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001-es BibID:	BIBFORM102892
035-os BibID:	(cikkazonosító)677 (WoS)000846075500001 (Scopus)85137460294
Első szerző:	Abdalla, Neama
Cím:	An Academic and Technical Overview on Plant Micropropagation Challenges / Neama Abdalla, Hassan El-Ramady, Mayada K. Seliem, Mohammed E. El-Mahrouk, Naglaa Taha, Yousry Bayoumi, Tarek A. Shalaby, Judit Dobránszki
Dátum:	2022
ISSN:	2311-7524
Megjegyzések:	The production of micropropagated plants in plant-tissue-culture laboratories and nurseries is the most important method for propagation of many economic plants. Micropropagation based on tissue-culture technology involves large-scale propagation, as it allows multiplication of a huge number of true-to-type propagules in a very short time and in a very limited space, as well as all year round, regardless of the climate. However, applying plant-tissue-culture techniques for the commercial propagation of plants may face a lot of obstacles or troubles that could result from technical, biological, physiological, and/or genetical reasons, or due to overproduction or the lack of facilities and professional technicians, as shown in the current study. Moreover, several disorders and abnormalities are discussed in the present review. This study aims to show the most serious problems and obstacles of plant micropropagation, and their solutions from both scientific and technical sides. This review, as a first report, includes different challenges in plant micropropagation (i.e., contamination, delay of subculture, burned plantlets, browning, in vitro rooting difficulty, somaclonal variations, hyperhydricity, shoot tip necrosis, albino plantlets, recalcitrance, shoot abnormalities, in vitro habituation) in one paper. Most of these problems are related to scientific and/or technical reasons, and they could be avoided by following the micropropagation protocol suitable for each plant species. The others are dominant in plant-tissue-culture laboratories, in which facilities are often incomplete, or due to poor infrastructure and scarce funds.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk acclimatization browning contamination delay of subculture hyperhydricity recalcitrance somaclonal variations totipotency
Megjelenés:	Horticulturae. - 8 : 8 (2022), p. 1-28. -
További szerzők:	El-Ramady, Hassan (1969-) (agrármérnök) Seliem, Mayada Kadri Elmahrouk, Mohammed Taha, Naglaa Bayoumi, Yousry Shalaby, Tarek Dobránszki Judit (1967-) (biológus)
Pályázati támogatás:	TKP2021-EGA-20 Egyéb
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001-es BibID:	BIBFORM115406
035-os BibID:	(cikkazonosító)2633 (WoS)001098265000001 (Scopus)85175375665
Első szerző:	Higazy, Ahmed E.
Cím:	Production of Black Cumin via Somatic Embryogenesis, Chemical Profile of Active Compounds in Callus Cultures and Somatic Embryos at Different Auxin Supplementations / Ahmed E. Higazy, Mohammed E. El-Mahrouk, Antar N. El-Banna, Mosaad K. Maamoun, Hassan El-Ramady, Neama Abdalla, Judit Dobránszki
Dátum:	2023
ISSN:	2073-4395
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk black cumin Ranunculaceae medicinal plants in vitro cultures auxins phytochemicals gas chromatography thymoquinone antibacterial anticancer antioxidant
Megjelenés:	Agronomy-Basel. - 13 : 10 (2023), p. 1-17. -
További szerzők:	Elmahrouk, Mohammed El-Banna, Antar N. Maamoun, Mosaad K. El-Ramady, Hassan (1969-) (agrármérnök) Abdalla, Neama Dobránszki Judit (1967-) (biológus)
Pályázati támogatás:	TKP2021-EGA-20 FIKP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM106325
035-os BibID:	(cikkazonosító)306 (Scopus)85146764492
Első szerző:	Mohamed, Suzan M.
Cím:	Optimizing Medium Composition and Environmental Culture Condition Enhances Antioxidant Enzymes, Recovers Gypsophila paniculata L. Hyperhydric Shoots and Improves Rooting In Vitro / Suzan M. Mohamed, Mohammed E. El-Mahrouk, Antar N. El-Banna, Yaser M. Hafez, Hassan El-Ramady, Neama Abdalla, Judit Dobránszki
Dátum:	2023
ISSN:	2223-7747
Megjegyzések:	: Gypsophila paniculata L. is one of the most important commercial cut flowers worldwide. The plant is sterile and propagated mainly by in vitro culture techniques. However, hyperhydricity hinders its micropropagation and increases mortality during ex vitro acclimatization. Hyperhydric shoots of G. paniculata were proliferated from nodal explants on MS medium without growth regulators that contained 30 g L?1 sucrose, and gelled with 6.0 g L1 agar. Medium components and environmental culture conditions were optimized to revert hyperhydricity in G. paniculata microshoots and develop an efficient micropropagation protocol for commercial production. Multiple shoots with high quality were successfully regenerated on MS medium fortified with potassium and ammonium nitrate at full concentration, 2.0 mg L?1 paclobutrazol, solidified with 9.0 g L?1agar in Magenta boxes of 62.87 gas exchange/day and incubated under light density of 60 ?mol m?2 s ?1 . We recorded 4.33 shoots, 40.00 leaves, 6.33 cm, 2.50 g and 95.00% for number of shoots/explant, number of leaves/shoot, shoot length, shoot fresh weight and normal shoots percentage, respectively. Well-rooted plantlets of G. paniculata were developed from the reverted microshoots, with the rooting percentage (95.00%) on MS medium augmented with 1.0 mg L?1 IBA in Magenta boxes of 62.87 gas exchange/day and 60 ?mol m?2 s ?1 light density. In vitro-rooted plantlets exhibited reduced electrolyte leakage, and enhanced antioxidant enzymes activity of peroxidase, catalase, and polyphenol oxidase due to good ventilation at the highest gas exchange rate of the culture vessels.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk paclobutrazol gas exchange rates light density catalase peroxidase polyphenol oxidase electrolyte leakage
Megjelenés:	Plants-Basel. - 12 : 2 (2023), 306. -p. 1-17. -
További szerzők:	Elmahrouk, Mohammed El-Banna, Antar N. Hafez, Yaser M. El-Ramady, Hassan (1969-) (agrármérnök) Abdalla, Neama Dobránszki Judit (1967-) (biológus)
Pályázati támogatás:	TKP2021-EGA FIKP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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