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001-es BibID:BIBFORM015624
Első szerző:Nagy Péter (biofizikus)
Cím:Distribution of resting and ligand-bound ErbB1 and ErbB2 receptor tyrosine kinases in living cells using number and brightness analysis / Nagy, P., Claus, J., Jovin, T. M., Arndt-Jovin, D. J.
Dátum:2010
Megjegyzések:Ligand-driven dimerizations of ErbB receptor subunits fulfill a fundamental role in their activation. We have used the number and brightness analysis technique to investigate the existence of preformed ligand-independent dimers and clusters and to characterize the initial steps in the activation of ErbB1 and ErbB2. In cells expressing 50,000-200,000 receptors, ErbB1 was monomeric in the absence of ligand stimulation, whereas in CHO cells with receptor levels >500,000 as much as 30% of ErbB1 was present as preformed dimers. EGF induced the formation of ErbB1 dimers as well as larger clusters (up to pentamers) that colocalized with clathrin-coated pits. The distribution of unstimulated ErbB2 in cells expressing 3.10(5)-10(6) receptors was fundamentally different, in that this receptor was present in preformed homoassociated aggregates containing 5-10 molecules. These constitutive ErbB2 homoclusters colocalized with caveolae, increased in size at subphysiological temperatures, but decreased in size upon EGF stimulation. We conclude that these ErbB2 clusters are promoted primarily by membrane-mediated interactions and are dispersed upon ligand stimulation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
A431 CELLS
ACTIVATION
analysis
article
Caveolae
Cells
Cho Cells
CORRELATION SPECTROSCOPY
CRYSTAL-STRUCTURE
Dimerization
EGFR
ENERGY-TRANSFER
Epidermal Growth Factor
EPIDERMAL-GROWTH-FACTOR
ErbB proteins
ErbB1
ErbB2
FLUORESCENCE ANISOTROPY
HIGHER-ORDER OLIGOMERS
ligand
LIPID RAFTS
LIVING CELLS
MOLECULAR-INTERACTIONS
receptor clusters
Receptor tyrosine kinase
Signal Transduction
Temperature
Tyrosine
OTKA::1
MAB::3.1
Megjelenés:Proceedings of the National Academy of Sciences of the United States of America. - 107 : 38 (2010), p. 16524-16529. -
További szerzők:Claus, J. Jovin, Thomas M. Arndt-Jovin, Donna J.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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