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1.
001-es BibID:
BIBFORM008901
Első szerző:
Magyarné Tábori Katalin (kertészmérnök)
Cím:
The role of cytokinins in shoot organogenesis in apple / Katalin Magyar-Tábori, Judit Dobránszki, Jaime A. Teixeira da Silva, Sean M. Bulley, Ildikó Hudák
Dátum:
2010
ISSN:
0167-6857
Tárgyszavak:
Agrártudományok
Növénytermesztési és kertészeti tudományok
idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:
Plant Cell, Tissue and Organ Culture. - 101 : 3 (2010), p. 251-267. -
További szerzők:
Dobránszki Judit (1967-) (biológus)
Teixeira da Silva, Jaime A.
Bulley, Sean M.
Hudák Ildikó (1978-) (biológia környezettan tanár)
Internet cím:
DOI
elektronikus változat
Borító:
Saját polcon:
2.
001-es BibID:
BIBFORM089761
Első szerző:
Teixeira da Silva, Jaime A.
Cím:
Genetic transformation and molecular research in Anthurium: progress and prospects / Jaime A. Teixeira da Silva, Judit Dobránszki, Songjun Zeng, Budi Winarto, Adrian M. Lennon, Yasmina Jaufeerally-Fakim, David A. Christopher
Dátum:
2015
ISSN:
0167-6857
Megjegyzések:
Although Anthurium is an attractive and commercially popular ornamental plant, its genetic enhancement has lagged behind that of other ornamental crops. There are several agronomically important traits in need of improvement. These include novel flower colors and morphologies, increased shelf and vase lives, and resistance to bacterial blight (Xanthomonas axonopodis pv. dieffenbachiae), burrowing nematodes and abiotic stresses. The production of transgenic Anthuriums is critical because the conventional breeding of a cultivar with beneficial traits typically requires 8-10 years. This review evaluates the problems, challenges and progress associated with developing molecular markers for Anthurium and in genetically transforming this ornamental. Recent improvements have hastened the tissue culture and regeneration of transgenic plants primarily using Agrobacterium-based methods. Promoter analyses have focused on constitutive and tissue-enhanced gene expression with the green fluorescent protein being a more reliable reporter than ?-glucuronidase. The development of molecular markers assists with phylogenetic analyses and PCR-based markers such as RAPD, SSR, SPAR, ISSR and AFLP can be used to differentiate cultivars and for genetic fingerprinting. The marker-assisted breeding of Anthurium will become more feasible once available data are used for association to specific traits. Work on the identification of quantitative trait loci for disease resistance and other traits such as flower colour is required and should incorporate new approaches, such as next-generation sequencing technologies. By highlighting the aforementioned bottlenecks and successes in this review, it is expected that the pace of Anthurium genetic improvement will increase with the multifaceted incorporation of focused priorities and new technology advancements.
Tárgyszavak:
Természettudományok
Biológiai tudományok
idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:
Plant Cell Tissue And Organ Culture. - 123 : 2 (2015), p. 205-219. -
További szerzők:
Dobránszki Judit (1967-) (biológus)
Zeng, Song-Jun (agrár)
Winarto, Budi (agrár)
Lennon, Adrian M.
Jaufeerally-Fakim, Yasmina
Christopher, David A.
Internet cím:
Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:
3.
001-es BibID:
BIBFORM078734
Első szerző:
Teixeira da Silva, Jaime A.
Cím:
mRNA transcription profile of potato (Solanum tuberosum L.) in response to explant cutting / Jaime A. Teixeira da Silva, Hidvégi Norbert, Gulyás Andrea, Dobránszki Judit
Dátum:
2019
Megjegyzések:
The explant is an important parameter in plant tissue culture. Most plant tissue culture studies in the literature have focused almost exclusively on the use of explants as a tool for organogenesis or (micro)propagation, and there is limited information that documents the changes that occur in explants in response to cutting during explant preparation just prior to or during culture. To better understand the molecular changes occurring during the wounding process that accompanies the preparation of an explant, this study examined the mRNA transcription profile of 4-week-old potato (Solanum tuberosum L. cv. Desirée) single-node stem segments. To achieve this, RNA-seq analysis was performed to identify significantly differentially expressed genes (DEGs) that existed between uncut in vitro plantlets and cut explants. DEGs corresponding to specific enzyme profiles were broadly connected to cellular processes in the extracellular region, nucleus, and plasma membrane, and were associated with biosynthesis, carbohydrate metabolism and catabolism, cellular protein modification, growth and development, and response to stress. The RNA-seq data of eight highly up- or down-regulated DEGs was validated by RT-qPCR, showing high positive Spearman and Pearson correlation coefficients (0.73 and 0.95, respectively) between SeqMonk LFC and RT-qPCR LFC. These results provide a novel perspective of the changes taking place within explants when they are cut and serve as a valuable basis for the study of explant-related stress in plant tissue culture in other species. In addition, our findings may contribute to an understanding of the success or failure of the tissue culture protocol and subsequent establishment of in vitro cultures by better appreciating the stress-related molecular changes taking place.
Tárgyszavak:
Természettudományok
Biológiai tudományok
idegen nyelvű folyóiratközlemény külföldi lapban
Abiotic stress
Antioxidants
Development
Plant growth
Plant tissue culture
Megjelenés:
Plant Cell, Tissue and Organ Culture. - 138 : 1 (2019), p. 143-152. -
További szerzők:
Hidvégi Norbert (1986-)
Gulyás Andrea (1985-)
Dobránszki Judit (1967-) (biológus)
Pályázati támogatás:
20428-3/2018/ FEKUTSTRAT
FIKP
Internet cím:
Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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