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001-es BibID:BIBFORM088005
Első szerző:Gavojdian, Dinu
Cím:Effects of using indigenous heritage sheep breeds in organic and low-input production systems on production efficiency and animal welfare in Romania / Gavojdian Dinu, Padeanu Ioan, Sauer Maria, Dragomir Neculai, Ilisiu Elena, Kusza Szilvia, Rahmann Gerold
Dátum:2016
ISSN:0458-6859
Megjegyzések:Romania ranks third in the EU28 in terms of sheep numbers (9.8 millions), having a self-sufficiency for sheep meat of 150 % and therefore exports are important at national level. Objectives of the current research trials were: i) to evaluate the potential of the Turcana breed to produce organic lamb meat under highland pasture-based conditions; ii) to evaluate the overall resistance of the breed and fitness indicators, in comparison with two native breeds, reared under lowinput conditions; iii) to study the genetic base using molecular assisted selection in order to produce hypoallergenic milk and derived milk products. The conventional pasture from the current project had a potential of production of 189 kg/ha for Turcana purebreds, and of 236 kg/ha for F1German Blackheaded x Turcana (GBxTA) crossbreed lambs. While for the organic pasture, it was estimated a production potential of 191 kg of meat per hectar while rearing Turcana purebred lambs, and of 240 kg/ha for the F1GBxTA dualbreeds. In the current preliminary study, the genetic polymorphism of ovine ?-casein gene in the Romanian Zackel breed was highlighted, using TaqMan assay. ?-casein in sheep is strongly correlated to economically important milk quantitative traits, being a first step in introducing genomic selection for the production of organic hypoallergenic sheep milk and derived products.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Romanian Zackel sheep
Turcana sheep
organic lamb production
animal welfare
beta-casein
Rumänisches Zackelschaf
Turcanaschaf
Ökologische Lammfleischproduktion
Tierwohl
beta-Kasein
Megjelenés:Landbauforschung. - 66 : 4 (2016), p. 290-297. -
További szerzők:Padeanu, Ioan Sauer, Maria Dragomir, Neculai Ilisiu, Elena Kusza Szilvia (1979-) (agrármérnök) Rahmann, Gerold
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2.

001-es BibID:BIBFORM088064
Első szerző:Kusza Szilvia (agrármérnök)
Cím:Mitochondrial DNA variability in Gyimesi Racka and Turcana sheep breeds / Kusza Szilvia, Zakar Erika, Budai Csilla, Cziszter Ludovic-Toma, Padeanu Ioan, Gavojdian Dinu
Dátum:2015
ISSN:0001-527X 1734-154X
Megjegyzések:The current knowledge and documentation on the origins and relationship between Gyimesi Racka reared in Hungary and the Romanian Turcana is rather controversial. Lack of information and scientific reliable proofs for the divergent theories found in the two countries motivated us to implement a trial using molecular methods to assess the genetic distance and diversity in the two breeds. Hair follicles were collected from Gyimesi Racka (2 phenotypes) and from Turcana (6 ecotypes). The 599 bp segment of the D-loop region of the mitochondrial DNA was sequenced. Altogether, 42 haplotypes were identified, while 23 were found in both populations. Populations were highly diverse according to the haplotype and nucleotide diversity indices. AMOVA analysis showed that most of the variation was observed within populations (98%), indicating a weak genetic structure between the two breeds. Animals were grouped into seven groups based on their phenotype; however genetic distances among them were also low. Tajima's D, Fu's Fs, goodness-of-fit statistics, mismatch distribution and network analysis suggested recent demographic expansion. Current comprehensive mtDNA study indicates that there is very low level of genetic differentiation between the Gyimesi Racka and Turcana populations therefore they are de facto one trans-boundary breed.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
farm animal conservation
genetic diversity
mtDNA
trans-boundary breeds
Zackel group
Megjelenés:Acta Biochimica Polonica. - 62 : 2 (2015), p. 273-280. -
További szerzők:Zakar Erika (1986-) Budai Csilla (1987-) (agrár) Cziszter, Ludovic Toma Padeanu, Ioan Gavojdian, Dinu
Pályázati támogatás:TÁMOP-4.2.2/B-10/1-2010-0024
TÁMOP
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3.

001-es BibID:BIBFORM079291
035-os BibID:(cikkazonosító)e4416 (WoS)000426518200008 (Scopus)85042415707 (PMID)29492347 (PMCID)PMC5827040
Első szerző:Kusza Szilvia (agrármérnök)
Cím:Kompetitive Allele Specific PCR (KASP TM) genotyping of 48 polymorphisms at different caprine loci in French Alpine and Saanen goat breeds and their association with milk composition / Szilvia Kusza, Ludovic Toma Cziszter, Daniela Elena Ilie, Maria Sauer, Ioan Padeanu, Dinu Gavojdian
Dátum:2018
ISSN:2167-8359
Megjegyzések:Using a novel and fast genotyping method called Kompetitive Allele Specific PCR (KASP TM), we carried out a pilot study on 48 single nucleotide polymorphisms (SNPs) belonging to 40 genes in French Alpine (n = 24) and Saanen (n = 25) goats reared in Romania. Furthermore, the associations of the 13 polymorphic genetic variants with milk production and composition were investigated. Thirty-five SNPs did not show polymorphism in the studied populations. Polymorphic SNPs were detected in the following genes: CAST, CLEC4E, DES, GHRHR, HSP90AA1, IL15RA, IL1RN, IL8, MITF, PPRC1, SOCS3, TNF and TNFSF13. The studied Alpine population was in Hardy-Weinberg disequilibrium at the g.62894878A>G locus (rs671391101) (P < 0.05). The results showed that four SNPs rs671391101 (GHRHR), rs640582069 (IL1RN) rs635583012 (SOCS3) and rs635969404 (IL15RA) out of the 13 polymorphic markers were significantly associated with milk production, protein, fat and lactose content in the Alpine breed. However, no significant effect was recorded in the Saanen population regarding milk yield or milk chemical composition. The current results provide new insights for the development of SNP marker-assisted selection technology in the goat industry and confirm the potential of using SNPs for the GHRHR, IL1RN, SOCS3, and IL15RA genes as candidate genes for selection, highlighting the direct implications of such genes for farm production outputs. The results from this study are relevant for future goat genomic studies and the inclusion of the associated traits into up-to-date selection schemes.
Tárgyszavak:Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
KASP
Saanen
Polymorphism
Romania
French alpine
Milk traits
Associations
Megjelenés:PeerJ. - 6 (2018), p. 1-15. -
További szerzők:Cziszter, Ludovic Toma Ilie, Daniela Elena Sauer, Maria Padeanu, Ioan Gavojdian, Dinu
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