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1.

001-es BibID:BIBFORM004699
Első szerző:Bacsó Zsolt (biofizikus)
Cím:INF-gamma rearranges membrane topography of MHC-I and ICAM-1 in colon carcinoma cells / Bacso, Z., Bene, L., Damjanovich, L., Damjanovich, S.
Dátum:2002
Megjegyzések:Flow-cytometric fluorescence energy transfer (FCET) measurements between fluorescently labeled cell surface MHC-I and ICAM-1 molecules indicated similar receptor patterns in the plasma membrane of interferon-gamma (INF-gamma)-treated colon carcinoma cells as those observed earlier at the surface of lymphoid cells. INF-gamma activation significantly increased the density of MHC-I and ICAM-1 proteins in the membrane. This increase in receptor density was accompanied by decreased proximity level of the homo-associated MHC-I receptors. Hetero-association of MHC-I and ICAM-1 molecules was increased by INF-gamma treatment. INF-gamma changed neither hetero- nor homo-association of transferrin receptors. By staining the sphingomyelin/cholesterol-enriched lipid microdomains with fluorescently labeled cholera toxin B subunit, we found an increase in the amount of lipid-raft associated G(M1)-gangliosides due to INF-gamma treatment. Confocal microscopic results and FCET measurements show that MHC-I and ICAM-1 are components of G(M1)-ganglioside containing lipid-rafts and also support an increase in the size of these lipid-rafts upon INF-gamma treatment.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Carcinoma
Cell Membrane
chemistry
Cholera Toxin
Colonic Neoplasms
drug effects
Energy Transfer
Flow Cytometry
Fluorescein-5-isothiocyanate
Fluorescence
G(M1) Ganglioside
Histocompatibility Antigens Class I
Human
Hungary
Intercellular Adhesion Molecule-1
Interferon Type II
Membrane Microdomains
metabolism
Microscopy,Confocal
pathology
pharmacology
Protein Binding
Receptors,Cell Surface
Receptors,Transferrin
Support,Non-U.S.Gov't
Tumor Cells,Cultured
Megjelenés:Biochemical and Biophysical Research Communications. - 290 : 2 (2002), p. 635-640. -
További szerzők:Bene László (1963-) (biofizikus) Damjanovich László (1960-) (általános sebész) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:DOI
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2.

001-es BibID:BIBFORM004858
Első szerző:Bene László (biofizikus)
Cím:Detection of receptor trimers on the cell surface by flow cytometric fluorescence energy homotransfer measurements / László Bene, János Szöllősi, Gergely Szentesi, László Damjanovich, Rezső Gaspar, Thomas A. Waldmann, Sándor Damjanovich
Dátum:2005
ISSN:0006-3002
Megjegyzések:Fluorescence energy homotransfer offers a powerful tool for the investigation of the state of oligomerization of cell surface receptors on a cell-by-cell basis by measuring the polarized components of fluorescence intensity of cells labeled with fluorescently stained antibodies. Here we describe homotransfer-based methods for the flow cytometric detection and analysis of hetero- and homo-associations of cell surface receptors. Homotransfer efficiencies for two- and three-body energy transfer interactions are defined and their frequency distribution curves are computed from the fluorescence anisotropy distributions of multiple-labeled cells. The fractions of receptors involved in homo-clustering is calculated based on the dependence of the fluorescence anisotropy on the surface concentration of the fluorescently stained antibodies. A homotransfer analysis of the homo- and hetero-clustering of the MHCI and MHCII glycoproteins, the cytokine receptor IL-2Ralpha, transferrin receptor and the receptor-type tyrosine phosphatase CD45 on JY B and Kit-225-K6 T cells is presented. We investigated how various factors such as the type of dye, rotational mobility of the dye and dye-targeting antibody, as well as the wavelength of the exciting light affect the homotransfer. We show that the homotransfer technique combined with the high statistical resolution of flow cytometry is an effective tool for detecting different oligomeric states of receptors by using fluorophores having restricted rotational mobility on the time scale of fluorescence
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Fluorescence anisotropy
Receptor clustering
MHCI and MHCII glycoprotein
IL-2Rα
Transferrin receptor
CD45
Megjelenés:Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1744 : 2 (2005), p. 176-198. -
További szerzők:Szöllősi János (1953-) (biofizikus) Szentesi Gergely (1976-) (kémia-fizika tanár) Damjanovich László (1960-) (általános sebész) Gáspár Rezső (1944-) (biofizikus) Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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3.

001-es BibID:BIBFORM004703
035-os BibID:(scopus)0037013728 (wos)000176059200014
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Does mosaicism of the plasma membrane at molecular and higher hierarchical levels in human lymphocytes carry information on the immediate history of cells? / Damjanovich, S., Matyus, L., Damjanovich, L., Bene, L., Jenei, A., Matko, J., Gaspar, R., Szollosi, J.
Dátum:2002
Megjegyzések:A theoretical analysis of experimental data is presented in this mini-review on non-random homo- and hetero-associations of cell surface receptors, which can be recruited in the plasma membrane or at the surface of the rough endoplasmic reticulum during the protein synthesis. In the latter case, the likely genetic origin of these supramolecular formations is analyzed, contrasting this concept to the mobility of the cell surface proteins. A model is offered which, on the one hand, allows the mobility in a restricted way even among microdomain-confined receptor proteins through 'swapping partners'. On the other hand, the lack of mixing molecular components of protein clusters will be analyzed, when homo-and hetero-associations are studied through cell fusion experiments. The most frequently studied cell surface patterns have included lipid raft organized HLA class I and II, ICAM-1, tetraspan molecules, IL2 and IL15 and other receptors, as well. On the contrary coated pit-associated transferrin receptors would not mix with the above lipid raft associated receptor patterns, although transferrin receptor would readily oligomerize into homo-associates. The functional consequences of these superstructures are also analyzed. On the 30th anniversary of the Singer-Nicolson fluid mosaic membrane model one has to pay tribute to the authors, because of their deep insight emphasizing also the mosaicism of the membranes in general and that of the plasma membrane, in particular.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Biophysics
Cell Fusion
Cells
Human
Hungary
Lymphocytes
Proteins
egyetemen (Magyarországon) készült közlemény
Megjelenés:Immunology Letters. - 82 : 1-2 (2002), p. 93-99. -
További szerzők:Mátyus László (1956-) (biofizikus) Damjanovich László (1960-) (általános sebész) Bene László (1963-) (biofizikus) Jenei Attila (1966-) (biofizikus) Matkó János (1952-) (biológus) Gáspár Rezső (1944-) (biofizikus) Szöllősi János (1953-) (biofizikus)
Internet cím:DOI
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4.

001-es BibID:BIBFORM005090
Első szerző:Nagy Péter (biofizikus)
Cím:ICAM-1 inhibits the homocluster formation of MHC-I in colon carcinoma cells / Nagy, P., Vamosi, G., Damjanovich, S., Damjanovich, L.
Dátum:2006
ISSN:006-291X (Print)
Megjegyzések:ICAM-1 and MHC-I proteins play fundamental roles in antigen presentation, activation of T lymphocytes, and immune responses against tumor cells. Both of them participate in the formation of lipid raft-associated membrane protein clusters. We found significant colocalization between ICAM-1 and MHC-I at the level of large-scale associations. We combined RNA interference and fluorescence resonance energy transfer studies to show that ICAM-1 promotes the partial disassembly of MHC-I homoclusters on LS-174T colon carcinoma cells. Interferon-gamma (IFN-gamma) treatment induced an increase in the expression of MHC-I and ICAM-1 resulting in decreased MHC-I homoassociation. Small interfering RNAs directed against ICAM-1 restored the homoassociation of MHC-I without influencing the expression level of MHC-I by eliminating ICAM-1 molecules interspersed in MHC-I clusters. We conclude that the composition of membrane protein clusters is dynamically altered in response to both physiological and experimentally elicited changes in antigen expression levels.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antigen Presentation
Antigens
Biophysics
Carcinoma
Cell Line, Tumor
Cells
Colonic Neoplasms
Energy Transfer
Fluorescence
Fluorescence Resonance Energy Transfer
genetics
Histocompatibility Antigens
Histocompatibility Antigens Class I
Humans
Hungary
Intercellular Adhesion Molecule-1
Lymphocytes
metabolism
Protein Binding
Proteins
Research
RNA,Small Interfering
Support
T-Lymphocytes
Megjelenés:Biochemical and Biophysical Research Communications. - 347 : 3 (2006), p. 758-763. -
További szerzők:Vámosi György (1967-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Damjanovich László (1960-) (általános sebész)
Internet cím:DOI
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5.

001-es BibID:BIBFORM005107
Első szerző:Vámosi György (biofizikus)
Cím:The role of supramolecular protein complexes and membrane potential in transmembrane signaling processes of lymphocytes / Vamosi, G., Bodnar, A., Damjanovich, S., Nagy, P., Varga, Z., Damjanovich, L.
Dátum:2006
ISSN:165-2478 (Print)
Megjegyzések:The formation of protein patterns in lymphocyte plasma membranes is analyzed in the light of past and, also, very recent experiments. The analysis surveys the lateral organization of major histocompatibility complex glycoproteins, intercellular adhesion molecule-1, interleukin-2 and -15 receptors, Kv1.3 K+ ion channels and the T-cell receptor as well as their behavior under different conditions. These molecules form small- and large-scale clusters in the membrane of human lymphocytes. Many of the association motifs occur in other investigated cell types. The conclusions point toward a possible role for ion channel activities, membrane potential changes and alterations of the lateral organization of proteins in transmembrane signaling and cytotoxic interactions. In our outlook new factors that potentially affect membrane protein cluster formation and interactions are discussed. A role for MHC glycoproteins in concentrating membrane proteins and organizing protein patterns is suggested, and the possibility that the membrane potential may modulate protein conformation and, thereby, affect protein-protein interactions is pointed out. A well-defined role for the presence of ion channels in the immune synapse is offered, which could explain the significance of ion channel accumulation in the immune synapse together with the T-cell receptor.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Animals
Biophysics
Cell Membrane
Glycoproteins
Human
Humans
Hungary
immunology
Intercellular Adhesion Molecule-1
Interleukin-2
Ion Channels
Light
Lymphocytes
Major Histocompatibility Complex
Membrane Potentials
Membrane Proteins
metabolism
Multiprotein Complexes
physiology
Protein Conformation
Proteins
Research
Signal Transduction
Support
T-Lymphocytes
Megjelenés:Immunology Letters. - 104 : 1-2 (2006), p. 53-58. -
További szerzők:Dóczy-Bodnár Andrea (1970-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Nagy Péter (1971-) (biofizikus) Varga Zoltán (1969-) (biofizikus, szakfordító) Damjanovich László (1960-) (általános sebész)
Internet cím:DOI
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