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001-es BibID:BIBFORM103023
035-os BibID:(cikkazonosító)935902 (WoS)000843102800001 (Scopus)85136210735
Első szerző:Fekete Erzsébet (biotechnológus, egyetemi tanár)
Cím:Bioreactor as the root cause of the "manganese effect" during Aspergillus niger citric acid fermentations / Fekete Erzsébet, Bíró Vivien, Márton Alexandra, Bakondi-Kovács István, Németh Zoltán Sándor Erzsébet, Kovács Béla, Fábián István, Kubicek Christian P., Tsang Adrian, Karaffa Levente
Dátum:2022
ISSN:2296-4185
Megjegyzések:High-yield citric acid production by the filamentous Ascomycete fungus Aspergillus niger requires a combination of extreme nutritional conditions, of which maintaining a low manganese (II) ion concentration (<5 ?g L?1 ) is a key feature. Technical-scale production of citric acid predominantly uses stainless- steel tank fermenters, but glass bioreactors used for strain improvement and manufacturing process development also contain stainless steel components, in which manganese is an essential alloying element. We show here that during citric acid fermentations manganese (II) ions were leaching from the bioreactor into the growth media, resulting in altered fungal physiology and morphology, and significant reduction of citric acid yields. The leaching of manganese (II) ions was dependent on the fermentation time, the acidity of the culture broth and the sterilization protocol applied. Manganese (II) ion leaching was partially mitigated by electrochemical polishing of stainless steel components of the bioreactor. High concentrations of manganese (II) ions during early cultivation led to a reduction in citric acid yield. However, the effect of manganese (II) ions on the reduction of citric acid yield diminished towards the second half of the fermentation. Since maintaining low concentrations of manganese (II) ions is costly, the results of this study can potentially be used to modify protocols to reduce the cost of citric acid production.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Aspergillus niger
manganese ions
citric acid
stainless steel
metal ions leaching
fungal morphology
Megjelenés:Frontiers in Bioengineering and Biotechnology. - 10 (2022), p. 1-12. -
További szerzők:Bíró Vivien (1994-)c(mikrobiológus) Márton Alexandra (1994-) (mikrobiológus) Bakondi-Kovács István (1963-) (mikrobiológus) Németh Zoltán (1987-) (biomérnök) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Kovács Béla (1963-) (okleveles vegyész, angol szakfordító) Fábián István (1956-) (vegyész) Kubicek, Christian P. Tsang, Adrian Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
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001-es BibID:BIBFORM093531
035-os BibID:(cikkazonosító)680420 (WOS)000657282100001 (Scopus)85107225932
Első szerző:Karaffa Erzsébet Mónika (mikrobiológus, egyetemi tanár)
Cím:Carbon-source dependent interplay of copper and manganese ions modulates the morphology and itaconic acid production in Aspergillus terreus / Erzsébet Sándor, István S. Kolláth, Erzsébet Fekete, Vivien Bíró, Michel Flipphi, Béla Kovács, Christian P. Kubicek, Levente Karaffa
Dátum:2021
ISSN:1664-302X
Megjegyzések:The effects of the interplay of copper (II) and manganese (II) ions on growth, morphology and itaconic acid formation was investigated in a high-producing strain of Aspergillus terreus (NRRL1960), using carbon sources metabolized either mainly via glycolysis (D-glucose, D-fructose) or primarily via the pentose phosphate shunt (D-xylose, L-arabinose). Limiting Mn2+ concentration in the culture broth is indispensable to obtain high itaconic acid yields, while in the presence of higher Mn2+ concentrations yield decreases and biomass formation is favored. However, this low yield in the presence of high Mn2+ ion concentrations can be mitigated by increasing the Cu2+ concentration in the medium when D-glucose or D-fructose is the growth substrate, whereas this effect was at best modest during growth on D-xylose or -arabinose. A. terreus displays a high tolerance to Cu2+ which decreased when Mn2+ availability became increasingly limiting. Under such conditions biomass formation on D-glucose or D-fructose could be sustained at concentrations up to 250 mg L-1 Cu2+, while D-xylose- or L-arabinose supported growth was fully inhibited already at 100 mg L-1, while on D-xylose- or L-arabinose biomass formation was completely inhibited at 100 mg L-1. High (>75%) specific molar itaconic acid yields always coincided with an "overflow-associated" morphology, characterized by small compact pellets (<250 mm diameter) and short chains of "yeast-like" cells that exhibit increased diameters relative to the elongated cells in growing filamentous hyphae. At low concentrations (?1 mg L-1) of Cu2+ ions, manganese deficiency did not prevent filamentous growth. Mycelial- and cellular morphology progressively transformed into the typical overflow-associated one when external Cu2+ concentrations increased, irrespective of the available Mn2+. Our results indicate that copper ions are relevant for overflow metabolism and should be considered when optimizing itaconic acid fermentation in A. terreus.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Aspergillus terreus
Manganese(II) ions
Copper(II) ions
itaconic acid overflow
Pentose, hexose
Mycelial pellets
divalent cation antagonism
Megjelenés:Frontiers in Microbiology. - 12 (2021), p. 1-14. -
További szerzők:Kolláth István Sándor (biomérnök) Fekete Erzsébet (1975-) (biotechnológus, egyetemi tanár) Bíró Vivien (1994-)c(mikrobiológus) Flipphi, Michel (1964-) Kovács Béla (1963-) (okleveles vegyész, angol szakfordító) Kubicek, Christian P. Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
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