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001-es BibID:	BIBFORM002759
Első szerző:	Lidke, Diane S.
Cím:	In vivo imaging using quantum-dot-conjugated probes / Lidke, D. S., Nagy P., Arndt-Jovin, D. J.
Dátum:	2007
Megjegyzések:	This unit describes the use of quantum dots (QDs) for live-cell imaging and the use of QDs in flow cytometry for quantitative analysis of ligand binding constants and receptor density. Conventional fluorophores and visible fluorescent protein (VFP) constructs have allowed visualization of many cellular processes. However, organic and biomolecular fluorophores have limitations in their applications, due to their small Stokes' shift and tendency to photobleach during prolonged imaging. QDs have many advantages over conventional fluorophores, including high brightness and photostability, which make them an exceptional tool for live-cell imaging. There are a large variety of commercially available QDs with different surface reactivities and characteristics. The authors have limited the laboratory protocols presented here to the use of streptavidin-coupled QDs because this gives almost universal applicability to any cell surface receptor by coupling the ligand or antibody that recognizes the receptor to biotin and visualizing the complex by use of QDs
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis Animal Animals Antibodies antibody combining site antigen antibody reaction Antigen-Antibody Reactions article Binding Sites Binding Sites,Antibody Biotinylation Cell Culture cell surface receptor Cells,Cultured Dyes dyes,reagents,indicators,markers and buffers Flow Cytometry fluorescence microscopy fluorescent dye Fluorescent Dyes Human Humans Indicators and Reagents instrumentation ligand Ligands metabolism methodology Mexico Mice Microscopy,Fluorescence microsphere Microspheres mouse quantum dot Quantum Dots Receptors,Cell Surface Streptavidin ultrastructure
Megjelenés:	Current Protocols in Cell Biology. - Supplement 36. (2007), unit 25.1.1-25.1.18. -
További szerzők:	Nagy Péter (1971-) (biofizikus) Arndt-Jovin, Donna J.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM106495
035-os BibID:	(cikkazonosító)e625 (scopus)85144584415
Első szerző:	Nagyné Szabó Ágnes Timea (vegyész)
Cím:	Principles of Resonance Energy Transfer / Szabó Ágnes, Szöllősi János, Nagy Peter
Dátum:	2022
ISSN:	2691-1299
Megjegyzések:	This unit describes the basic principles of Förster resonance energy transfer (FRET). Beginning with a brief summary of the history of FRET applications, the theory of FRET is introduced in detail using figures to explain all the important parameters of the FRET process. After listing various approaches for measuring FRET efficiency, several pieces of advice are given on choosing the appropriate instrumentation. The unit concludes with a discussion of the limitations of FRET measurements followed by a few examples of the latest FRET applications, including new developments such as spectral flow cytometric FRET, single-molecule FRET, and combinations of FRET with super-resolution or lifetime imaging microscopy and with molecular dynamics simulations.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Current Protocols. - 2 : 12 (2022), p. 1-22. -
További szerzők:	Szöllősi János (1953-) (biofizikus) Nagy Péter (1971-) (biofizikus)
Pályázati támogatás:	K138075 OTKA ANN133421 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM113275
035-os BibID:	(cikkazonosító)e835 (Scopus)85164146982
Első szerző:	Takács Roland Ádám (molekuláris biológus, biokémikus)
Cím:	Isolation and Micromass Culturing of Primary Chicken Chondroprogenitor Cells for Cartilage Regeneration / Takács Roland, Juhász Tamás, Katona Éva, Somogyi Csilla, Vágó Judit, Hajdú Tibor, Barna Krisztina Bíróné, Nagy Péter, Zákány Róza, Matta Csaba
Dátum:	2023
ISSN:	2691-1299
Megjegyzések:	Much of the skeletal system develops by endochondral ossification, a process that takes place in early fetal life. This makes the early stages of chondrogenesis, i.e., when chondroprogenitor mesenchymal cells differentiate to chondroblasts, challenging to study in vivo. In vitro methods for the study of chondrogenic differentiation have been available for some time. There is currently high interest in developing fine-tuned methodology that would allow chondrogenic cells to rebuild articular cartilage and restore joint functionality. The micromass culture system that relies on embryonic limb bud-derived chondroprogenitor cells is a popular method for the study of the signaling pathways that control the formation and maturation of cartilage. In this protocol, we describe a technique fine-tuned in our laboratory for culturing limb bud-derived mesenchymal cells from early-stage chick embryos in high density (Basic Protocol 1). We also provide a fine-tuned method for high-efficiency transient transfection of cells before plating using electroporation (Basic Protocol 2). In addition, protocols for histochemical detection of cartilage extracellular matrix using dimethyl methylene blue, Alcian blue, and safranin O are also provided (Basic Protocol 3 and Alternate Protocols 1 and 2, respectively). Finally, a step-by-step guide on a cell viability/proliferation assay using MTT reagent is also described (Basic Protocol 4). © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Micromass culture of chick embryonic limb bud-derived cells. Basic Protocol 2: Transfection of cells with siRNA constructs using electroporation prior to micromass culturing. Basic Protocol 3: Qualitative and quantitative assessment of cartilage matrix production using dimethyl methylene blue staining and image analysis. Alternate Protocol 1: Qualitative assessment of cartilage matrix production using Alcian blue staining. Alternate Protocol 2: Qualitative assessment of cartilage matrix production using safranin O staining. Basic Protocol 4: Measurement of mitochondrial activity with the MTT assay.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Current Protocols. - 3 : 7 (2023), p. 1-29. -
További szerzők:	Juhász Tamás (1976-) (biológus, orvosbiológus) Katona Éva (1986-) (molekuláris biológus) Somogyi Csilla (1983-) (biológus, angol-magyar szakfordító) Vágó Judit (1990-) (molekuláris biológus) Hajdú Tibor (1988-) (általános orvos) Barna Krisztina B. Nagy Péter (1971-) (biofizikus) Zákány Róza (1963-) (anatómus-, kötőszövetbiológus) Matta Csaba (1980-) (molekuláris biológus, genetikus, angol szakfordító)
Pályázati támogatás:	OTKA-134304 OTKA COST Egyéb
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