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001-es BibID:	BIBFORM001204
Első szerző:	Bene László (biofizikus)
Cím:	Colorectal carcinoma rearranges cell surface protein topology and density in CD4+ T cells / Bene L., Kanyári Z., Bodnár A., Kappelmayer J., Waldmann T. A., Vámosi G., Damjanovich L.
Dátum:	2007
Megjegyzések:	Previously, we described conserved protein clusters includingMHCI and II glycoproteins, ICAM-1 adhesion molecules, and interleukin- 2 and -15 receptors in lipid rafts of several human cell types. Differential protein interactions can modulate function, thus influence cell fate. Therefore, we analyzed supramolecular clusters of CD4+ T cells from draining lymph nodes and peripheral blood of colorectal carcinoma patients, and compared these to healthy controls. Superclusters of MHC I and II with IL-2/15 receptors were identified by confocal microscopy on all cell types. Flow-cytometricFRETrevealed molecular associations of these proteins with each other and withICAM-1 as well. In draining lymph nodes expression levels of all these proteins were lower, and interactions, particularly between IL-2/15 receptors and MHC molecules weakened or disappeared as compared to the control. Stimuli/local conditions can rearrange cell surface protein patterns on the same cell type in the same patient, having important implications on further function and cell fate.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban CD4+ T cells colorectal carcinoma MHC I MHC II IL-2R IL-15R ICAM-1 receptor clustering FRET Flow cytometry Confocal microscopy Membrane protein association Draining lymph node
Megjelenés:	Biochemical and Biophysical Research Communications. - 361 : 1 (2007), p. 202-207. -
További szerzők:	Kanyári Zsolt (1964-) (orvos) Dóczy-Bodnár Andrea (1970-) (biofizikus) Kappelmayer János (1960-) (laboratóriumi szakorvos) Waldmann, Thomas A. Vámosi György (1967-) (biofizikus) Damjanovich László (1960-) (általános sebész)
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001-es BibID:	BIBFORM005090
Első szerző:	Nagy Péter (biofizikus)
Cím:	ICAM-1 inhibits the homocluster formation of MHC-I in colon carcinoma cells / Nagy, P., Vamosi, G., Damjanovich, S., Damjanovich, L.
Dátum:	2006
ISSN:	006-291X (Print)
Megjegyzések:	ICAM-1 and MHC-I proteins play fundamental roles in antigen presentation, activation of T lymphocytes, and immune responses against tumor cells. Both of them participate in the formation of lipid raft-associated membrane protein clusters. We found significant colocalization between ICAM-1 and MHC-I at the level of large-scale associations. We combined RNA interference and fluorescence resonance energy transfer studies to show that ICAM-1 promotes the partial disassembly of MHC-I homoclusters on LS-174T colon carcinoma cells. Interferon-gamma (IFN-gamma) treatment induced an increase in the expression of MHC-I and ICAM-1 resulting in decreased MHC-I homoassociation. Small interfering RNAs directed against ICAM-1 restored the homoassociation of MHC-I without influencing the expression level of MHC-I by eliminating ICAM-1 molecules interspersed in MHC-I clusters. We conclude that the composition of membrane protein clusters is dynamically altered in response to both physiological and experimentally elicited changes in antigen expression levels.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Antigen Presentation Antigens Biophysics Carcinoma Cell Line, Tumor Cells Colonic Neoplasms Energy Transfer Fluorescence Fluorescence Resonance Energy Transfer genetics Histocompatibility Antigens Histocompatibility Antigens Class I Humans Hungary Intercellular Adhesion Molecule-1 Lymphocytes metabolism Protein Binding Proteins Research RNA,Small Interfering Support T-Lymphocytes
Megjelenés:	Biochemical and Biophysical Research Communications. - 347 : 3 (2006), p. 758-763. -
További szerzők:	Vámosi György (1967-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Damjanovich László (1960-) (általános sebész)
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001-es BibID:	BIBFORM075132
035-os BibID:	(WoS)000430916400003 (Scopus)85045892766
Első szerző:	Vámosi György (biofizikus)
Cím:	Turning Noise into Order on the Cell Surface : resonant Activation of Molecular Highlighters / György Vámosi, László Damjanovich, László Bene
Dátum:	2018
ISSN:	1552-4922 1552-4930
Megjegyzések:	MOLECULAR biology has been revolutionized by the discoveryof a new family of fluorophores termed fluorescent proteins(FPs). The value of this kind of fluorophores rests in thepossibility to genetically attach it to virtually all kinds of proteinsand detect it relatively easily via fluorescence spectroscopynot interfering with life processes (1,2). Besides the moreconventional, simple type of FPs with fixed absorption andemission characteristics?that is, with constant absorptionand emission wavelength ranges, and constant absorptioncoefficient and quantum efficiency?there are also those whosephotophysical properties can be modulated by light. This FPsubfamily is called molecular highlighters.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk photoactivation fluorescent protein single molecule counting super-resolution microscopy light induced rotation FRETforce
Megjelenés:	Cytometry Part A. - 93A (2018), p. 397-401. -
További szerzők:	Damjanovich László (1960-) (általános sebész) Bene László (1963-) (biofizikus)
Pályázati támogatás:	TÁMOP-4.2.2.A-11/1/KONV-2012-0045 TÁMOP Sebészet Kutatócsoport GINOP-2.3.2-15-2016-00026 GINOP GINOP-2.3.3-15-2016-00003 GINOP K103965 OTKA TAMOP-4.2.4.A/2-11/1-2012-0001 TÁMOP
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001-es BibID:	BIBFORM005107
Első szerző:	Vámosi György (biofizikus)
Cím:	The role of supramolecular protein complexes and membrane potential in transmembrane signaling processes of lymphocytes / Vamosi, G., Bodnar, A., Damjanovich, S., Nagy, P., Varga, Z., Damjanovich, L.
Dátum:	2006
ISSN:	165-2478 (Print)
Megjegyzések:	The formation of protein patterns in lymphocyte plasma membranes is analyzed in the light of past and, also, very recent experiments. The analysis surveys the lateral organization of major histocompatibility complex glycoproteins, intercellular adhesion molecule-1, interleukin-2 and -15 receptors, Kv1.3 K+ ion channels and the T-cell receptor as well as their behavior under different conditions. These molecules form small- and large-scale clusters in the membrane of human lymphocytes. Many of the association motifs occur in other investigated cell types. The conclusions point toward a possible role for ion channel activities, membrane potential changes and alterations of the lateral organization of proteins in transmembrane signaling and cytotoxic interactions. In our outlook new factors that potentially affect membrane protein cluster formation and interactions are discussed. A role for MHC glycoproteins in concentrating membrane proteins and organizing protein patterns is suggested, and the possibility that the membrane potential may modulate protein conformation and, thereby, affect protein-protein interactions is pointed out. A well-defined role for the presence of ion channels in the immune synapse is offered, which could explain the significance of ion channel accumulation in the immune synapse together with the T-cell receptor.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis Animals Biophysics Cell Membrane Glycoproteins Human Humans Hungary immunology Intercellular Adhesion Molecule-1 Interleukin-2 Ion Channels Light Lymphocytes Major Histocompatibility Complex Membrane Potentials Membrane Proteins metabolism Multiprotein Complexes physiology Protein Conformation Proteins Research Signal Transduction Support T-Lymphocytes
Megjelenés:	Immunology Letters. - 104 : 1-2 (2006), p. 53-58. -
További szerzők:	Dóczy-Bodnár Andrea (1970-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Nagy Péter (1971-) (biofizikus) Varga Zoltán (1969-) (biofizikus, szakfordító) Damjanovich László (1960-) (általános sebész)
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