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1.

001-es BibID:BIBFORM001280
Első szerző:Bánfalvi Gáspár (sejtbiológus, gyógyszerész)
Cím:Cell culture density dependent toxicity and chromatin changes upon cadmium treatment in murine pre-B-cells / Gaspar Banfalvi, Kinga Ujvarosi, Gyorgy Trencsenyi, Csilla Somogyi, Gabor Nagy, Alexei Basnakian
Dátum:2007
Megjegyzések:Murine pre-B-cells grown in the presence of lower (1 microM) or higher (5 microM) concentration of cadmium chloride were separated into 13 fractions by centrifugal elutriation. The rate of DNA synthesis after cadmium treatment determined in permeable cells was dependent on cell culture density during cadmium treatment. Cell cycle analysis revealed a shift in the profile of DNA synthesis from replicative to repair DNA synthesis upon cadmium treatment. The study of the relationship between cell culture density and cell diameter at lower and higher cell densities in the presence of 1 microM cadmium chloride concentration showed that a. at 5 x 10(5) cell/ml or lower densities cells were shrinking indicating apoptotic changes, b. at higher cell culture densities the average cell size increased, c. the treatment of cells with low CdCl(2) concentration (1 microM) at higher cell culture density (>5 x 10(5) cell/ml) did not change significantly the average cell diameter. At 5 microM cadmium concentration and higher cell culture densities (>5 x 10(5) cell/ml) the average cell size decreased in each elutriated fraction. Most significant inhibition of cell growth took place in early S phase (2.0-2.5 C value). Apoptotic chromatin changes in chromatin structure after cadmium treatment were seen as large extensive disruptions, holes in the nuclear membrane and stickiness of incompletely folded chromosomes.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
kadmium toxicitás
kromatin szerkezet változás
reverzibilis premeabilizálás
sejt szinkronizálás
Megjelenés:Apoptosis. - 12 : 7 (2007), p. 1219-1228. -
További szerzők:Ujvárosi Kinga Trencsényi György (1978-) (biológus, biokémikus, molekuláris biológus) Somogyi Csilla (1983-) (biológus, angol-magyar szakfordító) Szemán-Nagy Gábor (1975-) (biológia tanár-molekuláris biológus) Basnakian, Alexei G.
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2.

001-es BibID:BIBFORM001285
Első szerző:Bánfalvi Gáspár (sejtbiológus, gyógyszerész)
Cím:Supranucleosomal organization of chromatin fibers in nuclei of Drosophila S2 cells / Gaspar Banfalvi, Gyorgy Trencsenyi, Kinga Ujvarosi, Gabor Nagy, Timea Ombodi, Monika Bedei, Csilla Somogyi, Alexei G. Basnakian
Dátum:2007
Megjegyzések:Earlier, the interphase chromatin structures could not be visualized due to the stickiness of the nuclear material. We have reduced stickiness by the reversal of permeabilization allowing the isolation and microscopic imaging of interphase chromatin structures. By using a high resolution of synchronization, collecting 36 elutriation fractions, we show that major intermediates of chromatin condensation include: (a) decondensed veillike chromatin at the unset of the S phase (2.0-2.2 C-value), (b) polarization of veiled chromatin (2.2-2.6 C), (c) fibrous chromatin (2.6-3.0 C), chromatin bodies (3.0-3.3 C), early precondensed chromosomes (3.3-3.6). The compaction of Drosophila chromosomes did not reach that of the mammalian cells in the final stage of condensation (3.6-4.0 C). Drosophila chromosomes consist of smaller units called rodlets. Results demonstrate that nucleosomal chromatin ("beads on string") does not form a solenoid structure; rather, the topological arrangement consists of meandering and plectonemic loops.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
kromatin szál
magasabb rendű szerkezet
kromatin rudacskák
Megjelenés:DNA and Cell Biology. - 26 : 1 (2007), p. 55-62. -
További szerzők:Trencsényi György (1978-) (biológus, biokémikus, molekuláris biológus) Ujvárosi Kinga Szemán-Nagy Gábor (1975-) (biológia tanár-molekuláris biológus) Ombodi Timea Bedei Mónika Somogyi Csilla (1983-) (biológus, angol-magyar szakfordító) Basnakian, Alexei G.
Internet cím:elektronikus változat
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3.

001-es BibID:BIBFORM001289
Első szerző:Bánfalvi Gáspár (sejtbiológus, gyógyszerész)
Cím:Cadmium induced apoptotic changes in chromatin structure and subphases of nuclear growth during the cell cycle in CHO cells / G. Banfalvi, M. Gacsi, G. Nagy, Z. B. Kiss, A. G. Basnakian
Dátum:2005
ISSN:1360-8185 1573-675X
Megjegyzések:CHO cells were grown in the presence of 1 mu M CdCl(2) and subjected to ATP-dependent replicative DNA synthesis after permeabilization. By decreasing the density of the cell culture replicative DNA synthesis was diminishing. At higher than 2 x 10(6) cell/ml concentration Cd had virtually no effect on the rate of DNA replication. Growth at higher cell concentrations could be suppressed by increasing Cd concentration. After Cd treatment cells were synchronized by counterflow centrifugal elutriation. Cadmium toxicity on cell growth in early and mid S phase led to the accumulation of enlarged cells in late S phase. Flow cytometry showed increased cellular and nuclear sizes after Cd treatment. As the cells progressed through the S phase, 11 subpopulations of nuclear sizes were distinguished. Apoptotic chromatin changes were visualized by fluorescent microscopy in a cell cycle dependent manner. In the control untreated cells the main transitory forms of chromatin corresponded to those we have published earlier (veil-like, supercoiled chromatin, fibrous, ribboned structures, chromatin strings, elongated prechromosomes, precondensed chromosomes). Cadmium treatment caused: (a) the absence of decondensed veil-like structures and premature chromatin condensation in the form of apoptotic bodies in early S phase (2.2-2.4 average C-value), (b) the absence of fibrous structures, the lack of supercoiled chromatin, the appearance of uncoiled ribboned chromatin and perichromatin semicircles, in early mid S phase (2.5-2.9 C), (c) the presence of perichromatin fibrils and chromatin bodies in mid S phase (2.9-3.2 C), (d) early intra-nuclear inclusions, elongated forms of premature chromosomes, the extrusion and rupture of nuclear membrane later in mid S phase (3.3-3.4 C), (e) the exclusion of chromatin bodies and the formation of clusters of large-sized perichromatin granules in late S phase (3.5-3.8 C) and (f) large extensive disruptions and holes in the nuclear membrane and the clumping of incompletely folded chromosomes (3.8-4. C).
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
kadmium
apoptózis
kromatin változás
sejtmag növekedés fázisai
Megjelenés:Apoptosis. - 10 : 3 (2005), p. 631-642. -
További szerzők:Gácsi Mariann Szemán-Nagy Gábor (1975-) (biológia tanár-molekuláris biológus) Kiss Zsigmond, B. Basnakian, Alexei G.
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4.

001-es BibID:BIBFORM001219
035-os BibID:(Wos)000237853100005 (Scopus)33744794924
Első szerző:Bánfalvi Gáspár (sejtbiológus, gyógyszerész)
Cím:Common pathway of chromosome condensation in mammalian cells / Gaspar Banfalvi, Gabor Nagy, Mariann Gacsi, Tamas Roszer, Alexei G. Basnakian
Dátum:2006
Megjegyzések:During evolution ribose was selected as the exclusive sugar component of nucleic acids. The selection is explained by using molecular models and by eliminating most of the other common sugars by looking at their chemical structure and envisioning how they would fit in a nucleic acid model. Comparisons of sugar pucker conformations and configurations of pentoses indicate that ribose was not randomly selected but the only choice, since beta-D-ribose fits best into the structure of physiological forms of nucleic acids. In other nucleotides containing arabinose, xylose, or lyxose, the C(2)'-OH and/or the C(3)'-OH are above the furanose ring, causing steric interference with the bulky base and the C(5)'-OH group.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
kromatin szerkezet
Megjelenés:DNA and Cell Biology. - 25 : 5 (2006), p. 295-301. -
További szerzők:Szemán-Nagy Gábor (1975-) (biológia tanár-molekuláris biológus) Gácsi Mariann Röszer Tamás (1979-) (orvos, biológus) Basnakian, Alexei G.
Internet cím:elektronikus változat
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5.

001-es BibID:BIBFORM001286
Első szerző:Gácsi Mariann
Cím:Condensation of interphase chromatin in nuclei of synchronized chinese hamster ovary (CHO-K1) cells / Mariann Gacsi, Gabor Nagy, Gabor Pinter, Alexei G. Basnakian, Gaspar Banfalvi
Dátum:2005
Megjegyzések:Reversibly permeabilized cells have been used to visualize interphase chromatin structures in the presence and absence of biotinylated nucleotides. By reversing permeabilization, it was possible to confirm the existence of a flexible chromatin folding pattern through a series of transient geometric forms such as supercoiled, circular forms, chromatin bodies, thin and thick fibers, and elongated chromosomes. Our results show that the incorporation of biotin-11-dUTP interferes with chromatin condensation, leading to the accumulation of decondensed chromatin structures. Chromatin condensation without nucleotide incorporation was also studied in cell populations synchronized by centrifugal elutriation. After reversal of permeabilization, nuclei were isolated and chromatin structures were visualized after DAPI staining by fluorescent microscopy. Decondensed veil-like structures were observed in the early S phase (at an average C-value of 2.21), supercoiled chromatin later in the early S (2, 55 C), fibrous structures in the early mid S phase (2, 76 C), ribboned structures in the mid-S phase (2, 98 C), continuous chromatin strings later in the mid-S phase (3,28), elongated prechromosomes in the late S-phase (3, 72 C), precondensed chromosomes at the end and after the S phase (3, 99 C). Fluorescent microscopy revealed that neither interphase nor metaphase chromosomes are separate entities but form a linear array arranged in a semicircle. Linear arrangement was confirmed by computer image analysis.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
interfázisos kromatin
kromatin kondenzálás
elutriálás
permeábilis sejtek
biotinált DNA
Megjelenés:DNA and Cell Biology. - 24 : 1 (2005), p. 43-53. -
További szerzők:Szemán-Nagy Gábor (1975-) (biológia tanár-molekuláris biológus) Pintér Gábor Basnakian, Alexei G. Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész)
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6.

001-es BibID:BIBFORM001242
035-os BibID:(Wos)000235672300004 (Scopus)33344470488
Első szerző:Röszer Tamás (orvos, biológus)
Cím:The neuropeptide FMRFamide can protect cells against apoptosis in the snail digestive gland / T. Rőszer, J. Kappelmayer, G. G. Nagy, A. J. Szentmiklósi, A. G. Basnakian, G. Bánfalvi
Dátum:2006
Megjegyzések:FMRFamide-related peptides are widespread neurotransmitters or neurohormones regulating somatic or visceral motor activity. Some recent data indicate that these neuropeptides may be involved in the control of cell proliferation and apoptosis. In this work we investigated the possible effect of FMRFamide on cell viability in an invertebrate-type proliferating tissue. As a model, we used the midintestinal gland of the snail, Helix lucorum Linnaeus. Immunohistochemistry demonstrated the direct innervation of the gland cells by FMRFamide-containing nerve fibers. Midintestinal glands of snails were injected with 50 microM FMRFamide and the control with sterile deionised water or bovine serum albumin (BSA). Injections were administrated 4 times. Transmission electron microscopy, annexin V-labeling, thiazolyl blue (MTT) viability tests and ploidy analyses were carried out to define the viable/dead cell ratio in the tissue samples. FMRFamide increased the MTT-reduction of tissues, reduced the amount of apoptotic nuclei and annexin V-labeled cells. Deionised water or BSA injection induced cell death. Cell cycle analysis revealed that FMRFamide significantly elevated the amount of cells in G0/G1 phase, but did not induce mitosis. We conclude, that the FMRFamide can be a life-signal for cells, protect them from apoptosis without altering mitosis.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
antiapoptotikus neuropeptid
Megjelenés:Apoptosis. - 11 : 2 (2006), p. 173-182. -
További szerzők:Kappelmayer János (1960-) (laboratóriumi szakorvos) Szemán-Nagy Gábor (1975-) (biológia tanár-molekuláris biológus) Szentmiklósi József András (1948-) (farmakológus, klinikai laboratóriumi szakorvos) Basnakian, Alexei G. Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész)
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7.

001-es BibID:BIBFORM001308
Első szerző:Szemán-Nagy Gábor (biológia tanár-molekuláris biológus)
Cím:Gamma irradiation-induced apoptosis in murine pre-B cells prevents the condensation of fibrillar chromatin in early S phase / G. Nagy, M. Gacsi, M. Rehak, A. G. Basnakian, M. Klaisz, G. Banfalvi
Dátum:2004
ISSN:1360-8185
Megjegyzések:Local changes in chromatin structure leading to temporally distinct geometric forms were characterized in nuclei of reversibly permeabilized cells. Reversal of permeabilization was tested by 3H-thymidine incorporation and trypan blue dye exclusion. Apoptotic changes were visualized in a cell cycle dependent manner at the chromatin level by fluorescent microscopy in non-irradiated cells and after 400 rad Co60 irradiation. Fluorescent microscopy of chromatin structures belonging mainly to the interphase of the cell cycle confirmed the existence of specific geometric forms in nuclei of non-irradiated cells. In this control population, the following main transitory forms of condensing chromatin were distinguished: decondensed veil-like structures and fibrous structures in early and mid S phase (2.0-2.5 average C-value), chromatin bodies, semicircles later in mid S phase (3.0-3.5 C), precondensed chromosomes in late S (3.5-3.7 C) and metaphase chromosomes at the end and after S phase (3.7-4.0 C). Our results show that upon gamma-irradiation (a) the cellular and nuclear sizes were increased, (b) the DNA content was lower in each elutriated subpopulation of cells, (c) the progression of the cell cycle was arrested in the early S phase at 2.4 C value, (d) the chromatin condensation was blocked between the fibrillar chromatin and precondensed elongated chromosomal forms, and (e) the number and size of apoptotic bodies were inversely correlated with the progression of the cell cycle, with many small apoptotic bodies in early S phase and less and larger apoptotic bodies in late S phase.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
gamma sugárzás
kromatin kondenzálás
fibrilláris kromatin
Megjelenés:Apoptosis. - 9 : 6 (2004), p. 765-776. -
További szerzők:Gácsi Mariann Rehák Mariann Basnakian, Alexei G. Klaisz Mariann Bánfalvi Gáspár (1943-) (sejtbiológus, gyógyszerész)
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