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001-es BibID:BIBFORM072384
Első szerző:Csongrádi Alexandra (molekuláris biológus)
Cím:Optimized angiotensin-converting enzyme activity assay for the accurate diagnosis of sarcoidosis / Alexandra Csongrádi, Attila Enyedi, István Takács, Tamás Végh , Ivetta S. Mányiné, Zsófia Pólik, István Tibor Altorjay, József Balla, György Balla, István Édes, János Kappelmayer, Attila Tóth, Zoltán Papp, Miklós Fagyas
Dátum:2018
Megjegyzések:Background:Serum angiotensin-converting enzyme (ACE) activity determination can aid the early diagnosis of sarcoidosis. We aimed to optimize a fluorescent kinetic assay for ACE activity by screening the confounding effects of endogenous ACE inhibitors and interfering factors. Genotype-dependent and genotype-independent reference values of ACE activity were established, and their diagnostic accuracies were validated in a clinical study.Methods:Internally quenched fluorescent substrate, Abz-FRK(Dnp)P-OH was used for ACE-activity measurements. A total of 201 healthy individuals and 59 presumably sarcoidotic patients were enrolled into this study. ACE activity and insertion/deletion (I/D) genotype of the ACE gene were determined.Results:Here we report that serum samples should be diluted at least 35-fold to eliminate the endogenous inhibitor effect of albumin. No significant interferences were detected: up to a triglyceride concentration of 16 mM, a hemoglobin concentration of 0.71 g/L and a bilirubin concentration of 150 ?M. Genotype-dependent reference intervals were considered as 3.76?11.25 U/L, 5.22?11.59 U/L, 7.19?14.84 U/L for II, ID and DD genotypes, respectively. I/D genotype-independent reference interval was established as 4.85?13.79 U/L. An ACE activity value was considered positive for sarcoidosis when it exceeded the upper limit of the reference interval. The optimized assay with genotype-dependent reference ranges resulted in 42.5% sensitivity, 100% specificity, 100% positive predictive value and 32.4% negative predictive value in the clinical study, whereas the genotype-independent reference range proved to have inferior diagnostic efficiency.Conclusions:An optimized fluorescent kinetic assay of serum ACE activity combined with ACE I/D genotype determination is an alternative to invasive biopsy for confirming the diagnosis of sarcoidosis in a significant percentage of patients.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
ACE
angiotensin-converting enzyme
genotype
reference interval
sarcoidosis
Megjelenés:Clinical chemistry and laboratory medicine. - 56 : 7 (2018), p. 1117-1125. -
További szerzők:Enyedi Attila (1975-) (sebész) Takács István (1963-) (sebész) Végh Tamás (1975-) (aneszteziológus, intenzív terápiás szakorvos) Mányiné Siket Ivetta (1962-) (laborasszisztens) Pólik Zsófia Altorjay István (1991-) (orvos, kardiológus) Balla József (1959-) (belgyógyász, nephrológus) Balla György (1953-) (csecsemő és gyermekgyógyász, neonatológus) Édes István (1952-) (kardiológus) Kappelmayer János (1960-) (laboratóriumi szakorvos) Tóth Attila (1971-) (biológus) Papp Zoltán (1965-) (kardiológus, élettanász) Fagyas Miklós (1984-) (orvos)
Pályázati támogatás:PD 116212
NKFIH
K 116940
OTKA
ÚNKP-17-4-I-DE-40
ÚNKP
GINOP-2.3.2-15-2016-00043
GINOP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM074180
035-os BibID:(cikkazonosító)e0197890 (WOS)000434786600008 (Scopus)85048322440
Első szerző:Fejes Zsolt (molekuláris biológus)
Cím:Endothelial cell activation is attenuated by everolimus via transcriptional and post-transcriptional regulatory mechanisms after drug-eluting coronary stenting / Fejes Z., Czimmerer Z., Szük T., Póliska S., Horváth A., Balogh E., Jeney V., Váradi J., Fenyvesi F., Balla G., Édes I., Balla J., Kappelmayer J., Nagy B. Jr.
Dátum:2018
ISSN:1932-6203
Megjegyzések:We previously found higher level of endothelial cell (EC) activation in patients who suffered from in-stent restenosis after bare-metal stenting compared to subjects who underwent drug-eluting stenting (DES) showing no complications. Here we investigated the potential transcriptional and post-transcriptional regulatory mechanisms by which everolimus attenuated EC activation after DES. We studied the effect of everolimus on E-selectin (SELE) and VCAM1 mRNA levels when human coronary artery (HCAECs) and human umbilical vein ECs were challenged with recombinant TNF-? (100 ng/mL) for 1-24 hours in the presence or absence of everolimus using 0.5 ?M concentration locally maintained by DES. EC activation was evaluated via the levels of IL-1? and IL-6 mRNAs with miR-155 expression by RT-qPCR as well as the nuclear translocation of nuclear factor kappa beta (NF-?B) detected by fluorescence microscopy. To investigate the transcriptional regulation of E-selectin and VCAM-1, TNF-?-induced enhancer RNA (eRNA) expression at p65-bound enhancers in the neighboring genomic regions of SELE and VCAM1 genes, including SELE_-11Kb and VCAM1_-10Kb, were measured in HCAECs. Mature and precursor levels of E-selectin and VCAM-1 repressor miR-181b were quantified to analyze the post-transcriptional regulation of these genes in HCAECs. Circulating miR-181b was analyzed in plasma samples of stented subjects by stem-loop RT-qPCR. TNF-? highly elevated E-selectin and VCAM-1 expression at transcriptional level in ECs. Levels of mature, pre- and pri-miR-181b were repressed in ECs by TNF-?, while everolimus acted as a negative regulator of EC activation via inhibited translocation of NF-?B p65 subunit into cell nuclei, lowered eRNA expression at SELE and VCAM1 genes-associated enhancers and modulated expression of their post-transcriptional repressor miR-181b. Significant negative correlation was observed between plasma miR-181b and soluble E-selectin and VCAM-1 in patients. In conclusion, everolimus attenuates EC activation via reduced NF-?B p65 translocation causing decreased E-selectin and VCAM-1 expression at transcriptional and post-transcriptional level after DES.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
coronary stenting
endothelial cell
enhancer RNA
everolimus
inflammation
microRNA
Megjelenés:Plos One. - 13 : 6 (2018), p. 1-20. -
További szerzők:Czimmerer Zsolt (1981-) (molekuláris biológus) Szűk Tibor (1967-) (kardiológus) Póliska Szilárd (1978-) (biológus) Horváth Attila (1988-) (programtervező informatikus) Balogh Enikő (1987-) (molekuláris biológus) Jeney Viktória (1971-) (vegyész, kémia tanár) Váradi Judit (1973-) (gyógyszerész, gyógyszertechnológus) Fenyvesi Ferenc (1977-) (gyógyszerész, gyógyszertechnológus) Balla György (1953-) (csecsemő és gyermekgyógyász, neonatológus) Édes István (1952-) (kardiológus) Balla József (1959-) (belgyógyász, nephrológus) Kappelmayer János (1960-) (laboratóriumi szakorvos) Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos)
Pályázati támogatás:GINOP-2.3.2-15-2016-00043
GINOP
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM054156
Első szerző:Szűk Tibor (kardiológus)
Cím:Effects of ad hoc clopidogrel loading versus pre-treatment on P-selectin expression after coronary stent implantation / Tibor Szuk, Béla Nagy, Zsuzsanna Bereczky, Zsolt Koszegi, István Edes, János Kappelmayer
Dátum:2006
ISSN:0953-7104
Megjegyzések:Stents represent a thrombogenic surface where one of the earliest events is the deposition of platelets. Activated platelets express neoepitopes that enable them to form homo- and heterotypic aggregates and activate the coagulation system. In this study, we investigated the effects of ad hoc clopidogrel treatment compared to a clopidogrel pretreated group on platelet P-selectin expression. We found that after stent implantation in the ad hoc treated group, P-selectin expression decreased after 1 h, while the initial decrease in platelet count normalized after 4 h. Aspirin non-responsiveness did not influence the results. These data point to the beneficial effect of clopidogrel treatment in reducing platelet reactivity during stenting.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Drug Delivery Systems
Flow Cytometry
Humans
P-Selectin
Platelet Activation
Platelet Aggregation Inhibitors
Stents
Ticlopidine
Megjelenés:Platelets. - 17 : 5 (2006), p. 344-346. -
További szerzők:Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Bereczky Zsuzsanna (1974-) (orvosi laboratóriumi diagnosztika szakorvos) Kőszegi Zsolt (1962-) (kardiológus, belgyógyász) Édes István (1952-) (kardiológus) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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