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001-es BibID:	BIBFORM004858
Első szerző:	Bene László (biofizikus)
Cím:	Detection of receptor trimers on the cell surface by flow cytometric fluorescence energy homotransfer measurements / László Bene, János Szöllősi, Gergely Szentesi, László Damjanovich, Rezső Gaspar, Thomas A. Waldmann, Sándor Damjanovich
Dátum:	2005
ISSN:	0006-3002
Megjegyzések:	Fluorescence energy homotransfer offers a powerful tool for the investigation of the state of oligomerization of cell surface receptors on a cell-by-cell basis by measuring the polarized components of fluorescence intensity of cells labeled with fluorescently stained antibodies. Here we describe homotransfer-based methods for the flow cytometric detection and analysis of hetero- and homo-associations of cell surface receptors. Homotransfer efficiencies for two- and three-body energy transfer interactions are defined and their frequency distribution curves are computed from the fluorescence anisotropy distributions of multiple-labeled cells. The fractions of receptors involved in homo-clustering is calculated based on the dependence of the fluorescence anisotropy on the surface concentration of the fluorescently stained antibodies. A homotransfer analysis of the homo- and hetero-clustering of the MHCI and MHCII glycoproteins, the cytokine receptor IL-2Ralpha, transferrin receptor and the receptor-type tyrosine phosphatase CD45 on JY B and Kit-225-K6 T cells is presented. We investigated how various factors such as the type of dye, rotational mobility of the dye and dye-targeting antibody, as well as the wavelength of the exciting light affect the homotransfer. We show that the homotransfer technique combined with the high statistical resolution of flow cytometry is an effective tool for detecting different oligomeric states of receptors by using fluorophores having restricted rotational mobility on the time scale of fluorescence
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Fluorescence anisotropy Receptor clustering MHCI and MHCII glycoprotein IL-2Rα Transferrin receptor CD45
Megjelenés:	Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1744 : 2 (2005), p. 176-198. -
További szerzők:	Szöllősi János (1953-) (biofizikus) Szentesi Gergely (1976-) (kémia-fizika tanár) Damjanovich László (1960-) (általános sebész) Gáspár Rezső (1944-) (biofizikus) Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM001204
Első szerző:	Bene László (biofizikus)
Cím:	Colorectal carcinoma rearranges cell surface protein topology and density in CD4+ T cells / Bene L., Kanyári Z., Bodnár A., Kappelmayer J., Waldmann T. A., Vámosi G., Damjanovich L.
Dátum:	2007
Megjegyzések:	Previously, we described conserved protein clusters includingMHCI and II glycoproteins, ICAM-1 adhesion molecules, and interleukin- 2 and -15 receptors in lipid rafts of several human cell types. Differential protein interactions can modulate function, thus influence cell fate. Therefore, we analyzed supramolecular clusters of CD4+ T cells from draining lymph nodes and peripheral blood of colorectal carcinoma patients, and compared these to healthy controls. Superclusters of MHC I and II with IL-2/15 receptors were identified by confocal microscopy on all cell types. Flow-cytometricFRETrevealed molecular associations of these proteins with each other and withICAM-1 as well. In draining lymph nodes expression levels of all these proteins were lower, and interactions, particularly between IL-2/15 receptors and MHC molecules weakened or disappeared as compared to the control. Stimuli/local conditions can rearrange cell surface protein patterns on the same cell type in the same patient, having important implications on further function and cell fate.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban CD4+ T cells colorectal carcinoma MHC I MHC II IL-2R IL-15R ICAM-1 receptor clustering FRET Flow cytometry Confocal microscopy Membrane protein association Draining lymph node
Megjelenés:	Biochemical and Biophysical Research Communications. - 361 : 1 (2007), p. 202-207. -
További szerzők:	Kanyári Zsolt (1964-) (orvos) Dóczy-Bodnár Andrea (1970-) (biofizikus) Kappelmayer János (1960-) (laboratóriumi szakorvos) Waldmann, Thomas A. Vámosi György (1967-) (biofizikus) Damjanovich László (1960-) (általános sebész)
Internet cím:	elektronikus változat DOI
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