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001-es BibID:	BIBFORM001280
Első szerző:	Bánfalvi Gáspár (sejtbiológus, gyógyszerész)
Cím:	Cell culture density dependent toxicity and chromatin changes upon cadmium treatment in murine pre-B-cells / Gaspar Banfalvi, Kinga Ujvarosi, Gyorgy Trencsenyi, Csilla Somogyi, Gabor Nagy, Alexei Basnakian
Dátum:	2007
Megjegyzések:	Murine pre-B-cells grown in the presence of lower (1 microM) or higher (5 microM) concentration of cadmium chloride were separated into 13 fractions by centrifugal elutriation. The rate of DNA synthesis after cadmium treatment determined in permeable cells was dependent on cell culture density during cadmium treatment. Cell cycle analysis revealed a shift in the profile of DNA synthesis from replicative to repair DNA synthesis upon cadmium treatment. The study of the relationship between cell culture density and cell diameter at lower and higher cell densities in the presence of 1 microM cadmium chloride concentration showed that a. at 5 x 10(5) cell/ml or lower densities cells were shrinking indicating apoptotic changes, b. at higher cell culture densities the average cell size increased, c. the treatment of cells with low CdCl(2) concentration (1 microM) at higher cell culture density (>5 x 10(5) cell/ml) did not change significantly the average cell diameter. At 5 microM cadmium concentration and higher cell culture densities (>5 x 10(5) cell/ml) the average cell size decreased in each elutriated fraction. Most significant inhibition of cell growth took place in early S phase (2.0-2.5 C value). Apoptotic chromatin changes in chromatin structure after cadmium treatment were seen as large extensive disruptions, holes in the nuclear membrane and stickiness of incompletely folded chromosomes.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban kadmium toxicitás kromatin szerkezet változás reverzibilis premeabilizálás sejt szinkronizálás
Megjelenés:	Apoptosis. - 12 : 7 (2007), p. 1219-1228. -
További szerzők:	Ujvárosi Kinga Trencsényi György (1978-) (biológus, biokémikus, molekuláris biológus) Somogyi Csilla (1983-) (biológus, angol-magyar szakfordító) Szemán-Nagy Gábor (1975-) (biológia tanár-molekuláris biológus) Basnakian, Alexei G.
Internet cím:	elektronikus változat DOI
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001-es BibID:	BIBFORM001278
Első szerző:	Bánfalvi Gáspár (sejtbiológus, gyógyszerész)
Cím:	Gamma irradiation induced apoptotic changes in the chromatin structure of human erythroleukemia K562 cells / Gaspar Banfalvi, Mariann Klaisz, Kinga Ujvarosi, Gyorgy Trencsenyi, David Rozsa, Gabor Nagy
Dátum:	2007
Megjegyzések:	Exponentially growing human erythroleukemia K562 cells were synchronized by centrifugal elutriation prior to and after Co60 gamma-irradiation (4 Gy). Forward scatter flow cytometry used for size analysis revealed the increase of an early apoptotic cell population ranging from lower (0.05 C-value) to higher DNA content (approximately 1 C) as the cells progressed through the S phase. The increase in cellular DNA content expressed in C-values correlated with apoptotic chromatin changes manifested as many small apoptotic bodies in early S phase and larger but less numerous disintegrated apoptotic bodies in late S phase. Most significant changes after exposure to gamma-irradiation took place in early S phase resulting in an increase of nuclear size by more than 50%. Cell fractions containing irradiated cells showed enhanced growth arrest at 2.4 C-value, which was accompanied by apoptosis. Apoptotic cell cycle arrest near to the G1/G0 checkpoint and apoptotic changes indicate that the radiation resistance of K562 cells is related to the bypass of the early stage of the p53 apoptotic pathway. Apoptotic changes in chromatin structure induced by gamma-irradiation indicate that these injury-specific changes can be identified and distinguished from chromatin changes induced by UV radiation or heavy metals.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban gamma sugárzás apoptózis kromatin szerkezet
Megjelenés:	Apoptosis. - 12 : 12 (2007), p. 2271-2283. -
További szerzők:	Klaisz Mariann Ujvárosi Kinga Trencsényi György (1978-) (biológus, biokémikus, molekuláris biológus) Rózsa Dávid (1982-) (Ph.D hallgató) Szemán-Nagy Gábor (1975-) (biológia tanár-molekuláris biológus)
Internet cím:	elektronikus változat DOI
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