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1.

001-es BibID:BIBFORM088048
Első szerző:Gavojdian, Dinu
Cím:Comparative study on fitness traits and reproductive efficiency in Turcana and Tsigai ewes / Gavojdian Dinu, Sauer Maria, Patras Irina, Kusza Szilvia
Dátum:2016
ISSN:2285-5750 2393-2260
Megjegyzések:Aim of the current comparative study was to evaluate health, reproductive rates and fitness indicators in Tsigai and Turcana sheep breeds reared under semi-intensive production system conditions. The project herd is consisted of 203 Tsigai (Szombor ecotype) and 226 Turcana (Sibiu ecotype) breeding ewes. The Tsigai ewes were more affected (p?0.01) by clinical mastitis compared to Turcana ewes, with an incidence of 10.8?0.21% and 3.11?1.16%, respectively. The Tsigai ewes produced significantly higher litters (p?0.001) compared to Turcana breed, 1.45?0.04% compared to 1.17?0.02%. Lambs weaning rates were not influenced by the genotype (p>0.05). This comparative study was the first attempt to provide information on the reproductive efficiency and health traits in Tsigai and Turcana breeds under temperate climate conditions found in Eastern Europe. For the selected specialized Sombor Tsigai ecotype it would be advisable to include fitness traits into the breeding selection schemes in order to improve animal welfare and overall productivity.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Tsigai
Turcana
sheep welfare
reproductive efficiency
Megjelenés:Scientific Papers, Series D. Animal Science. - 59 (2016), p. 193-196. -
További szerzők:Sauer, Maria Patras, Irina Kusza Szilvia (1979-) (agrármérnök)
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2.

001-es BibID:BIBFORM088005
Első szerző:Gavojdian, Dinu
Cím:Effects of using indigenous heritage sheep breeds in organic and low-input production systems on production efficiency and animal welfare in Romania / Gavojdian Dinu, Padeanu Ioan, Sauer Maria, Dragomir Neculai, Ilisiu Elena, Kusza Szilvia, Rahmann Gerold
Dátum:2016
ISSN:0458-6859
Megjegyzések:Romania ranks third in the EU28 in terms of sheep numbers (9.8 millions), having a self-sufficiency for sheep meat of 150 % and therefore exports are important at national level. Objectives of the current research trials were: i) to evaluate the potential of the Turcana breed to produce organic lamb meat under highland pasture-based conditions; ii) to evaluate the overall resistance of the breed and fitness indicators, in comparison with two native breeds, reared under lowinput conditions; iii) to study the genetic base using molecular assisted selection in order to produce hypoallergenic milk and derived milk products. The conventional pasture from the current project had a potential of production of 189 kg/ha for Turcana purebreds, and of 236 kg/ha for F1German Blackheaded x Turcana (GBxTA) crossbreed lambs. While for the organic pasture, it was estimated a production potential of 191 kg of meat per hectar while rearing Turcana purebred lambs, and of 240 kg/ha for the F1GBxTA dualbreeds. In the current preliminary study, the genetic polymorphism of ovine ?-casein gene in the Romanian Zackel breed was highlighted, using TaqMan assay. ?-casein in sheep is strongly correlated to economically important milk quantitative traits, being a first step in introducing genomic selection for the production of organic hypoallergenic sheep milk and derived products.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Romanian Zackel sheep
Turcana sheep
organic lamb production
animal welfare
beta-casein
Rumänisches Zackelschaf
Turcanaschaf
Ökologische Lammfleischproduktion
Tierwohl
beta-Kasein
Megjelenés:Landbauforschung. - 66 : 4 (2016), p. 290-297. -
További szerzők:Padeanu, Ioan Sauer, Maria Dragomir, Neculai Ilisiu, Elena Kusza Szilvia (1979-) (agrármérnök) Rahmann, Gerold
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3.

001-es BibID:BIBFORM079295
035-os BibID:(cikkazonosító)e0197051 (WOS)000431757400057 (Scopus)85046772044
Első szerző:Ilie, Daniela Elena
Cím:Genetic characterization of indigenous goat breeds in Romania and Hungary with a special focus on genetic resistance to mastitis and gastrointestinal parasitism based on 40 SNPs / Daniela Elena Ilie, Kusza Szilvia, Maria Sauer, Dinu Gavojdian
Dátum:2018
ISSN:1932-6203
Megjegyzések:Goat breeding has become an important sector in Eastern Europe, with Romania and Hungary being among the major producer countries. Given the limited number of research done up-to-date concerning genetic studies of indigenous goat breeds reared in Romania and Hungary, the current preliminary study aimed to analyze the variability of genes related to mastitis and gastrointestinal parasitism by using Kompetitive Allele Specific PCR (KASP?). We studied 52 single nucleotide polymorphisms (SNPs) belonging to 19 genes in indigenous breeds from both countries, namely Banat's White (n = 36), Carpatina (n = 35) from Romania and Hungarian Milking (n = 79) and identified 16 polymorphic SNPs among 10 genes (PTX3, IL6, CLEC4E, IL8, IL1RN, IL15RA, TNFSF13, SOCS3, TNF and TLR3) in 150 animals. Furthermore, the diversity of the studied breeds was investigated. The PIC values ranged from 0.042 to 0.691. The mean values of observed and expected heterozygosity were 0.235 and 0.246 respectively. The highest observed heterozygosity was obtained for IL15RA g.10343904C>T in Banat's White (0.464), IL15RA g.10354813C>T in Carpatina (0.577) and SOCS3 g.52626440T>G in Hungarian Milking (0.588). Pairwise FST values between the Romanian breeds and Romanian and Hungarian breeds were small (0.009 and 0.015), indicating the close relationship among the studied goat populations. From all the polymorphic SNPs identified, the Hungarian Milking breed showed the highest proportion of polymorphisms (100%), whereas the Carpatina breed had the lowest percentage (87.5%). The highest value of MAF was obtained for SOCS3 g.52626440T>G (0.46), IL15RA g.10343904C>T (0.47), IL15RA g.10344025C>T (0.45), and IL15RA g.10354813C>T (0.42). The 16 polymorphic SNPs identified in a panel of 150 unrelated individuals belonging to three Romanian and Hungarian indigenous goat breeds could be used in future genomic based breeding schemes as markers for genetic resistance to mastitis and gastrointestinal parasitism in goat breeds found in Eastern and Central Europe.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Plos One. - 13 : 5 (2018), p. 1-14. -
További szerzők:Kusza Szilvia (1979-) (agrármérnök) Sauer, Maria Gavojdian, Dinu
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4.

001-es BibID:BIBFORM087951
Első szerző:Kusza Szilvia (agrármérnök)
Cím:Genetic polymorphism of CSN2 gene in Banat White and Carpatina goats / Kusza Szilvia, Ilie Daniela Elena, Sauer Maria, Nagy Krisztina, Patras Irina, Gavojdian Dinu
Dátum:2016
ISSN:0001-527X 1734-154X
Megjegyzések:The main objective of the current study was to obtain preliminary results on genetic polymorhism of A and C variants at the CSN2 locus in indigenous Romanian goat (Banat White, Carpatina) breeds using a rapid and efficient genotyping method, TaqMan assay (Applied Biosystems, USA). Hair follicle samples were taken from 73 Banat White and 82 Carpatina purebred goats from Arad, Caras-Severin and Timis counties. After the optimization of the genotyping assay it was found that the most frequent allele at the CSN2 locus was C in Banat White breed, while CSN2*A and CSN2*C showed similar frequencies in Carpatina breed (0.51 and 0.49, respectively). All three genotypes were detected in the two studied breeds, however, AA was the least frequent, especially in Banat White. The studied polymorphisms are potential markers for milk production in the studied breeds and the results will be useful in future works aimed at identifying possible associations with milk production traits, in order to test the feasibility of producing hypoallergenic organic goat milk.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
beta-casein gene
goats
polymorphism
Banat White
Carpatina
hypoallergenic milk
Megjelenés:Acta Biochimica Polonica. - 63 : 3 (2016), p. 577-580. -
További szerzők:Ilie, Daniela Elena Sauer, Maria Nagy Krisztina Patras, Irina Gavojdian, Dinu
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5.

001-es BibID:BIBFORM090372
Első szerző:Kusza Szilvia (agrármérnök)
Cím:Preliminary report on β-casein gene Met183QVal183 polymorphism in Romanian indigenous Zackel sheep breeds / Kusza Szilvia, Daniela Elena Ilie, Maria Sauer, Ioan-Walter Sauer, Dinu Gavojdian
Dátum:2017
ISSN:0001-527X 1734-154X
Megjegyzések:Genetic polymorphisms of the milk protein genes are important because of their effects on quantitative traits and technological properties of milk manufacturing. In the present study we identified the polymorphism of the beta-casein gene in two local sheep breeds (Racka n=98 and Turcana n=111) in Romania. The most studied variants at the ovine beta-casein (CSN2) locus are: A and G variants. Genomic DNA was extracted from hair follicles and beta-casein genotypes were determined by the rapid TaqMan (Applied Biosystems, USA) genotyping assay. Homozygote genotypes GG were not detected in any of the studied breeds. In both Racka and Turcana breeds, A variant had a much higher frequency, 0.98% and 0.97%, respectively. In the current study the fast DNA tests for genotyping ovine CSN2 was successfully optimized, however, further samples and correlations of genomic results with milk characteristics and production data are needed for the development of future selection schemes of the Romanian indigenous sheep breeds, with the ultimate purpose to produce low level allergenic sheep milk and derived dairy products.
Tárgyszavak:Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
gene polymorphism
ovine milk
Racka
Turcana
Megjelenés:Acta Biochimica Polonica. - 64 : 2 (2017), p. 339-341. -
További szerzők:Ilie, Daniela Elena Sauer, Maria Sauer, Ioan-Walter Gavojdian, Dinu
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6.

001-es BibID:BIBFORM079291
035-os BibID:(cikkazonosító)e4416 (WoS)000426518200008 (Scopus)85042415707 (PMID)29492347 (PMCID)PMC5827040
Első szerző:Kusza Szilvia (agrármérnök)
Cím:Kompetitive Allele Specific PCR (KASP TM) genotyping of 48 polymorphisms at different caprine loci in French Alpine and Saanen goat breeds and their association with milk composition / Szilvia Kusza, Ludovic Toma Cziszter, Daniela Elena Ilie, Maria Sauer, Ioan Padeanu, Dinu Gavojdian
Dátum:2018
ISSN:2167-8359
Megjegyzések:Using a novel and fast genotyping method called Kompetitive Allele Specific PCR (KASP TM), we carried out a pilot study on 48 single nucleotide polymorphisms (SNPs) belonging to 40 genes in French Alpine (n = 24) and Saanen (n = 25) goats reared in Romania. Furthermore, the associations of the 13 polymorphic genetic variants with milk production and composition were investigated. Thirty-five SNPs did not show polymorphism in the studied populations. Polymorphic SNPs were detected in the following genes: CAST, CLEC4E, DES, GHRHR, HSP90AA1, IL15RA, IL1RN, IL8, MITF, PPRC1, SOCS3, TNF and TNFSF13. The studied Alpine population was in Hardy-Weinberg disequilibrium at the g.62894878A>G locus (rs671391101) (P < 0.05). The results showed that four SNPs rs671391101 (GHRHR), rs640582069 (IL1RN) rs635583012 (SOCS3) and rs635969404 (IL15RA) out of the 13 polymorphic markers were significantly associated with milk production, protein, fat and lactose content in the Alpine breed. However, no significant effect was recorded in the Saanen population regarding milk yield or milk chemical composition. The current results provide new insights for the development of SNP marker-assisted selection technology in the goat industry and confirm the potential of using SNPs for the GHRHR, IL1RN, SOCS3, and IL15RA genes as candidate genes for selection, highlighting the direct implications of such genes for farm production outputs. The results from this study are relevant for future goat genomic studies and the inclusion of the associated traits into up-to-date selection schemes.
Tárgyszavak:Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
KASP
Saanen
Polymorphism
Romania
French alpine
Milk traits
Associations
Megjelenés:PeerJ. - 6 (2018), p. 1-15. -
További szerzők:Cziszter, Ludovic Toma Ilie, Daniela Elena Sauer, Maria Padeanu, Ioan Gavojdian, Dinu
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7.

001-es BibID:BIBFORM079245
Első szerző:Kusza Szilvia (agrármérnök)
Cím:Study of LGB gene polymorphisms of small ruminants reared in Eastern Europe / Kusza Szilvia, Daniela Elena Ilie, Maria Sauer, Nagy Krisztina, Traian Stefan Atanasiu, Dinu Gavojdian
Dátum:2018
ISSN:1212-1819
Megjegyzések:The objectives of the current research were: (i) to determine the allele and genotype frequencies of the LGB gene in the sheep and goat breeds reared in Eastern Europe; (ii) to implement a comparative study in order to evaluate the milk production potential and efficiency of the main sheep and goat dairy breeds reared in Romania; (iii) to test the genetic basis and feasibility of introducing the molecular-marker assisted selection for this trait into future breeding schemes designed for small ruminants. Totally 731 purebred unrelated sheep (Turcana: 111, Racka: 98, Tsigai: 79, Karakul of Botosani: 60, Transylvanian Merino: 77) and goats (Carpatina: 82, Banat's White: 73, Saanen: 74, French Alpine: 77) were sampled and genotyped for the earlier detected alleles. The frequencies of A (0.44?0.53) and B (0.42?0.51) alleles of LGB gene were generally equal, while the C allele incidence was significantly lower for all sheep breeds studied. A relatively low or missing incidence of the C allele and no BC genotype were detected in the studied sheep breeds. All studied sheep breeds were in Hardy?Weinberg equilibrium. Gene homozygosity was lower than gene heterozygosity, with the effective allele numbers ranging from 2.00 (Transylvanian Merino) to 2.23 (Tsigai). Two alleles (A and B) and two genotypes (AA and AB) were detected in the four goat breeds studied. Allele A was the most frequent allele found in all breeds (0.57?0.68), while AB genotype had the highest frequency. Gene homozygosity was higher than gene heterozygosity, while the effective allele numbers varied between 1.76 (Banat's White) and 1.96 (Carpatina). The frequency of alleles and genotypes was similar to that reported in other Eastern sheep and goat breeds, however polymorphism has not been studied yet among these breeds in such a high number in Romania. An association study between single nucleotide polymorphisms and milk production traits should follow.
Tárgyszavak:Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban
β-LG
sheep
goats
milk production
Hungary
Megjelenés:Czech Journal Of Animal Science. - 63 : 4 (2018), p. 152-159. -
További szerzők:Ilie, Daniela Elena Sauer, Maria Nagy Krisztina Atanasiu, Traian Stefan Gavojdian, Dinu
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