The European Journal of Neuroscience. - 30 : 5 (2009), p. 769-782. -
További szerzők:	Kőszeghy Áron (1983-) (Ph.D hallgató, élettanász) Pap Pál (1981-) (élettanász) Bakondi Gábor (1980-) (élettanász) Pocsai Krisztina (1978-) (élettanász) Szűcs Géza (1948-) (élettanász) Rusznák Zoltán (1965-) (élettanász)
Internet cím:	elektronikus változat DOI
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