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1.

001-es BibID:BIBFORM099747
035-os BibID:(WOS)000753681400016 (Scopus)85119967213
Első szerző:Bene László (biofizikus)
Cím:Fluorescence Resonance Energy Transfer Gating by Elliptically Polarized Light on the Cell Surface / Bene László, Gogolák Péter, Ungvári Tamás, Bagdány Miklós, Rubovszky Bálint, Damjanovich László
Dátum:2021
Tárgyszavak:Természettudományok Fizikai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:ACS Photonics. - 8 (2021), p. 3548-3563. -
További szerzők:Gogolák Péter (1968-) (biológus, immunológus) Ungvári Tamás Bagdány Miklós Rubovszky Bálint (1975-) (élettanász) Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:1G3DBLR0TUDF-247
Egyéb
OSTRAT/810/213
Egyéb
TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Sebészet Kutatócsoport
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2.

001-es BibID:BIBFORM099746
035-os BibID:(cikkazonosító)113519
Első szerző:Bene László (biofizikus)
Cím:Excitation polarization angle-resolved single-laser dual-polarization energy transfer on the cell surface / Bene László, Bagdány Miklós, Ungvári Tamás, Rubovszky Bálint, Damjanovich László
Dátum:2021
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Photochemistry & Photobiology, A: Chemistry. - 421 (2021), p. 1-12. -
További szerzők:Bagdány Miklós Ungvári Tamás Rubovszky Bálint (1975-) (élettanász) Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Sebészet Kutatócsoport
1G3DBLR0TUDF-247
Egyéb
OSTRAT/810/213
Egyéb
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3.

001-es BibID:BIBFORM090881
035-os BibID:(cikkazonosító)113144
Első szerző:Bene László (biofizikus)
Cím:Information theoretic FRET calibration on the cell surface / Bene László, Bagdány Miklós, Ungvári Tamás, Damjanovich László
Dátum:2021
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Photochemistry & Photobiology, A: Chemistry. - 409 (2021), p. 1-13. -
További szerzők:Bagdány Miklós Ungvári Tamás Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
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4.

001-es BibID:BIBFORM075134
035-os BibID:(WoS)000444060600014 (Scopus)85052296237
Első szerző:Bene László (biofizikus)
Cím:Dual-Laser Tetra-Polarization FRET (4polFRET) for Site-Selective Control of Homo-FRET in Hetero-FRET Systems on the Cell Surface : the Homo-FRET Gate / Bene L., Bagdány M., Ungvári T., Damjanovich L.
Dátum:2018
ISSN:0003-2700
Megjegyzések:The effects of donor homo-Förster resonance energy transfer (homo-FRET) taking place in hetero-FRET systems is described in the context of hetero-FRET detection via donor and acceptor fluorescence anisotropies in cell surface receptor clusters. Donor homo-FRET can influence both the efficiency of detection as well as the magnitude of the detectable hetero-FRET. A 4-fold polarized FRET detection scheme-tetrapolarization FRET (4polFRET)-is proposed not only for discriminating the effects of homo-FRET from those of hetero-FRET, but also for correlating homo-associations of the donors and acceptors at different donor-acceptor distances, even beyond the critical Förster distance for hetero-FRET ( R0). The method is based on suppressing homo-FRET at the donor side with red-edge excitation. After the anisotropy effects of physical rotation and homo-FRET were separated by site-selective spectroscopy, the magnitude of the effect of homo-FRET on hetero-FRET has been estimated. It has been found significant, offering a new sensitive technique for detecting conformational dynamics via the homo-FRET mediated component of hetero-FRET, the "homo-FRET enhanced hetero-FRET" or "homo-FRET gate". The method is realizable in flow, as well as in image cytometry equipped with polarization detecting facility.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
four-fold fluorescence anisotropy correlations
dual-hetero-FRET
dual-homo-FRET
homo-FRET-sensitized hetero-FRET
donor fluorescence anisotropy
acceptor fluorescence anisotropy
Megjelenés:Analytical Chemistry. - 90 : 17 (2018), p. 10159-10170. -
További szerzők:Bagdány Miklós Ungvári Tamás Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Sebészet Kutatócsoport
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5.

001-es BibID:BIBFORM065560
Első szerző:Bene László (biofizikus)
Cím:Depolarized FRET (depolFRET) on the cell surface : FRET control by photoselection / László Bene, Péter Gogolák, Tamás Ungvári, Miklós Bagdány, István Nagy, László Damjanovich
Dátum:2016
ISSN:0167-4889
Megjegyzések:Sensitivity of FRET in hetero- and homo-FRET systems on the photoselected orientation distribution of donors has been proven by using polarized and depolarized light for excitation. FRET as well as donor and acceptor anisotropies have been simultaneously measured in a dual emission-polarization scheme realized in a conventional flow cytometer by using single laser excitation and applying fluorophore-conjugated mAbs against the MHCI and MHCII cell surface receptors. Depolarization of the originally polarized light have been achieved by using crystal depolarizers based on Cornu's principle, a quarter-wave plate for circular polarization, and a parallel beam splitter acting as a diagonal-polarizer for dual-polarization excitation. Simultaneous analysis of intensity-based FRET efficiency and acceptor depolarization equivocally report that depolarization of light may increase FRET in an amount depending on the acceptor-to-donor concentration ratio. Acceptor depolarization turned to be more sensitive to FRET than donor hyper-polarization and even than intensity-based FRET efficiency. It can be used as a sensitive tool for monitoring changes in the dynamics of the donor-acceptor pairs. The basic observations of FRET enhancement and increased acceptor depolarization obtained for hetero-FRET are paralleled by analog observations of homo-FRET enhancements under depolarized excitation. In terms of the orientation factor for FRET, the FRET enhancements on depolarization in the condition of the macroscopically isotropic orientation distributions such as those of the cell surface bound fluorophores report on the presence of local orientation mismatches of the donor and acceptor preventing the optimal FRET in the polarized case, which may be eliminated by the excitation depolarization. A theory of fluorescence anisotropy for depolarized excitation is also presented.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Polarizationally structured light
Circularly polarized light
Diagonal-polarization
CORNU-depolarizer
Orientation factor for FRET
Homo-FRET
Megjelenés:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. - 1863 : 2 (2016), p. 322-334. -
További szerzők:Gogolák Péter (1968-) (biológus, immunológus) Ungvári Tamás Bagdány Miklós Nagy István (1957-) (villamosmérnök) Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Sebészet Kutatócsoport
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DOI
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6.

001-es BibID:BIBFORM057905
Első szerző:Bene László (biofizikus)
Cím:Dual-laser homo-FRET on the cell surface / László Bene, Tamás Ungvári, Roland Fedor, István Nagy, László Damjanovich
Dátum:2015
ISSN:0167-4889
Megjegyzések:Inhomogeneous broadening and red-edge effects have been detected on a highly mobile system of fluorescently conjugated mAbs targeted to cell surface receptors. By exploiting site-selective spectroscopy and the characteristic loss of homo-FRET on increasing excitation and decreasing emission wavelengths, contributions of physical rotation and homo-FRET to the depolarization of fluorescence anisotropy have been separated. Absolute homo-FRET efficiency has been determined by ratioing two anisotropies: a homo-FRET-sensitive one, which is excited at the absorption main band and detected at the long wavelength region of emission, and a homo-FRET-insensitive one, which is excited at the long wavelength region of absorption and detected at the short wavelength region of emission. Because the anisotropies are simultaneously detected in a unified detection scheme of a dual T-format arrangement, the method is applicable for the real-time tracking of dynamical changes of physical rotations and proximities. The utility of the method is demonstrated in the context of the MHCII molecule and the heavy and light chains of the MHCI molecule, a system of three receptors with well-characterized close mutual proximities. Although the method is presented for a flow cytometer, it can also be realized in a fluorescence microscope capable for dual-laser excitation and dual-anisotropy detection.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Inhomogeneous broadening
Red-edge effect
Directed energy migration FRET (emFRET)
Fluorescence anisotropy
Fluorescence anisotropy lifetime imaging microscopy (rFLIM)
Megjelenés:Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1853 : 5 (2015), p. 1096-1112. -
További szerzők:Ungvári Tamás Fedor Roland (1975-) (sebész) Nagy István (1957-) (villamosmérnök) Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Kardiológia Kutatócsoport
OTKA Bridging Fund support OSTRAT/ 810/213 by the University of Debrecen
OTKA
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7.

001-es BibID:BIBFORM056493
Első szerző:Bene László (biofizikus)
Cím:Single-laser polarization FRET (polFRET) on the cell surface / László Bene, Tamás Ungvári, Roland Fedor, László Damjanovich
Dátum:2014
ISSN:0167-4889
Megjegyzések:A new method for the simultaneous detection of rotational mobility and proximity of cell surface receptors is presented based on cell-by-cell basis measurement of polarized fluorescence intensity components of the donor and acceptor of a FRET system. In addition to the FRET efficiency and the donor and acceptor concentrations, the method makes also possible the determination of the rotational characteristics and the associated fraction of the donors (FRET-fraction). The method is illustrated with flow cytometric and rFLIM measurements on donor-acceptor systems comprising fluorescently labeled whole antibodies and their Fab fragments against epitopes of the MHCI and MHCII cell surface receptors on human lymphoblast cells. Fluorescence anisotropy of donor and acceptor and FRET efficiency were measured for samples of different acceptor-to-donor concentration ratios. Acceptor anisotropy proved to be more sensitive than the donor anisotropy for sensing FRET. After determining the rotational constants of the donor-conjugated antibodies by measurements of FRET in the steady state, and by rFLIM as a reference, the associated fractions of the MHCI and MHCII molecules in their clusters were determined. Besides the flow cytometer and the wide-field rFLIM used in this study, the method can be applied also in other devices capable of dual-anisotropy detection.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Fluorescence anisotropy
Rotational mobility
Proximity
Receptor cluster
FRET-fraction
rFLIM
Megjelenés:Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1843 : 12 (2014), p. 3047-3064. -
További szerzők:Ungvári Tamás Fedor Roland (1975-) (sebész) Damjanovich László (1960-) (általános sebész)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
Szerző által megadott URL
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8.

001-es BibID:BIBFORM049426
Első szerző:Bene László (biofizikus)
Cím:Intensity correlation-based calibration of FRET / László Bene, Tamás Ungvári, Roland Fedor, László Sasi Szabó, László Damjanovich
Dátum:2013
ISSN:0006-3495
Megjegyzések:ABSTRACT Dual-laser flow cytometric resonance energy transfer (FCET) is a statistically efficient and accurate way of determiningproximity relationships for molecules of cells even under living conditions. In the framework of this algorithm, absolutefluorescence resonance energy transfer (FRET) efficiency is determined by the simultaneous measurement of donor-quenchingand sensitized emission. A crucial point is the determination of the scaling factor a responsible for balancing the differentsensitivities of the donor and acceptor signal channels. The determination of a is not simple, requiring preparation of specialsamples that are generally different from a double-labeled FRET sample, or by the use of sophisticated statistical estimation(least-squares) procedures. We present an alternative, free-from-spectral-constants approach for the determination of a andthe absolute FRET efficiency, by an extension of the presented framework of the FCET algorithm with an analysis of the secondmoments (variances and covariances) of the detected intensity distributions. A quadratic equation for a is formulated withthe intensity fluctuations, which is proved sufficiently robust to give accurate a-values on a cell-by-cell basis in a wide systemof conditions using the same double-labeled sample from which the FRET efficiency itself is determined. This seemingly newapproach is illustrated by FRET measurements between epitopes of the MHCI receptor on the cell surface of two cell lines,FT and LS174T. The figures show that whereas the common way of a determination fails at large dye-per-protein labeling ratiosof mAbs, this presented-as-new approach has sufficient ability to give accurate results. Although introduced in a flow cytometer,the new approach can also be straightforwardly used with fluorescence microscopes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
FRET
statistically efficient
Megjelenés:Biophysical Journal. - 105 : 9 (2013), p. 2024-2035. -
További szerzők:Ungvári Tamás Fedor Roland (1975-) (sebész) Sasi Szabó László András (1974-) (sebész) Damjanovich László (1960-) (általános sebész)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
No. ASTF No 201-06
Egyéb
Short-term EMBO fellowship
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9.

001-es BibID:BIBFORM069692
Első szerző:Ungvári Tamás
Cím:Perrin and Förster unified : dual-laser triple-polarization FRET (3polFRET) for interactions at the Förster-distance and beyond / Tamás Ungvári, Péter Gogolák, Miklós Bagdány, László Damjanovich, László Bene
Dátum:2016
ISSN:0167-4889
Megjegyzések:Dual laser flow cytometric energy transfer (FCET)--elaborated by Trón et al. in 1984--is an efficient and rapid way of measuring FRET on large cell populations. FRET efficiency and the donor and acceptor concentrations are determined from one donor and two acceptor signals. In this communication this method is extended towards the domain of receptor dynamics by the detection of polarized components of the three intensities. By enabling a complete description of the proximity and dynamics of FRET-systems, the new measuring scheme allows a more refined description of both the structure and dynamics of cell surface receptor clusters at the nano-scale and beyond. Associated donor fraction, limiting anisotropy and rotational correlation time of the donor, acceptor anisotropy and cell-by-cell estimation of the orientation factor for FRET (K2) are available in the steady state on a single FRET sample in a very rapid and statistically efficient way offered by flow cytometry. For a more sensitive detection of conformational changes the "polarized FRET indices"--quantities composed from FRET efficiency and anisotropies--are proposed. The method is illustrated by measurements on a FRET system with changing FRET-fraction and on a two donor-one acceptor-system, when the existence of receptor trimers are proven by the detection of "hetero-FRET induced homo-FRET relief", i.e. the diminishing of homo-FRET between the two donors in the presence of a donor quencher. The method also offers higher sensitivity for assessing conformational changes at the nano-scale, due to its capability for the simultaneous detection of changes of proximity and relative orientations of the FRET donor and acceptor. Although the method has been introduced in the context of FRET, it is more general: It can be used for monitoring triple-anisotropy correlations also in those cases when FRET actually does not occur, e.g. for interactions occuring beyond the Förster-distance R0. Interpretation of K2 has been extended.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Triple-anisotropy correlations
Donor anisotropy
Acceptor anisotropy
Orientation factor for FRET
Homo-FRET relief
FRET-fraction
Megjelenés:Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1863 : 4 (2016), p. 703-716. -
További szerzők:Gogolák Péter (1968-) (biológus, immunológus) Bagdány Miklós Damjanovich László (1960-) (általános sebész) Bene László (1963-) (biofizikus)
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