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001-es BibID:	BIBFORM078121
035-os BibID:	(PMID)30830246
Első szerző:	Kárai Bettina (orvos)
Cím:	A novel flow cytometric method for enhancing acute promyelocytic leukemia screening by multidimensional dot-plots / Bettina Kárai, Mira Habók, Gyula Reményi, László Rejtő, Anikó Ujfalusi, János Kappelmayer, Zsuzsanna Hevessy
Dátum:	2019
Megjegyzések:	Acute promyelocytic leukemia (APL) is generally characterized by t(15;17)(q24;q21). In some cases, the classic translocation cannot be identified by conventional methods, since the PML-RARA fusion protein results from complex, variant, or cryptic translocation. The diagnostic algorithm of APL starts with screening methods, such as flow cytometry (FC), followed by fluorescence in situ hybridization or polymerase chain reaction to confirm the diagnosis. Our aim was to develop a novel protocol for analyzing APL samples based on multidimensional dot-plots that can provide comprehensive information about several markers at the same time. The protocol included four optimized multidimensional dot-plots, which were tested by retrospective reanalysis of FC results in APL (n?=?8) and non-APL (n?=?12) acute myeloid leukemia (AML) cases. After predicting the potential position of hypergranular- and microgranular-type aberrant promyelocytes, the percentages of blast populations were examined within the gates in all AML cases. The percentage of blasts in each predefined gate was well above the cut-off value (95%) in APL cases in all tubes. In non-APL AML cases, the percentage of blasts in the same gates never reached the cut-off value in all investigated tubes, and even when it did in a single tube, the pattern was markedly different from that observed in APL cases. In conclusion, multidimensional dot-plots can be used for screening APL even in cryptic APL cases, although reproducibility across several laboratories would require standardization of antibodies and fluorochromes. This easy-to-use and quick method can support the diagnosis of APL and the prompt initiation of the appropriate treatment.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Acute promyelocytic leukemia Cryptic translocation Flow cytometry Multidimensional dot-plot
Megjelenés:	Annals of Hematology. - 98 : 6 (2019), p. 1413-1420. -
További szerzők:	Habók Mira Reményi Gyula (1969-) (belgyógyász, haematológus) Rejtő László (1963-) (belgyógyász, haematológus) Ujfalusi Anikó (1968-) (gyermekorvos, laboratóriumi szakorvos) Kappelmayer János (1960-) (laboratóriumi szakorvos) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM054366
Első szerző:	Kocsis András
Cím:	Mean corpuscular volume is not a reliable marker of red cell age in case of anisocytosis / A. Kocsis, É. Biró, É. Rácz, A. Kiss, J. Kappelmayer
Dátum:	1998
ISSN:	0939-5555
Megjegyzések:	The present study was designed to determinethe effect of anisocytosis on the association of MCVvalues with HbA1c and reticulocyte counts as markersof red cell age. Normo-, micro- and macrocytic samples,fractionated by counterflow centrifugal elutriation werestudied. The previously described correlation betweenMCV and HbA1c was only observed in normal samplesand in the middle fractions of samples with anisocytosis.At both extremes of the elutriation profile, curvesfor HbA1c content and reticulocyte count levelled out.Furthermore, in fractions containing the largest redcells of the microcytic series and the smallest red cellsof normo- and macrocytic samples, reticulocyte countdecreased while HbA1c content increased with increasingMCV. From these data it is concluded that MCV isnot an absolute determinant of red cell age in case ofanisocytosis.Key words Centrifugal elutriation 7 Meancorpuscular volume 7 Glycosylated hemoglobin 7Reticulocyte count 7 Red cell ageIntroductionTo reveal the aging process of red blood cells (RBC) amethod is required that reliably isolates them accordingto age. Previous investigations mostly used erythrocytesseparated on the basis of cell density [3]. Since the introductionof the counterflow centrifugation separationchamber, it is possible to isolate red cell fractions basedon differences in RBC volume [7, 9].The discovery that hemoglobin is non-enzymaticallyglycosylated during RBC life span has led to the conclusionthat HbA1c is a marker of red cell age [4]. Inmost studies based on counterflow centrifugal elutriationit was established that red cell age as marked byHbA1c content, decreased in parallel to an increase inMCV. Thus, it was concluded that cell age determinedRBC volume [8]. To investigate whether MCV was anabsolute determinant of red cell age, we studied a seriesof samples with anisocytosis and characterized red cellfractions of different MCV according to their HbA1cand reticulocyte content.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Annals of Hematology. - 77 : 4 (1998), p. 149-151. -
További szerzők:	Bíró Éva Rácz Éva Kiss Attila (1942-) (belgyógyász, haematológus) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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