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001-es BibID:BIBFORM006863
Első szerző:Miner, Jonathan J.
Cím:Separable requirements for cytoplasmic domain of PSGL-1 in leukocyte rolling and signaling under flow / Miner, J. J., Xia, L., Yago, T., Kappelmayer, J., Liu, Z., Klopocki, A. G., Shao, B., McDaniel, J. M., Setiadi, H., Schmidtke, D. W., McEver, R. P.
Dátum:2008
Megjegyzések:In inflamed venules, leukocytes use P-selectin glycoprotein ligand-1 (PSGL-1) to roll on P-selectin and E-selectin and to activate integrin alphaLbeta2 (lymphocyte function-associated antigen-1, LFA-1) to slow rolling on intercellular adhesion molecule-1 (ICAM-1). Studies in cell lines have suggested that PSGL-1 requires its cytoplasmic domain to localize in membrane domains, to support rolling on P-selectin, and to signal through spleen tyrosine kinase (Syk). We generated "DeltaCD" mice that express PSGL-1 without the cytoplasmic domain. Unexpectedly, neutrophils from these mice localized PSGL-1 normally in microvilli, uropods, and lipid rafts. DeltaCD neutrophils expressed less PSGL-1 on their surfaces because of inefficient export from the endoplasmic reticulum. Limited digestion of wild-type neutrophils with O-sialoglycoprotein endopeptidase was used to reduce the PSGL-1 density to that on DeltaCD neutrophils. At matched PSGL-1 densities, both DeltaCD and wild-type neutrophils rolled similarly on P-selectin. However, DeltaCD neutrophils rolling on P-selectin did not trigger Syk-dependent activation of LFA-1 to slow rolling on ICAM-1. These data demonstrate that the PSGL-1 cytoplasmic domain is dispensable for leukocyte rolling on P-selectin but is essential to activate beta2 integrins to slow rolling on ICAM-1.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animals
Antigens, CD18
Hemorheology
Intercellular Adhesion Molecule-1
Intracellular Signaling Peptides and Proteins
Leukocyte Rolling
Membrane Glycoproteins
Membrane Microdomains
Mice
Mice, Knockout
Mice, Transgenic
Microvilli
Models, Biological
Models, Molecular
Molecular Sequence Data
P-Selectin
Peptide Fragments
Protein Structure, Tertiary
Protein-Tyrosine Kinases
Recombinant Proteins
Signal Transduction
Amino Acid Sequence
Megjelenés:Blood. - 112 : 5 (2008), p. 2035-2045. -
További szerzők:Xia, Lijun Yago, Tadayuki Kappelmayer János (1960-) (laboratóriumi szakorvos) Liu, Zhenghui Klopocki, Arkadiusz G. Shao, Bojing McDaniel, J. Michael Setiadi, Hendra Schmidtke, David W. McEver, Rodger P.
Internet cím:elektronikus változat
elektronikus változat
DOI
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