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1.

001-es BibID:BIBFORM117407
035-os BibID:(WoS)001143857500001 (Scopus)85180605811
Első szerző:Ghansah, Harriet (PhD)
Cím:Low factor XIII levels and altered fibrinolysis in patients with multiple myeloma / Harriet Ghansah, Rita Orbán-Kálmándi, Ildikó Beke Debreceni, Éva Katona, László Rejtő, László Váróczy, Linda Lóczi, Bas de Laat, Dana Huskens, János Kappelmayer, Zsuzsa Bagoly
Dátum:2024
ISSN:0049-3848
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Thrombosis Research. - 234 (2024), p. 12-20. -
További szerzők:Orbán-Kálmándi Rita Angéla (1993-) (klinikai laboratóriumi kutató) Bekéné Debreceni Ildikó (1970-) (biológus) Katona Éva (1961-) (klinikai biokémikus) Rejtő László (1963-) (belgyógyász, haematológus) Váróczy László (1974-) (belgyógyász, haematológus) Lóczi Linda Laat, Bas de Huskens, Dana Kappelmayer János (1960-) (laboratóriumi szakorvos) Bagoly Zsuzsa (1978-) (orvos)
Internet cím:Szerző által megadott URL
DOI
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2.

001-es BibID:BIBFORM034578
Első szerző:Kappelmayer János (laboratóriumi szakorvos)
Cím:Coagulation factor XIII-A. A flow cytometric intracellular marker in the classification of acute myeloid leukemias / Kappelmayer, J., Simon, A., Katona, E., Szanto, A., Nagy, L., Kiss, A., Kiss, C., Muszbek, L.
Dátum:2005
ISSN:0340-6245
Megjegyzések:The association of coagulation factors with leukocytes have been demonstrated in several previous studies. This study was designed to study the sensitivity and specificity of factor XIII subunit A (FXIII-A) labelling in cultured myeloblastic and monoblastic cell lines and to investigate the intracytoplasmic expression of FXIII-A in de novo acute myeloid leukemia (AML) samples. Myeloblastic and a monoblastic cell lines were cultured and investigated for lineage specific maturation markers and FXIII-A expression. Furthermore, FXIII-A expression was investigated in 12 normal samples (7 bone marrow and 5 peripheral blood), 86 de novo AML samples and 6 chronic myelomonocytic leukemia (CMML) samples. In the monoblastic MonoMac6 cell line the appearance of FXIII-A preceded that of CD14 while it remained negative in the myeloblastic PLB-985 cell line throughout its maturation period. Among the AML samples the average frequency of FXIII-A positive cells in myeloblastic leukemia samples was below 10%, while in M4 and M5AML samples it was above 50% and was significantly higher than the generally used CD14 marker (p < 0.0001). In the AML M4 and M5 cases, FXIII-A proved sensitive for the identification of monoblasts. FXIII-A can be considered as a reliable intracytoplasmic marker for the monocytic and megakaryocytic series and its presence is highly predictive for mono- and megakaryocytic AML and for CMML.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Thrombosis And Haemostasis. - 94 : 2 (2005), p. 454-459. -
További szerzők:Simon Ágnes (1969-) (laboratóriumi szakorvos) Katona Éva (1961-) (klinikai biokémikus) Szántó Attila (1976-) (orvos, biokémikus) Nagy László (1966-) (molekuláris sejtbiológus, biokémikus) Kiss Attila (1942-) (belgyógyász, haematológus) Kiss Csongor (1956-) (hematológus, onkológus) Muszbek László (1942-) (haematológus, kutató orvos)
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM006765
Első szerző:Katona Éva (klinikai biokémikus)
Cím:Factor XIII in bronchoalveolar lavage fluid from children with chronic bronchoalveolar inflammation / Katona, E., Nagy, B., Kappelmayer, J., Baktai, G., Kovacs, L., Marialigeti, T., Dezso, B., Muszbek, L.
Dátum:2005
ISSN:1538-7933
Megjegyzések:Extravascular activation of the coagulation system and consequent fibrin deposition is involved in the pathomechanism of chronic bronchoalveolar inflammatory diseases. The turnover of extravascular fibrin is attenuated by its cross-linking with activated factor XIII (FXIII). OBJECTIVES: Determination of cellular and plasmatic forms of FXIII and their correlation with D-dimer level in the bronchoalveolar lavage fluid (BALF) from healthy children and from children with bronchoalveolar inflammation. PATIENTS AND METHODS: Highly sensitive immunoassays were used for the quantitation of cellular and plasma FXIII and D-dimer in the BALF of children with recurrent wheezy bronchitis and fibrosing alveolitis. BALF was investigated for FXIII-containing cells by flow cytometry. RESULTS AND CONCLUSIONS: In the BALF of controls a low amount of the cellular form of FXIII (FXIII A2) and D-dimer were measured, while plasma FXIII (FXIII A2B2) was absent. Alveolar macrophages represented the single cell population in BALF that contained FXIII. In the BALF of both patients' groups the concentration and the total amount of FXIII A2 was significantly elevated, and plasma FXIII also appeared in the BALF of most patients. The D-dimer concentration was also elevated in the patients' groups and it correlated both with plasma FXIII and neutrophil count. These findings suggest that FXIII A2 is released from activated or injured alveolar macrophages into the bronchoalveolar lining fluid and in bronchoalveolar inflammatory diseases, FXIII A2B2 also leaks out from the capillaries. By cross-linking fibrin and inhibitors of fibrinolysis to fibrin, FXIII might be a key regulator of fibrin turnover in the extravascular compartment.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Adolescent
Bronchi
Bronchitis
Bronchoalveolar Lavage Fluid
Capillaries
Child
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Female
Fibrinolysis
Flow Cytometry
Humans
Infant
Inflammation
Macrophages
Male
Neutrophils
Pulmonary Alveoli
Time Factors
Megjelenés:Journal of Thrombosis and Haemostasis. - 3 : 7 (2005), p. 1407-1413. -
További szerzők:Nagy Béla (1949-) (csecsemő- és gyermekgyógyász, gyermek-tüdőgyógyász) Kappelmayer János (1960-) (laboratóriumi szakorvos) Baktai György Kovács L. (Budapest orvos) Marialigeti Tivadar Dezső Balázs (1951-) (pathológus) Muszbek László (1942-) (haematológus, kutató orvos)
Internet cím:elektronikus változat
DOI
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4.

001-es BibID:BIBFORM053643
Első szerző:Kiss Flóra (bőrgyógyász)
Cím:Leukemic lymphoblasts, a novel expression site of coagulation factor XIII subunit A / Flóra Kiss, Zsuzsanna Hevessy, Anikó Veszprémi, Éva Katona, Csongor Kiss, György Vereb, László Muszbek, János Kappelmayer
Dátum:2006
Megjegyzések:Blood coagulation factor XIII (FXIII) is a protransglutaminasecirculating as a tetramer formed by two types of subunits (A2B2).The intracellular dimeric form of FXIII (A2) is present in platelets,megakaryocytes, monocytes and macrophages and hasbeen detected in mono- and megakaryocytic leukemias.The aimof our study was to investigate FXIII-A expression in newly diagnosedB cell acute lymphoblastic leukemia (ALL) samples. Weexamined 47 de novo ALL cases of B cell origin by triple colorlabeling with flow cytometry. FXIII-A was detected by a FITCconjugated monoclonal antibody combined with CD34 andCD45 staining. In selected cases FXIII-A was investigated onslides prepared from blasts and visualized with a fluorescentmicroscope. In addition, blasts were studied by Western blotanalysis and FXIII-A was measured by a highly sensitive ELISAmethod. By flow cytometry 19 samples of the 47 cases werefound to be FXIII-A positive. Antigen concentration was 3.11 ?1.19 fg/blast, while normal lymphoid precursors and maturelymphocytes from B-CLL did not contain FXIII-A.In the lysate oflymphoblasts that were positive by flow cytometry, a single band(82 kDa) corresponding to FXIII-A was detected on Westernblots. Confocal laser scanning microscopic examination revealedthe presence of FXIII-A in the cytoplasm of these lymphoblasts.This novel expression site of FXIII-A in leukemic lymphoblastscan be utilized as a diagnostic tool and may also gain functionalsignificance in B-lineage ALL.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Coagulation factor XIII
acute lymphoblastic leukemia
flow cytometry
Megjelenés:Thrombosis Haemostasis. - 96 : 2 (2006), p. 176-182. -
További szerzők:Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos) Veszprémi Anikó Katona Éva (1961-) (klinikai biokémikus) Kiss Csongor (1956-) (hematológus, onkológus) Vereb György (1965-) (biofizikus, orvos) Muszbek László (1942-) (haematológus, kutató orvos) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM005605
Első szerző:Kiss Flóra (bőrgyógyász)
Cím:A coagulation factor becomes useful in the study of acute leukemias : studies with blood coagulation factor XIII / Kiss F., Simon Á., Csáthy L., Hevessy Z., Katona É., Kiss C., Kappelmayer J.
Dátum:2008
Megjegyzések:The intracellular form of the coagulation factor XIII has previously been identified by immunomorphological techniques using polyclonal antibodies. In these studies, only the A subunit (FXIII-A) was detectable in megakaryocytes/platelets and in monocytes/ macrophages. We developed several novel monoclonal antibody clones directed to both subunits (FXIII-A and FXIII-B) and investigated their appearance in normal and leukemic cells. By using 3- and 4-color flow cytometry FXIII expression was investigated in normal peripheral blood and bone marrow samples and in acute myeloblastic (AML) and lymphoblastic (ALL) leukemia cases. Samples were studied by Western blotting and confocal laser scanning microscopy. With a previously published ELISA assay applying two monoclonal antibodies directed to different epitopes in FXIII-A, we were able to measure the intracytoplasmic content of FXIII-A in normal cells and leukemic blasts. FXIII-A was detectable in normal peripheral blood monocytes and in large quantities in platelets, but both cell types were negative for FXIII-B. There was no surface staining for FXIII-A, it only appeared intracellularly. In samples derived from patients with AML M4 and M5, FXIII-A sensitively identified blast cells. Although normal lymphocytes do not express FXIII-A, 40% of ALL cases showed significant FXIII-A expression as determined by flow cytometry. FXIII-A positivity of lymphoblasts was verified by Western blotting, ELISA, and confocal laser scanning microscopy cytometry. These data provide evidence that FXIII-A is a sufficiently sensitive marker in differentiating myeloblasts and monoblasts and is suitable for identifying leukemia-associated phenotypes in ALL.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
factor XIII
flow cytometry
acute leukemia phenotype
Megjelenés:Cytometry. Part A. - 73A : 3 (2008), p. 194-201. -
További szerzők:Simon Ágnes (1969-) (laboratóriumi szakorvos) Csáthy László (1979-) (laboratóriumi szakorvos) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos) Katona Éva (1961-) (klinikai biokémikus) Kiss Csongor (1956-) (hematológus, onkológus) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Internet cím:DOI
elektronikus változat
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6.

001-es BibID:BIBFORM034576
Első szerző:Simon Ágnes (laboratóriumi szakorvos)
Cím:Expression of coagulation factor XIII subunit A in acute promyelocytic leukemia / Ágnes Simon, Zsuzsa Bagoly, Zsuzsanna Hevessy, László Csáthy, Éva Katona, György Vereb, Anikó Ujfalusi, László Szerafin, László Muszbek, János Kappelmayer
Dátum:2012
ISSN:1552-4949
Megjegyzések:Leukemic cells often express markers which are not characteristic of their particular cell lineage. In this study we identified the "A" subunit of coagulation factor XIII (FXIII-A) in leukemic promyelocytes in de novo AML M3 cases. The cytoplasmic presence of factor XIII-A has previously been shown only in platelets/megakaryocytes and monocytes/macrophages. Furthermore, more recently we described the presence of FXIII-A in leukemic lymphoblasts. We studied 14 patients with this rare type of acute leukemia in a period of 4 years and investigated their bone marrow samples by 3-color flow cytometry upon diagnosis, mainly focusing on FXIII-A expression of leukemic cells. We detected FXIII-A also by ELISA, Western-blot and confocal laser scanning microscopy. This was a homogenous group of AML M3 patients with translocation t(15;17)(q22;q21) detected by fluorescence in situ hybridization (FISH). In 10 out of 14 samples, FXIII-A was detectable by flow cytometry and was coexpressed with markers characteristic for leukemic promyleocytes (CD45dim/CD13+/CD33+/CD117+/cyMPO+ and HLA-DR-/CD34-/CD14-/CD15-). Staining for the markers GPIIb and GPIX were negative, and FXIII-A was identified in the cytoplasm of the cells by confocal microscopy in a relatively high quantity, as measured by ELISA. By Western blot analysis we could identify FXIII-A in the native 82 kD form and in cleaved forms corresponding to cleavage products observed when purified FXIII-A was treated by human neutrophil elastase. Since normal promyelocytes were FXIII-A negative, this novel expression site of FXIII-A in AML M3 can be considered as a leukemia associated immunophenotype and may have pathophysiological significance.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Molekuláris Medicina
Megjelenés:Cytometry. Part B. Clinical Cytometry. - 82B : 4 (2012), p. 209-216. -
További szerzők:Bagoly Zsuzsa (1978-) (orvos) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos) Csáthy László (1979-) (laboratóriumi szakorvos) Katona Éva (1961-) (klinikai biokémikus) Vereb György (1965-) (biofizikus, orvos) Ujfalusi Anikó (1968-) (gyermekorvos, laboratóriumi szakorvos) Szerafin László (1958-) (belgyógyászat, haematológia, klinikai onkológia szakorvos) Muszbek László (1942-) (haematológus, kutató orvos) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Pályázati támogatás:TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
Celluláris hematológia - immunológia
TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
A véralvadás XIII-as faktorának (FXIII) struktúrája, funkciója, előfordulása egyéb testnedvekben és kapcsolata trombotikus megbetegedésekkel
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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