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1.

001-es BibID:BIBFORM054689
Első szerző:Antal-Szalmás Péter (laboratóriumi szakorvos)
Cím:Measurement of soluble biomarkers by flow cytometry / Péter Antal-Szalmás, Béla Nagy Jr., Ildikó Beke Debreceni, János Kappelmayer
Dátum:2013
Megjegyzések:Microparticle based flow cytometric assays for determination of the level of soluble biomarkers are widely used in several research applications and in some diagnostic setups. The major advantages of these multiplex systems are that they can measure a large number of analytes (up to 500) at the same time reducing assay time, costs and sample volume. Most of these assays are based on antigen-antibody interactions and work as traditional immunoassays, but nucleic acid alterations - by using special hybridization probes -, enzyme- substrate or receptor-ligand interactions can be also studied with them. The applied beads are nowadays provided by the manufacturers, but cheaper biological microbeads can be prepared by any user. One part of the systems can be used on any research or clinical cytometers, but some companies provide dedicated analyzers for their multiplex bead arrays. Due to the high standardization of the bead production and the preparation of the assay components the analytical properties of these assays are quite reliable with a wide range of available applications. Cytokines, intracellular fusion protei ns, activated/phosphorylated components of different signaling pathways, transcription factors and nuclear receptors can be identified and quantitated. The assays may serve the diagnostics of autoimmune disorders, different viral and bacterial infections, as well as genetic alterations such as single nucleotide polymorphisms, small deletions/insertions or even nucleotide triplet expansions can be also identified. The most important principles, technical details and applications of these systems are discussed in this short review.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:EJIFCC [electronic resource]. - 23 : 4 (2013), p. [1-8]. -
További szerzők:Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Bekéné Debreceni Ildikó (1970-) (biológus) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM099973
Első szerző:Kappelmayer János (laboratóriumi szakorvos)
Cím:An overview on the scientometric advancement of the eJIFCC / János Kappelmayer, Harjit Pal Bhattoa, Gábor L. Kovács
Dátum:2021
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of the International Federation of Clinical Chemistry and Laboratory Medicine. - 32 : 4 (2021), p. 403-408. -
További szerzők:Bhattoa Harjit Pal (1973-) (laboratóriumi szakorvos) Kovács Gábor L. (Szeged)
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3.

001-es BibID:BIBFORM069464
Első szerző:Kappelmayer János (laboratóriumi szakorvos)
Cím:Clinical laboratories : production factories or specialized diagnostic centers / János Kappelmayer, Judit Tóth
Dátum:2016
Megjegyzések:Since a large proportion of medical decisions arebased on laboratory results, clinical laboratoriesshould meet the increasing demand of clinicians andtheir patients. Huge central laboratories may processover 10 million tests annually; they act as productionfactories, measuring emergency and routinetests with sufficient speed and accuracy. At the sametime, they also serve as specialized diagnostic centerswhere well-trained experts analyze and interpretspecial test results. It is essential to improve andconstantly monitor this complex laboratory service,by several methods. Sample transport by pneumatictube system, use of an advanced laboratory informationsystem and point-of-care testing may result indecreased total turnaround time. The optimizationof test ordering may result in a faster and more costeffectivelaboratory service. Autovalidation can savetime for laboratory specialists, when the analysis ofmore complex results requires their attention. Smallteams of experts responsible for special diagnosticwork, and their interpretative reporting according topredetermined principles, may help to minimize subjectivityof these special reports. Although laboratoryinvestigations have become so diversely developed in the past decades, it is essential that the laboratorycan provide accurate results relativelyquickly, and that laboratory specialists can supportthe diagnosis and monitoring of patientsby adequate interpretation of esoteric laboratorymethods.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
turnaround time
autovalidation
interpretative results
Megjelenés:The Electronic Journal of the International Federation of Clinical Chemistry and laboratory Medicine. - 27 : 2 (2016), p. 156-165. -
További szerzők:Tóth Judit (1978-) (laboratóriumi szakorvos)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM047530
Első szerző:Kappelmayer János (laboratóriumi szakorvos)
Cím:Prediction of therapy response and prognosis in leukemias by flow cytometric MDR assays / János Kappelmayer, Zsuzsa Hevessy, András Apjok, Katalin Tauberné Jakab
Dátum:2012
Megjegyzések:Multidrug resistance (MDR) is an unwanted phenomenon, that may cause therapy failure in several neoplasms includinghematological malignancies. The purpose of any type of laboratory MDR assay is to reliably identify such patients and to provideuseful data to clinicians with a relatively short turnaround time. MDR can be multicausal and several previous data identified agroup of transmembrane proteins - the ATP-binding casette (ABC) proteins - that may be involved in MDR in varioushematological malignancies. The prototype of these proteins is the P-glycoprotein (Pgp, MDR1, ABCB1) that is a seven-membranespanning transmembrane protein capable of extruding several cytotoxic drugs that are of key importance in the treatmentof hematological disorders. Similarly other ABC proteins ? Multidrug resistance associated protein 1 (ABCC1) and breastcancer resistance protein (ABCG2) are both capable of pumping out cytotoxic drugs. Here, we present flow cytometric methodsto identify MDR proteins by antigen and activity assays. The advantage of flow technology is the short turnaround time and itsrelative easiness compared to nucleic acid based technologies. However, for the activity assays, it should be noted, that thesefunctional tests require live cells, thus adequate results can only be provided if the specimen transport can be completed within6 hours of sample collection. Identification of MDR proteins provides prognostic information and may modulate therapy, thussignifies a clinically useful information in the evaluation of patients with leukemias.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
flow cytometry
leukemia
drug-resistance
Megjelenés:The Electronic Journal Of The International Federation Of Clinical Chemistry And Laboratory Medicine. - 23 : 4 (2012), p. 117-123. -
További szerzők:Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos) Apjok András Tauberné Jakab Katalin (Szeged)
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5.

001-es BibID:BIBFORM047531
Első szerző:Kárai Bettina (orvos)
Cím:Flow cytometry in the diagnosis of myelodysplastic syndromes / Bettina Kárai, Eszter Szánthó, János Kappelmayer, Zsuzsa Hevessy
Dátum:2012
Megjegyzések:Myelodysplastic syndromes are clonal hematopoietic stem cell disorders. Their exact etiology is unknown. Myelodysplasticsyndromes cause progressive bone marrow failure resulting in pancytopenia and refractory, transfusion-dependent anemia.One can observe typical morphological alterations in the erythroid, myeloid and/or megakaryocytic cell lineage. Blast countsmay also be increased. The pathologic cells are genetically unstable, and a myelodysplastic syndrome might transform into acutemyeloid leukemia. The overall survival of these diseases range between few months to around ten years. Correct diagnosis andaccurate prognostic classification is essential. In the past decades several scoring systems were established beginning with theFrench-American-British classification to the most recent Revised International Prognostic Scoring System. In all of theseclassifications bone marrow morphology is still the most important factor, though nowadays the genetic aberrations and flowcytometry findings are also included. The diagnosis and prognostic classification of myelodysplastic syndromes remain a greatchallenge for hematologists.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:The Electronic Journal Of The International Federation Of Clinical Chemistry And Laboratory Medicine. - 23 : 4 (2012), p. 109-116. -
További szerzők:Szánthó Eszter (laboratóriumi szakorvos jelölt) Kappelmayer János (1960-) (laboratóriumi szakorvos) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos)
Pályázati támogatás:TAMOP-4.2.2.B-11/1/KONV
TÁMOP
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6.

001-es BibID:BIBFORM069468
Első szerző:Molnár Zsuzsanna (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
Cím:Congenital hyperinsulinism caused by a de novo mutation in the ABCC8 gene : a case report / Zsuzsanna Molnár, Lídia Balogh, János Kappelmayer, László Madar, Éva Gombos, István Balogh
Dátum:2017
Megjegyzések:Congenital hyperinsulinism (CHI) is a rare genetic disordercharacterized by inappropriate insulin secretionand severe hypoglycaemia. There are two histologicalsubtypes: diffuse and focal form. Diffuse formis most common in autosomal recessive mutations inABCC8/KCNJ11 gene, while focal CHI is caused a paternallyinherited mutation and a somatic maternalallele loss.Here we report a case of a term male infant presentedwith severe hyperinsulinaemic hypoglycaemia.Gene panel testing was performed to give rapid geneticdiagnosis. We detected the c.4415-13G>A heterozygousmutation in the ABCC8 gene. Targeted genetictesting of the parents proved the de novo originof the mutation. The mutation has been previouslydescribed. The infant received octreotide treatmentand is prepared for 18-fluoro-dopa PET-CT examinationin order to localize the lesion.Rapid genetic testing might be crucial in the clinicalmanagement strategy, with decision algorithms takinginto account of the genetic status of the patient.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
ABCC8 genetic testing
hypoglycaemia
congenital hyperinsulinism
Megjelenés:The International Federation of Clinical Chemistry and Laboratory Medicine. - 8 : 28 (2017), p. 85-91. -
További szerzők:Balogh Lídia Kappelmayer János (1960-) (laboratóriumi szakorvos) Madar László (1972-) (klinikai laboratóriumi kutató) Gombos Éva (1966-) (orvosdiagnosztikai laboratóriumi analitikus) Balogh István (1972-) (molekuláris biológus, genetikus)
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7.

001-es BibID:BIBFORM054688
Első szerző:Nagy Béla Jr. (labordiagnosztikai szakorvos)
Cím:Flow cytometric investigation of classical and alternative platelet activation markers / Béla Nagy Jr., Ildikó Beke Debreceni, János Kappelmayer
Dátum:2013
Megjegyzések:Platelets show a substantial role in the maintenance of vascular integrity when these cells after a rapid activation adhere to the vessel wall lesion, aggregate with other platelets and leukocytes resulting in an arterial thrombosis. Analysis of in vivo plate let activation at an early time point is crucial in the detection of developing thrombotic events. In addition, the forecast of future complications as well as the evaluation of the efficacy of anti- platelet medication are also essential in a large group of patients. Changes in the levels of platelet receptors or alteration in other surface properties due to intra- and extracellular responses t o a stimulus can be measurable primarily by flow cytometry with specific antibodies via the assessment of classical and alternative platelet activation markers. Some of these biomarkers have been already used in routine laboratory settings in many cases, while others still stand in the phase of research applications. Deficiency in platelet receptors is also accessible with this tech nique for the diagnosis of certain bleeding disorders. We here describe the most important types of platelet activation markers, and give an overview how the levels of these markers are altered in different diseases.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:EJIFCC [electronic resource]. - 23 : 4 (2013), p. 124-134. -
További szerzők:Bekéné Debreceni Ildikó (1970-) (biológus) Kappelmayer János (1960-) (laboratóriumi szakorvos)
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8.

001-es BibID:BIBFORM083114
Első szerző:Szilágyi Bernadett
Cím:Role of sepsis modulated circulating microRNAs / Szilágyi Bernadett, Fejes Zsolt, Pócsi Marianna, Kappelmayer János, Nagy Béla Jr.
Dátum:2019
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of the International Federation of Clinical Chemistry and Laboratory Medicine. - 30 : 2 (2019), p. 128-145. -
További szerzők:Fejes Zsolt (1988-) (molekuláris biológus) Pócsi Marianna (1989-) (klinikai laboratóriumi kutató) Kappelmayer János (1960-) (laboratóriumi szakorvos) Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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9.

001-es BibID:BIBFORM091349
Első szerző:Tóth Judit (laboratóriumi szakorvos)
Cím:Detection of haemolysis, a frequent preanalytical problem in the serum of newborns and adults / Judit Tóth, Anna V. Oláh, Tamás Petercsák, Tamás Kovács, János Kappelmayer
Dátum:2020
Megjegyzések:Background: Preanalytical problems can be more frequent in case of preterm and term newborns as compared to the general patient population. Here we present the leading preanalytical errors in our laboratory, the prevalence of haemolysis and its impact on laboratory test results, and our efforts to improve the diagnostic workup of newborns' samples. Methods: Preanalytical quality indicators were analysed in all samples in 2018. The haemolysis index was measured spectrophotometrically in serum samples in the period of 2012-2018, and the ratio of haemolysed samples and the test rejection rates were analysed. The data of newborns and other patients were analysed separately. Results: During the tested year, the leading preanalytical errors were haemolysis in serum samples, inadequate sample identification and clotting of anticoagulated blood regarding all samples or newborns. In this seven-year period the ratio of haemolysed serum samples was 4.00% in all patients and 46.4% in newborns, while the test rejection rates due to haemolysis were 0.57% and 3.71%, respectively. Haemolysis indices were significantly higher in case of newborns than in patients with documented severe intravascular haemolysis which suggests that the major reason of elevated haemolysis indices in newborns was in vitro haemolysis. Accordingly, all C-reactive protein (CRP) results which were rejected by severe haemolysis became reliable after repeating blood sampling. Conclusion: Haemolysis is the leading preanalytical problem not only in newborns but also in the general patient population. Our study highlights the importance of automated assessment of serum indices and continuous monitoring of the preanalytical quality indicators and suggests the need for education and blood collection trainings.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of the International Federation of Clinical Chemistry. - 31 : 1 (2020), p. 6-14. -
További szerzők:Oláh Anna (1956-) (klinikai biokémikus, vegyész) Petercsák Tamás Kovács Tamás (1970-) (csecsemő és gyermekgyógyász, neonatológus) Kappelmayer János (1960-) (laboratóriumi szakorvos)
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