Találati lista | Tudóstér

001-es BibID:	BIBFORM004699
Első szerző:	Bacsó Zsolt (biofizikus)
Cím:	INF-gamma rearranges membrane topography of MHC-I and ICAM-1 in colon carcinoma cells / Bacso, Z., Bene, L., Damjanovich, L., Damjanovich, S.
Dátum:	2002
Megjegyzések:	Flow-cytometric fluorescence energy transfer (FCET) measurements between fluorescently labeled cell surface MHC-I and ICAM-1 molecules indicated similar receptor patterns in the plasma membrane of interferon-gamma (INF-gamma)-treated colon carcinoma cells as those observed earlier at the surface of lymphoid cells. INF-gamma activation significantly increased the density of MHC-I and ICAM-1 proteins in the membrane. This increase in receptor density was accompanied by decreased proximity level of the homo-associated MHC-I receptors. Hetero-association of MHC-I and ICAM-1 molecules was increased by INF-gamma treatment. INF-gamma changed neither hetero- nor homo-association of transferrin receptors. By staining the sphingomyelin/cholesterol-enriched lipid microdomains with fluorescently labeled cholera toxin B subunit, we found an increase in the amount of lipid-raft associated G(M1)-gangliosides due to INF-gamma treatment. Confocal microscopic results and FCET measurements show that MHC-I and ICAM-1 are components of G(M1)-ganglioside containing lipid-rafts and also support an increase in the size of these lipid-rafts upon INF-gamma treatment.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Carcinoma Cell Membrane chemistry Cholera Toxin Colonic Neoplasms drug effects Energy Transfer Flow Cytometry Fluorescein-5-isothiocyanate Fluorescence G(M1) Ganglioside Histocompatibility Antigens Class I Human Hungary Intercellular Adhesion Molecule-1 Interferon Type II Membrane Microdomains metabolism Microscopy,Confocal pathology pharmacology Protein Binding Receptors,Cell Surface Receptors,Transferrin Support,Non-U.S.Gov't Tumor Cells,Cultured
Megjelenés:	Biochemical and Biophysical Research Communications. - 290 : 2 (2002), p. 635-640. -
További szerzők:	Bene László (1963-) (biofizikus) Damjanovich László (1960-) (általános sebész) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	DOI elektronikus változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM001204
Első szerző:	Bene László (biofizikus)
Cím:	Colorectal carcinoma rearranges cell surface protein topology and density in CD4+ T cells / Bene L., Kanyári Z., Bodnár A., Kappelmayer J., Waldmann T. A., Vámosi G., Damjanovich L.
Dátum:	2007
Megjegyzések:	Previously, we described conserved protein clusters includingMHCI and II glycoproteins, ICAM-1 adhesion molecules, and interleukin- 2 and -15 receptors in lipid rafts of several human cell types. Differential protein interactions can modulate function, thus influence cell fate. Therefore, we analyzed supramolecular clusters of CD4+ T cells from draining lymph nodes and peripheral blood of colorectal carcinoma patients, and compared these to healthy controls. Superclusters of MHC I and II with IL-2/15 receptors were identified by confocal microscopy on all cell types. Flow-cytometricFRETrevealed molecular associations of these proteins with each other and withICAM-1 as well. In draining lymph nodes expression levels of all these proteins were lower, and interactions, particularly between IL-2/15 receptors and MHC molecules weakened or disappeared as compared to the control. Stimuli/local conditions can rearrange cell surface protein patterns on the same cell type in the same patient, having important implications on further function and cell fate.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban CD4+ T cells colorectal carcinoma MHC I MHC II IL-2R IL-15R ICAM-1 receptor clustering FRET Flow cytometry Confocal microscopy Membrane protein association Draining lymph node
Megjelenés:	Biochemical and Biophysical Research Communications. - 361 : 1 (2007), p. 202-207. -
További szerzők:	Kanyári Zsolt (1964-) (orvos) Dóczy-Bodnár Andrea (1970-) (biofizikus) Kappelmayer János (1960-) (laboratóriumi szakorvos) Waldmann, Thomas A. Vámosi György (1967-) (biofizikus) Damjanovich László (1960-) (általános sebész)
Internet cím:	elektronikus változat DOI
Borító:
Saját polcon:

001-es BibID:	BIBFORM005090
Első szerző:	Nagy Péter (biofizikus)
Cím:	ICAM-1 inhibits the homocluster formation of MHC-I in colon carcinoma cells / Nagy, P., Vamosi, G., Damjanovich, S., Damjanovich, L.
Dátum:	2006
ISSN:	006-291X (Print)
Megjegyzések:	ICAM-1 and MHC-I proteins play fundamental roles in antigen presentation, activation of T lymphocytes, and immune responses against tumor cells. Both of them participate in the formation of lipid raft-associated membrane protein clusters. We found significant colocalization between ICAM-1 and MHC-I at the level of large-scale associations. We combined RNA interference and fluorescence resonance energy transfer studies to show that ICAM-1 promotes the partial disassembly of MHC-I homoclusters on LS-174T colon carcinoma cells. Interferon-gamma (IFN-gamma) treatment induced an increase in the expression of MHC-I and ICAM-1 resulting in decreased MHC-I homoassociation. Small interfering RNAs directed against ICAM-1 restored the homoassociation of MHC-I without influencing the expression level of MHC-I by eliminating ICAM-1 molecules interspersed in MHC-I clusters. We conclude that the composition of membrane protein clusters is dynamically altered in response to both physiological and experimentally elicited changes in antigen expression levels.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Antigen Presentation Antigens Biophysics Carcinoma Cell Line, Tumor Cells Colonic Neoplasms Energy Transfer Fluorescence Fluorescence Resonance Energy Transfer genetics Histocompatibility Antigens Histocompatibility Antigens Class I Humans Hungary Intercellular Adhesion Molecule-1 Lymphocytes metabolism Protein Binding Proteins Research RNA,Small Interfering Support T-Lymphocytes
Megjelenés:	Biochemical and Biophysical Research Communications. - 347 : 3 (2006), p. 758-763. -
További szerzők:	Vámosi György (1967-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Damjanovich László (1960-) (általános sebész)
Internet cím:	DOI elektronikus változat
Borító:
Saját polcon: