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001-es BibID:	BIBFORM004838
Első szerző:	Lidke, Diane S.
Cím:	Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction / Lidke, D. S., Nagy, P., Heintzmann, R., Arndt-Jovin, D., Post, J. N., Grecco, H. E., Jares-Erijman, E. A., Jovin, T. M.
Dátum:	2004
Megjegyzések:	The erbB/HER family of transmembrane receptor tyrosine kinases (RTKs) mediate cellular responses to epidermal growth factor (EGF) and related ligands. We have imaged the early stages of RTK-dependent signaling in living cells using: (i) stable expression of erbB1/2/3 fused with visible fluorescent proteins (VFPs), (ii) fluorescent quantum dots (QDs) bearing epidermal growth factor (EGF-QD) and (iii) continuous confocal laser scanning microscopy and flow cytometry. Here we demonstrate that EGF-QDs are highly specific and potent in the binding and activation of the EGF receptor (erbB1), being rapidly internalized into endosomes that exhibit active trafficking and extensive fusion. EGF-QDs bound to erbB1 expressed on filopodia revealed a previously unreported mechanism of retrograde transport to the cell body. When erbB2-monomeric yellow fluorescent protein (mYFP) or erbB3-monomeric Citrine (mCitrine) were coexpressed with erbB1, the rates and extent of endocytosis of EGF-QD and the RTK-VFP demonstrated that erbB2 but not erbB3 heterodimerizes with erbB1 after EGF stimulation, thereby modulating EGF-induced signaling. QD-ligands will find widespread use in basic research and biotechnological developments
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Animals Cell Membrane Cells chemistry Cricetinae Endocytosis Endosomes Epidermal Growth Factor Flow Cytometry Humans Ligands metabolism methods Microscopy Motion Oncogene Proteins v-erbB physiology Protein Binding Protein Interaction Mapping Protein Transport Proteins Quantum Dots Receptors, Cell Surface Research Signal Transduction Spectrometry, Fluorescence Support Tyrosine ultrastructure
Megjelenés:	Nature Biotechnology 22 : 2 (2004), p. 198-203. -
További szerzők:	Nagy Péter (1971-) (biofizikus) Heintzmann, Rainer Arndt-Jovin, Donna J. Post, Janine N. Grecco, Hernan E. Jares-Erijman, Elizabeth A. Jovin, Thomas M.
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001-es BibID:	BIBFORM004732
Első szerző:	Lidke, Diane S.
Cím:	Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET) / D. S. Lidke, P. Nagy, B. G. Barisas, R. Heintzmann, J. N. Post, K. A. Lidke, A. H. A. Clayton, D. J. Arndt-Jovin, T. M. Jovin
Dátum:	2003
ISSN:	300-5127 (Print)
Megjegyzések:	We report the implementation and exploitation of fluorescence polarization measurements, in the form of anisotropy fluorescence lifetime imaging microscopy (rFLIM) and energy migration Forster resonance energy transfer (emFRET) modalities, for wide-field, confocal laser-scanning microscopy and flow cytometry of cells. These methods permit the assessment of rotational motion, association and proximity of cellular proteins in vivo. They are particularly applicable to probes generated by fusions of visible fluorescence proteins, as exemplified by studies of the erbB receptor tyrosine kinases involved in growth-factor-mediated signal transduction.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Animals Anisotropy Cell Line Cells chemistry Cho Cells Cricetinae Dose-Response Relationship, Drug Energy Transfer Epidermal Growth Factor Flow Cytometry Fluorescence Fluorescence Polarization Green Fluorescent Proteins Humans instrumentation Luminescent Proteins metabolism methods Microscopy Microscopy,Confocal Microscopy,Fluorescence Models,Statistical Motion Mutation Proteins Receptor,Epidermal Growth Factor Research Signal Transduction Support Tyrosine
Megjelenés:	Biochemical Society Transactions 31 : Pt 5 (2003), p. 1020-1027. -
További szerzők:	Nagy Péter (1971-) (biofizikus) Barisas, B. G. Heintzmann, Rainer Post, Janine N. Lidke, K. A. Clayton, A. H. A. Arndt-Jovin, Donna J. Jovin, Thomas M.
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001-es BibID:	BIBFORM002760
Első szerző:	Lidke, Diane S.
Cím:	Biotin-ligand complexes with sreptavidin quantum dots for in vivo cell labeling of membrane receptors / Lidke, D. S., Nagy P., Jovin, T. M., Arndt-Jovin, D.
Dátum:	2007
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Quantum Dots quantum dot methods
Megjelenés:	Methods in Molecular Biology. - 374 (2007), p. 69-79. -
További szerzők:	Nagy Péter (1971-) (biofizikus) Jovin, Thomas M. Arndt-Jovin, Donna J.
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001-es BibID:	BIBFORM002759
Első szerző:	Lidke, Diane S.
Cím:	In vivo imaging using quantum-dot-conjugated probes / Lidke, D. S., Nagy P., Arndt-Jovin, D. J.
Dátum:	2007
Megjegyzések:	This unit describes the use of quantum dots (QDs) for live-cell imaging and the use of QDs in flow cytometry for quantitative analysis of ligand binding constants and receptor density. Conventional fluorophores and visible fluorescent protein (VFP) constructs have allowed visualization of many cellular processes. However, organic and biomolecular fluorophores have limitations in their applications, due to their small Stokes' shift and tendency to photobleach during prolonged imaging. QDs have many advantages over conventional fluorophores, including high brightness and photostability, which make them an exceptional tool for live-cell imaging. There are a large variety of commercially available QDs with different surface reactivities and characteristics. The authors have limited the laboratory protocols presented here to the use of streptavidin-coupled QDs because this gives almost universal applicability to any cell surface receptor by coupling the ligand or antibody that recognizes the receptor to biotin and visualizing the complex by use of QDs
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis Animal Animals Antibodies antibody combining site antigen antibody reaction Antigen-Antibody Reactions article Binding Sites Binding Sites,Antibody Biotinylation Cell Culture cell surface receptor Cells,Cultured Dyes dyes,reagents,indicators,markers and buffers Flow Cytometry fluorescence microscopy fluorescent dye Fluorescent Dyes Human Humans Indicators and Reagents instrumentation ligand Ligands metabolism methodology Mexico Mice Microscopy,Fluorescence microsphere Microspheres mouse quantum dot Quantum Dots Receptors,Cell Surface Streptavidin ultrastructure
Megjelenés:	Current Protocols in Cell Biology. - Supplement 36. (2007), unit 25.1.1-25.1.18. -
További szerzők:	Nagy Péter (1971-) (biofizikus) Arndt-Jovin, Donna J.
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