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001-es BibID:BIBFORM106134
035-os BibID:(cikkazonosító)22348 (WOS)000904416000001 (Scopus)85144635665
Első szerző:Astuti, Putri Kusuma (állattenyésztési genetikus)
Cím:Validation of SNP markers for thermotolerance adaptation in Ovis aries adapted to different climatic regions using KASP-PCR technique / Putri Kusuma Astuti, Daniela Elena Ilie, Dinu Gavojdian, George Wanjala, Bouabid Badaoui, Husein Ohran, Eva Pasic-Juhas, Zoltán Bagi, András Jávor, Szilvia Kusza
Dátum:2022
ISSN:2045-2322
Megjegyzések:A study on 51 SNPs belonging to 29 genes related to heat stress was carried out in 720 sheep from 17 different breeds adapted to different climates from Hungary, Bosnia and Herzegovina, Morocco and Romania, using Kompetitive Allele?Specific Polymerase Chain Reaction. Genotype frequency and the Hardy?Weinberg equilibrium were calculated, followed by a clustering using the Principal Component Analysis. We analyzed the polymorphisms in the following genes analyzed: HSPA12A, HSP90AA1, IL33, DIO2, BTNL2, CSN2, ABCG1, CSN1S1, GHR, HSPA8, STAT3, and HCRT. We emphasized on HSPA12A and HSPA8 genes as they were successfully genotyped in all studied flocks in which genotype frequency patterns were identified. Contrary to previous findings, the A allele for HSPA8 SNP was not observed in the heat tolerant breeds, being found exclusively in cold-tolerant breeds. The principal component analysis could not clearly differentiate the breeds, while plot concentration was slightly varied among the three groups, with HSP90AA1 and IL33 SNPs' loading values significantly contributing to PC1 and PC2. We confirmed previous works that the HSPA12A, HSPA8, HSP90AA1 and IL33 SNPs are potential candidate markers for thermotolerance adaptation in sheep. This research contributes to the genetic variability of SNPs for thermotolerance adaptability in sheep.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Scientific Reports. - 12 (2022), p. 1-9. -
További szerzők:Ilie, Daniela Elena Gavojdian, Dinu Wanjala, George (1984-) (Animal scientist) Badaoui, Bouabid (1979-) (animal genetics engineer) Ohran, Husein Pasic-Juhas, Eva Bagi Zoltán (1987-) (természetvédelmi mérnök, állatgenetika) Jávor András (1952-) (agrármérnök) Kusza Szilvia (1979-) (agrármérnök)
Pályázati támogatás:EFOP-3.6.2-16-2017-00001
EFOP
2021-1.2.4-TÉT-2021-00014
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001-es BibID:BIBFORM061520
035-os BibID:(WoS)000371800000009 (Scopus)84960539521
Első szerző:Csikós Ádám (molekuláris biológus)
Cím:Applicability and sensitivity of PCR-SSCP method for milk species identification in cheese / A. Csikós, A. Hodzic, E. Pasic-Juhas, A. Jávor, A. Hrkovic-Porobija, T. Goletic, G. Gulyás, L. Czeglédi
Dátum:2016
ISSN:0139-3006 1588-2535
Megjegyzések:Species identification in food has become a prominent issue in recent years as the importance of consumer protection has increased. DNA-based species identification methods were developed by researchers in the last two decades, as these are reliable, accurate, and low-cost techniques for species identification in raw and processed food products as well. In our study, universal primers were designed to conserved regions of mitochondrial 12S rRNA. Amplicons were heat-denatured and a PCR single strand conformation polymorphism (SSCP) method was developed to identify cattle, buffalo, sheep, and goat DNA. Sensitivity of this technique was tested on DNA mixtures of cattle-sheep, cattle-goat, and cattle-buffalo and the threshold limit of cattle DNA was 5%, 5%, and 3%, respectively. One hundred and five cheeses were purchased and collected from Bosnian and Hungarian farmers, retails, and supermarkets to reveal fraud, 32 percent of them (34 cheeses) were found to be mislabelled by species.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény hazai lapban
folyóiratcikk
Megjelenés:Acta Alimentaria. - 45 : 1 (2016), p. 69-76. -
További szerzők:Hodzic, Aida Pasic-Juhas, Eva Jávor András (1952-) (agrármérnök) Hrkovic-Porobija, Amina Goletic, Teufik Gulyás Gabriella (1985-) (agrár) Czeglédi Levente (1977-) (agrármérnök)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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