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1.

001-es BibID:	BIBFORM076618
035-os BibID:	(cikkazonosító)e0204653 (WOS)000446383500033 (Scopus)85054465081
Első szerző:	Ashrafzadeh, Mohammad Reza
Cím:	Large-scale mitochondrial DNA analysis reveals new light on the phylogeography of Central and Eastern-European Brown hare (Lepus europaeus Pallas, 1778) / Mohammad Reza Ashrafzadeh, Mihajla Djan, László Szendrei, Algimantas Paulauskas, Massimo Scandura, Zoltán Bagi, Daniela Elena Ilie, Nikoloz Kerdikoshvili, Panek Marek, Noémi Soós, Szilvia Kusza
Dátum:	2018
ISSN:	1932-6203
Megjegyzések:	European brown hare, Lepus europaeus, from Central and Eastern European countries (Hungary, Poland, Serbia, Lithuania, Romania, Georgia and Italy) were sampled, and phylogenetic analyses were carried out on two datasets: 1.) 137 sequences (358 bp) of control region mtDNA; and 2.) 105 sequences of a concatenated fragment (916 bp), including the cytochrome b, tRNA-Thr, tRNA-Pro and control region mitochondrial DNA. Our sequences were aligned with additional brown hare sequences from GenBank. A total of 52 and 51 haplotypes were detected within the two datasets, respectively, and assigned to two previously described major lineages: Anatolian/Middle Eastern (AME) and European (EUR). Furthermore, the European lineage was divided into two subclades including South Eastern European (SEE) and Central European (CE). Sympatric distribution of the lineages of the brown hare in South-Eastern and Eastern Europe revealed contact zones there. BAPS analysis assigned sequences from L. europaeus to five genetic clusters, whereas CE individuals were assigned to only one cluster, and AME and SEE sequences were each assigned to two clusters. Our findings uncover numerous novel haplotypes of Anatolian/Middle Eastern brown hare outside their main range, as evidence for the combined influence of Late Pleistocene climatic fluctuations and anthropogenic activities in shaping the phylogeographic structure of the species. Our results support the hypothesis of a postglacial brown hare expansion from Anatolia and the Balkan Peninsula to Central and Eastern Europe, and suggest some slight introgression of individual haplotypes from L. timidus to L. europaeus.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Plos One. - 13 : 10 (2018), p. 1-20. -
További szerzők:	Djan, Mihajla (biológus) Szendrei László (1964-) (agrármérnök) Paulauskas, Algimantas Scandura, Massimo Bagi Zoltán (1987-) (természetvédelmi mérnök, állatgenetika) Ilie, Daniela Elena Kerdikoshvili, Nikoloz Marek, Panek Soós Noémi (1981-) (biológus) Kusza Szilvia (1979-) (agrármérnök)
Pályázati támogatás:	EFOP-3.6.3-VEKOP-16-2017-00008 EFOP
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2.

001-es BibID:	BIBFORM111442
035-os BibID:	(cikkazonosító)197 (Scopus)85158924988 (WoS)000983708900001
Első szerző:	Astuti, Putri Kusuma (állattenyésztési genetikus)
Cím:	Genetic polymorphism in European and African sheep breeds reared in Hungary based on 48 SNPs associated with resistance to gastrointestinal parasite infection using KASP-PCR technique / Putri Kusuma Astuti; Dinu Gavojdian; Daniela Elena Ilie; George Wanjala; István Monori; Zoltán Bagi; Szilvia Kusza
Dátum:	2023
ISSN:	0049-4747 1573-7438
Megjegyzések:	This pilot study used an alternative and economically efcient technique, the Kompetitive Allele-Specifc Polymerase Chain Reaction (KASP-PCR) to examine 48 SNPs from 11 parasite-resistance genes found on 8 chromosomes in 110 animals from fve sheep breeds reared in Hungary; Hungarian Tsigai, White Dorper, Dorper, Ile de France, and Hungarian Merino. Allele and genotype frequencies, fxation index, observed heterozygosity, expected heterozygosity, F statistic, and their relationship with the Hardy-Weinberg equilibrium (WHE) and the polymorphic information content (PIC) were determined, followed by principal component analysis (PCA). As much as 32 SNPs out of the 48 initially studied were successfully genotyped. A total of 9 SNPs, 4 SNPs in TLR5, 1 SNP in TLR8, and 4 SNPs in TLR2 genes, were polymorphic. The variable genotype and allele frequency of the TLRs gene indicated genetic variability among the studied sheep breeds, with the Hungarian Merino exhibiting the most polymorphisms, while Dorper was the population with the most SNPs departing from the HWE. According to the PIC value, the rs430457884-TLR2, rs55631273-TLR2, and rs416833129-TLR5 were found to be informative in detecting polymorphisms among individuals within the populations, whereas the rs429546187-TLR5 and rs424975389- TLR5 were found to have a signifcant infuence in clustering the population studied. This study reported a moderate level of genetic variability and that a low to moderate within-breed diversity was maintained in the studied populations.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk KASP PCR Ovis aries Parasite resistance genes Polymorphism TLRs
Megjelenés:	Tropical Animal Health and Production. - 55 : 3 (2023), p. 1-12. -
További szerzők:	Gavojdian, Dinu Ilie, Daniela Elena Wanjala, George (1984-) (Animal scientist) Monori István (1976-) (agrármérnök) Bagi Zoltán (1987-) (természetvédelmi mérnök, állatgenetika) Kusza Szilvia (1979-) (agrármérnök)
Pályázati támogatás:	EFOP-3.6.2-16-2017-00001 EFOP VP3-16.1.1-4.1.5- 4.2.1-4.2.2-8.1.1-8.2.1-8.3.1-8.5.1-8.5.2-8.6.1-17 Egyéb
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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3.

001-es BibID:	BIBFORM106134
035-os BibID:	(cikkazonosító)22348 (WOS)000904416000001 (Scopus)85144635665
Első szerző:	Astuti, Putri Kusuma (állattenyésztési genetikus)
Cím:	Validation of SNP markers for thermotolerance adaptation in Ovis aries adapted to different climatic regions using KASP-PCR technique / Putri Kusuma Astuti, Daniela Elena Ilie, Dinu Gavojdian, George Wanjala, Bouabid Badaoui, Husein Ohran, Eva Pasic-Juhas, Zoltán Bagi, András Jávor, Szilvia Kusza
Dátum:	2022
ISSN:	2045-2322
Megjegyzések:	A study on 51 SNPs belonging to 29 genes related to heat stress was carried out in 720 sheep from 17 different breeds adapted to different climates from Hungary, Bosnia and Herzegovina, Morocco and Romania, using Kompetitive Allele?Specific Polymerase Chain Reaction. Genotype frequency and the Hardy?Weinberg equilibrium were calculated, followed by a clustering using the Principal Component Analysis. We analyzed the polymorphisms in the following genes analyzed: HSPA12A, HSP90AA1, IL33, DIO2, BTNL2, CSN2, ABCG1, CSN1S1, GHR, HSPA8, STAT3, and HCRT. We emphasized on HSPA12A and HSPA8 genes as they were successfully genotyped in all studied flocks in which genotype frequency patterns were identified. Contrary to previous findings, the A allele for HSPA8 SNP was not observed in the heat tolerant breeds, being found exclusively in cold-tolerant breeds. The principal component analysis could not clearly differentiate the breeds, while plot concentration was slightly varied among the three groups, with HSP90AA1 and IL33 SNPs' loading values significantly contributing to PC1 and PC2. We confirmed previous works that the HSPA12A, HSPA8, HSP90AA1 and IL33 SNPs are potential candidate markers for thermotolerance adaptation in sheep. This research contributes to the genetic variability of SNPs for thermotolerance adaptability in sheep.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Scientific Reports. - 12 (2022), p. 1-9. -
További szerzők:	Ilie, Daniela Elena Gavojdian, Dinu Wanjala, George (1984-) (Animal scientist) Badaoui, Bouabid (1979-) (animal genetics engineer) Ohran, Husein Pasic-Juhas, Eva Bagi Zoltán (1987-) (természetvédelmi mérnök, állatgenetika) Jávor András (1952-) (agrármérnök) Kusza Szilvia (1979-) (agrármérnök)
Pályázati támogatás:	EFOP-3.6.2-16-2017-00001 EFOP 2021-1.2.4-TÉT-2021-00014 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:	BIBFORM087990
Első szerző:	Cziszter, Ludovic Toma
Cím:	Effects of temperament on production and reproductive performances in Simmental dual-purpose cows / Cziszter Ludovic Toma, Gavojdian Dinu, Neamt Radu, Neciu Florin, Kusza Szilvia, Ilie Daniela-Elena
Dátum:	2016
ISSN:	1558-7878
Megjegyzések:	The aim of the current research was to evaluate the effects that temperament has on production and reproduction performances in Simmental dual-purpose cattle breed. Behavioral reactivity of cows significantly influenced (P <= 0.05) the body weight, milk yield, fat yield, protein yield, protein content, and the calving interval of the cows included in the study-head, having more substantial effects (P <= 0.001) on the milking speed and the number of steps per day, with calmer cows outperforming the nervous counterparts. However, temperament did not influence (P > 0.05) traits such as days open, number of inseminations per gestation, fat percentage, somatic cell count, body condition score, cleanliness of udder and cleanliness of hindquarter. Significant negative phenotypic correlations were found between temperament and cows body weight (-0.19), milk yield (-0.19), fat yield (-0.14), protein yield (-0.18), and milking speed (-0.18). Current results suggest that selection for calm temperaments will translate into increased milk, fat, and protein yields in Simmental cattle, as well as shorter the calving interval and improved milk ejection.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk animal welfare behavioral reactivity cattle Simmental selection traits
Megjelenés:	Journal of Veterinary Behavior. - 15 (2016), p. 50-55. -
További szerzők:	Gavojdian, Dinu Neamt, Radu Neciu, Florin Kusza Szilvia (1979-) (agrármérnök) Ilie, Daniela Elena
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:	BIBFORM111042
035-os BibID:	(cikkazonosító)1484 (WoS)000987004100001 (Scopus)85159145210
Első szerző:	Ilie, Daniela Elena
Cím:	Kompetitive Allele Specific PCR Genotyping of 89 SNPs in Romanian Spotted and Romanian Brown Cattle Breeds and Their Association with Clinical Mastitis / Daniela Elena Ilie; Dinu Gavojdian; Szilvia Kusza; Radu Ionel Neamț; Alexandru Eugeniu Mizeranschi; Ciprian Valentin Mihali; Ludovic Toma Cziszter
Dátum:	2023
ISSN:	2076-2615
Megjegyzések:	Mastitis is the most common production disease in the dairy sector worldwide, its incidence being associated with both cows` exposure to bacteria and the cows` genetic make-up for resistance to pathogens. The objective of our study was to analyse 89 missense SNPs belonging to six genes (CXCR2, CXCL8, TLR4, BRCA1, LTF, BOLA-DRB3), which were found to be associated with genetic resistance or susceptibility to mastitis. A total of 298 cattle (250 Romanian Spotted and 48 Romanian Brown) were genotyped by Kompetitive Allele Specific PCR (KASP) and a chi-squared test was used for genetic association studies with clinical mastitis. A total of 35 SNPs (39.3%) among the selected 89 SNPs were successfully genotyped, of which 31 markers were monomorphic. The polymorphic markers were found in two genes: TLR4 (rs460053411) and BOLA-DRB3 (rs42309897, rs208816121, rs110124025). The polymorphic SNPs with MAF > 5% and call rates > 95% were used for the association study. The results showed that rs110124025 in the BOLA-DRB3 gene was significantly associated with mastitis prevalence (p ? 0.05) in both investigated breeds. Current results show that the SNP rs110124025 in the BOLA-DRB3 gene can be used as a candidate genetic marker in selection for mastitis resistance in Romanian dairy cattle.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk KASP mastitis polymorphism Romanian Brown Romanian Spotted
Megjelenés:	Animals. - 13 : 9 (2023), p. 1-13. -
További szerzők:	Gavojdian, Dinu Kusza Szilvia (1979-) (agrármérnök) Neamt, Radu Mizeranschi, Alexandru Eugeniu Ciprian, Valentin Mihali Cziszter, Ludovic Toma
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:	BIBFORM088166
035-os BibID:	(cikkazonosító)e013873
Első szerző:	Ilie, Daniela Elena
Cím:	Microsatellite and Mitochondrial DNA Study of Native Eastern European Cattle Populations: The Case of the Romanian Grey / Ilie Daniela Elena, Cean Ada, Cziszter Ludovic Toma, Gavojdian Dinu, Iván Alexandra, Kusza Szilvia
Dátum:	2015
ISSN:	1932-6203 1932-6203
Megjegyzések:	The Eastern European Grey cattle are regarded as the direct descendants of the aurochs(Bos taurus primigenius). Nowadays in Romania, less than 100 Grey animals are being reared and included in the national gene reserve. We examined the genetic diversity among Romanian Grey, Brown, Spotted and Black and White cattle breeds, with a particular focuson Romanian Grey through the use of (i) 11 bovine specific microsatellite markers on 83 animals and (ii) 638 bp length of mitochondrial DNA (mtDNA) D-loop region sequence data from a total of 81 animals. Both microsatellite and mtDNA analysis revealed a high level of genetic variation in the studied breeds. In Romanian Grey a total of 100 alleles were found,the mean number of observed alleles per locus was 9.091; the average observed heterozygosity was 0.940; the Wright's fixation index (FIS) was negative (-0.189) and indicates that there is no inbreeding and no selection pressure. MtDNA analysis revealed 52 haplotypes with 67 variable sites among the Romanian cattle breeds without any insertion or deletion.Haplotype diversity was 0.980?0.007 and ranged from 0.883?0.056 (Brown) to 0.990?0.028 (Spotted and Black and White). The highest genetic variability of the mtDNA was recorded in the Grey breed, where 18 haplotypes were identified. The most frequent mtDNAD-loop region belonged to T3 haplogroup (80.247%), which was found across all studied breeds, while T2 haplotypes (16.049%) was only found in Grey, Spotted and Black and White genotypes. The T1 haplotypes (3.704%) were found in the Grey and Spotted. The current results contribute to the general knowledge on genetic diversity found in Eastern European cattle breeds and could prove a valuable tool for the conservation efforts of animal genetic resources (FAnGR).
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk DNA Study Native Eastern European Cattle Populations Romanian Grey
Megjelenés:	Plos One. - 10 : 9 (2015), p. 1-18. -
További szerzők:	Cean, Ada Cziszter, Ludovic Toma Gavojdian, Dinu Iván Alexandra Kusza Szilvia (1979-) (agrármérnök)
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7.

001-es BibID:	BIBFORM079295
035-os BibID:	(cikkazonosító)e0197051 (WOS)000431757400057 (Scopus)85046772044
Első szerző:	Ilie, Daniela Elena
Cím:	Genetic characterization of indigenous goat breeds in Romania and Hungary with a special focus on genetic resistance to mastitis and gastrointestinal parasitism based on 40 SNPs / Daniela Elena Ilie, Kusza Szilvia, Maria Sauer, Dinu Gavojdian
Dátum:	2018
ISSN:	1932-6203
Megjegyzések:	Goat breeding has become an important sector in Eastern Europe, with Romania and Hungary being among the major producer countries. Given the limited number of research done up-to-date concerning genetic studies of indigenous goat breeds reared in Romania and Hungary, the current preliminary study aimed to analyze the variability of genes related to mastitis and gastrointestinal parasitism by using Kompetitive Allele Specific PCR (KASP?). We studied 52 single nucleotide polymorphisms (SNPs) belonging to 19 genes in indigenous breeds from both countries, namely Banat's White (n = 36), Carpatina (n = 35) from Romania and Hungarian Milking (n = 79) and identified 16 polymorphic SNPs among 10 genes (PTX3, IL6, CLEC4E, IL8, IL1RN, IL15RA, TNFSF13, SOCS3, TNF and TLR3) in 150 animals. Furthermore, the diversity of the studied breeds was investigated. The PIC values ranged from 0.042 to 0.691. The mean values of observed and expected heterozygosity were 0.235 and 0.246 respectively. The highest observed heterozygosity was obtained for IL15RA g.10343904C>T in Banat's White (0.464), IL15RA g.10354813C>T in Carpatina (0.577) and SOCS3 g.52626440T>G in Hungarian Milking (0.588). Pairwise FST values between the Romanian breeds and Romanian and Hungarian breeds were small (0.009 and 0.015), indicating the close relationship among the studied goat populations. From all the polymorphic SNPs identified, the Hungarian Milking breed showed the highest proportion of polymorphisms (100%), whereas the Carpatina breed had the lowest percentage (87.5%). The highest value of MAF was obtained for SOCS3 g.52626440T>G (0.46), IL15RA g.10343904C>T (0.47), IL15RA g.10344025C>T (0.45), and IL15RA g.10354813C>T (0.42). The 16 polymorphic SNPs identified in a panel of 150 unrelated individuals belonging to three Romanian and Hungarian indigenous goat breeds could be used in future genomic based breeding schemes as markers for genetic resistance to mastitis and gastrointestinal parasitism in goat breeds found in Eastern and Central Europe.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Plos One. - 13 : 5 (2018), p. 1-14. -
További szerzők:	Kusza Szilvia (1979-) (agrármérnök) Sauer, Maria Gavojdian, Dinu
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8.

001-es BibID:	BIBFORM087951
Első szerző:	Kusza Szilvia (agrármérnök)
Cím:	Genetic polymorphism of CSN2 gene in Banat White and Carpatina goats / Kusza Szilvia, Ilie Daniela Elena, Sauer Maria, Nagy Krisztina, Patras Irina, Gavojdian Dinu
Dátum:	2016
ISSN:	0001-527X 1734-154X
Megjegyzések:	The main objective of the current study was to obtain preliminary results on genetic polymorhism of A and C variants at the CSN2 locus in indigenous Romanian goat (Banat White, Carpatina) breeds using a rapid and efficient genotyping method, TaqMan assay (Applied Biosystems, USA). Hair follicle samples were taken from 73 Banat White and 82 Carpatina purebred goats from Arad, Caras-Severin and Timis counties. After the optimization of the genotyping assay it was found that the most frequent allele at the CSN2 locus was C in Banat White breed, while CSN2A and CSN2C showed similar frequencies in Carpatina breed (0.51 and 0.49, respectively). All three genotypes were detected in the two studied breeds, however, AA was the least frequent, especially in Banat White. The studied polymorphisms are potential markers for milk production in the studied breeds and the results will be useful in future works aimed at identifying possible associations with milk production traits, in order to test the feasibility of producing hypoallergenic organic goat milk.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk beta-casein gene goats polymorphism Banat White Carpatina hypoallergenic milk
Megjelenés:	Acta Biochimica Polonica. - 63 : 3 (2016), p. 577-580. -
További szerzők:	Ilie, Daniela Elena Sauer, Maria Nagy Krisztina Patras, Irina Gavojdian, Dinu
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9.

001-es BibID:	BIBFORM090372
Első szerző:	Kusza Szilvia (agrármérnök)
Cím:	Preliminary report on β-casein gene Met183QVal183 polymorphism in Romanian indigenous Zackel sheep breeds / Kusza Szilvia, Daniela Elena Ilie, Maria Sauer, Ioan-Walter Sauer, Dinu Gavojdian
Dátum:	2017
ISSN:	0001-527X 1734-154X
Megjegyzések:	Genetic polymorphisms of the milk protein genes are important because of their effects on quantitative traits and technological properties of milk manufacturing. In the present study we identified the polymorphism of the beta-casein gene in two local sheep breeds (Racka n=98 and Turcana n=111) in Romania. The most studied variants at the ovine beta-casein (CSN2) locus are: A and G variants. Genomic DNA was extracted from hair follicles and beta-casein genotypes were determined by the rapid TaqMan (Applied Biosystems, USA) genotyping assay. Homozygote genotypes GG were not detected in any of the studied breeds. In both Racka and Turcana breeds, A variant had a much higher frequency, 0.98% and 0.97%, respectively. In the current study the fast DNA tests for genotyping ovine CSN2 was successfully optimized, however, further samples and correlations of genomic results with milk characteristics and production data are needed for the development of future selection schemes of the Romanian indigenous sheep breeds, with the ultimate purpose to produce low level allergenic sheep milk and derived dairy products.
Tárgyszavak:	Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk gene polymorphism ovine milk Racka Turcana
Megjelenés:	Acta Biochimica Polonica. - 64 : 2 (2017), p. 339-341. -
További szerzők:	Ilie, Daniela Elena Sauer, Maria Sauer, Ioan-Walter Gavojdian, Dinu
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10.

001-es BibID:	BIBFORM079291
035-os BibID:	(cikkazonosító)e4416 (WoS)000426518200008 (Scopus)85042415707 (PMID)29492347 (PMCID)PMC5827040
Első szerző:	Kusza Szilvia (agrármérnök)
Cím:	Kompetitive Allele Specific PCR (KASP TM) genotyping of 48 polymorphisms at different caprine loci in French Alpine and Saanen goat breeds and their association with milk composition / Szilvia Kusza, Ludovic Toma Cziszter, Daniela Elena Ilie, Maria Sauer, Ioan Padeanu, Dinu Gavojdian
Dátum:	2018
ISSN:	2167-8359
Megjegyzések:	Using a novel and fast genotyping method called Kompetitive Allele Specific PCR (KASP TM), we carried out a pilot study on 48 single nucleotide polymorphisms (SNPs) belonging to 40 genes in French Alpine (n = 24) and Saanen (n = 25) goats reared in Romania. Furthermore, the associations of the 13 polymorphic genetic variants with milk production and composition were investigated. Thirty-five SNPs did not show polymorphism in the studied populations. Polymorphic SNPs were detected in the following genes: CAST, CLEC4E, DES, GHRHR, HSP90AA1, IL15RA, IL1RN, IL8, MITF, PPRC1, SOCS3, TNF and TNFSF13. The studied Alpine population was in Hardy-Weinberg disequilibrium at the g.62894878A>G locus (rs671391101) (P < 0.05). The results showed that four SNPs rs671391101 (GHRHR), rs640582069 (IL1RN) rs635583012 (SOCS3) and rs635969404 (IL15RA) out of the 13 polymorphic markers were significantly associated with milk production, protein, fat and lactose content in the Alpine breed. However, no significant effect was recorded in the Saanen population regarding milk yield or milk chemical composition. The current results provide new insights for the development of SNP marker-assisted selection technology in the goat industry and confirm the potential of using SNPs for the GHRHR, IL1RN, SOCS3, and IL15RA genes as candidate genes for selection, highlighting the direct implications of such genes for farm production outputs. The results from this study are relevant for future goat genomic studies and the inclusion of the associated traits into up-to-date selection schemes.
Tárgyszavak:	Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk KASP Saanen Polymorphism Romania French alpine Milk traits Associations
Megjelenés:	PeerJ. - 6 (2018), p. 1-15. -
További szerzők:	Cziszter, Ludovic Toma Ilie, Daniela Elena Sauer, Maria Padeanu, Ioan Gavojdian, Dinu
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11.

001-es BibID:	BIBFORM079245
Első szerző:	Kusza Szilvia (agrármérnök)
Cím:	Study of LGB gene polymorphisms of small ruminants reared in Eastern Europe / Kusza Szilvia, Daniela Elena Ilie, Maria Sauer, Nagy Krisztina, Traian Stefan Atanasiu, Dinu Gavojdian
Dátum:	2018
ISSN:	1212-1819
Megjegyzések:	The objectives of the current research were: (i) to determine the allele and genotype frequencies of the LGB gene in the sheep and goat breeds reared in Eastern Europe; (ii) to implement a comparative study in order to evaluate the milk production potential and efficiency of the main sheep and goat dairy breeds reared in Romania; (iii) to test the genetic basis and feasibility of introducing the molecular-marker assisted selection for this trait into future breeding schemes designed for small ruminants. Totally 731 purebred unrelated sheep (Turcana: 111, Racka: 98, Tsigai: 79, Karakul of Botosani: 60, Transylvanian Merino: 77) and goats (Carpatina: 82, Banat's White: 73, Saanen: 74, French Alpine: 77) were sampled and genotyped for the earlier detected alleles. The frequencies of A (0.44?0.53) and B (0.42?0.51) alleles of LGB gene were generally equal, while the C allele incidence was significantly lower for all sheep breeds studied. A relatively low or missing incidence of the C allele and no BC genotype were detected in the studied sheep breeds. All studied sheep breeds were in Hardy?Weinberg equilibrium. Gene homozygosity was lower than gene heterozygosity, with the effective allele numbers ranging from 2.00 (Transylvanian Merino) to 2.23 (Tsigai). Two alleles (A and B) and two genotypes (AA and AB) were detected in the four goat breeds studied. Allele A was the most frequent allele found in all breeds (0.57?0.68), while AB genotype had the highest frequency. Gene homozygosity was higher than gene heterozygosity, while the effective allele numbers varied between 1.76 (Banat's White) and 1.96 (Carpatina). The frequency of alleles and genotypes was similar to that reported in other Eastern sheep and goat breeds, however polymorphism has not been studied yet among these breeds in such a high number in Romania. An association study between single nucleotide polymorphisms and milk production traits should follow.
Tárgyszavak:	Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban β-LG sheep goats milk production Hungary
Megjelenés:	Czech Journal Of Animal Science. - 63 : 4 (2018), p. 152-159. -
További szerzők:	Ilie, Daniela Elena Sauer, Maria Nagy Krisztina Atanasiu, Traian Stefan Gavojdian, Dinu
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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