CCL

Összesen 4 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM001971
Első szerző:Schlammadinger Ágota (belgyógyász, haematológus)
Cím:Bernard-Soulier szindróma : a herediter thrombocytopéniák ritka oka / Schlammadinger Á., Tóth J., Nagy B. jr., Fazakas F., Hársfalvi J., Kappelmayer J., Muszbek L., Radványi G., Boda Z.
Dátum:2007
Tárgyszavak:Orvostudományok Klinikai orvostudományok magyar nyelvű folyóiratközlemény hazai lapban
bernard-soulier-szindróma
herediter macrothrombocytopenia
Megjelenés:Hematológia-Transzfúziológia. - 40 (2007), p. 40-46. -
További szerzők:Tóth Judit (1978-) (laboratóriumi szakorvos) Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Fazakas Ferenc (1969-) (molekuláris biológus) Hársfalvi Jolán (1949-) (klinikai biokémikus) Kappelmayer János (1960-) (laboratóriumi szakorvos) Muszbek László (1942-) (haematológus, kutató orvos) Radványi Gáspár Boda Zoltán (1947-) (belgyógyász, haematologus, klinikai onkológus)
Internet cím:elektronikus változat
Borító:

2.

001-es BibID:BIBFORM010452
Első szerző:Szántó Tímea
Cím:Identification of a VWF peptide antagonist that blocks platelet adhesion under high shear conditions by selectively inhibiting the VWF-collagen interaction / Szanto, T., Vanhoorelbeke, K., Toth, G., Vandenbulcke, A., Toth, J., Noppe, W., Deckmyn, H., Harsfalvi, J.
Dátum:2009
ISSN:1538-7933 (Print)
Megjegyzések:Because the collagen-VWF-GPIb/IX/V axis plays an important role in thrombus formation, it represents a promising target for development of new antithrombotic agents. Objectives: We used phage display to identify potential small peptides that interfere with the VWF-collagen binding and might serve as lead products for the development of possible oral antithrombotic compounds. Methods: A random linear heptamer peptide library was used to select VWF-binding peptides. Results: We identified a phage clone, displaying the YDPWTPS sequence, further referred to as L7-phage, that bound to VWF in a specific and a dose-dependent manner. This L7-phage specifically inhibited the VWF-collagen interaction under both static and flow conditions. Epitope mapping using deletion mutants of VWF revealed that the L7-phage does not bind to the known collagen-binding A3 domain within VWF, but to the more carboxyterminal situated C domain. This inhibition was not due to steric hindrance of the A3 domain-collagen interaction by the L7-phage. Indeed, a tetrabranched multi-antigen peptide (MAP) presenting four copies of the peptide, but not the scrambled MAP, also inhibited VWF-collagen interaction under conditions of high shear stress at a concentration of 148 nmol L<sup>-1</sup>. Conclusions: Based on these results, we conclude that we have identified the first peptide antagonist that binds to the VWF C domain and by this specifically inhibits the VWF binding to collagen, suppressing platelet adhesion and aggregation under high shear conditions. As a consequence, this peptide and its future derivates are potentially interesting antithrombotic agents.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antithrombotic therapy
Collagen
Peptide inhibitor
Phage display
Platelet adhesion
Von Willebrand factor
antithrombocytic agent
collagen
epitope
unclassified drug
von Willebrand factor
amino acid sequence
article
biopanning
carboxy terminal sequence
concentration response
controlled study
deletion mutant
drug mechanism
drug protein binding
drug screening
human
normal human
phage display
priority journal
protein binding
protein domain
protein protein interaction
shear stress
stereospecificity
thrombocyte adhesion
thrombocyte aggregation inhibition
Collagen
Dose-Response Relationship
Drug
Drug Design
Drug Evaluation
Preclinical
Epitope Mapping
Fibrinolytic Agents
Hemorheology
Humans
Oligopeptides
Peptide Library
Platelet Adhesiveness
Platelet Aggregation
Platelet Aggregation Inhibitors
Protein Binding
Stress
Mechanical
von Willebrand Factor
Megjelenés:Journal of Thrombosis and Haemostasis. - 7 : 10 (2009), p. 1680-1687. -
További szerzők:Vanhoorelbeke, Karen Tóth Gábor (Szeged) Vandenbulcke, A. Tóth Judit (1978-) (laboratóriumi szakorvos) Noppe, W. Deckmyn, Hans Hársfalvi Jolán (1949-) (klinikai biokémikus)
Internet cím:elektronikus változat
Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:

3.

001-es BibID:BIBFORM005096
Első szerző:Szarvas Mariann
Cím:Differential platelet deposition onto collagen in cone-and-plate and parallel plate flow chambers / Szarvas, M., Oparaugo, P., Udvardy, M. L., Tóth, J., Szántó, T., Daróczi, L., Vereb, G., Hársfalvi, J.
Dátum:2006
Megjegyzések:To routinely test the formation of thrombi and the effect of drugs modifying it, proper test systems are needed. Their design should rely on the laws of rheology and the physiology of laminar flow. To best model physiological or pathological shear conditions, parallel/linear and rotational type flow chambers are developed. We have compared the initial phase of platelet thrombus formation in a parallel plate flow chamber (PPC) and a cone-and-plate chamber (CPC) under von Willebrand dependent shear conditions. Blood was allowed to flow through human collagen type III surfaces at a shear rate of 1000 s(-1) for 150 s. Thrombus deposition was characterized by surface coverage, average area and height of thrombi. VWF distribution within thrombi was analyzed with confocal laser scanning microscopy. Reduced surface-specific platelet adhesion and aggregation (surface coverage and average thrombus size) were observed in CPC along with a significant increase in single platelet disappearance from the circulating blood. Our data suggest that the higher rate of platelet consumption in this device, as opposed to PPC, is limiting the adhesion to the surface. Consequently, surface-specific processes and aggregation in the flowing blood are both assessed using CPC, while comprehensive evaluation of surface-specific processes is best achieved with PPC. Therefore, the choice of chamber type as a diagnostic tool is purpose-dependent.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
blood
chemistry
Collagen Type III
Computer-Assisted
Confocal
Hemorheology
Human
Humans
Hungary
Image Processing
instrumentation
Linear Models
methods
Microscopy
Non-U.S.Gov't
physiology
Platelet Adhesiveness
Platelet Aggregation
Research
Research Support
Thrombosis
von Willebrand Factor
Megjelenés:Platelets. - 17 : 3 (2006), p. 185-190. -
További szerzők:Oparaugo, Peter Udvardy Miklós László (1977-) (orvos, tudományos segédmunkatárs) Tóth Judit (1978-) (laboratóriumi szakorvos) Szántó Tímea Daróczi Lajos (1965-) (fizikus) Vereb György (1965-) (biofizikus, orvos) Hársfalvi Jolán (1949-) (klinikai biokémikus)
Internet cím:elektronikus változat
DOI
elektronikus változat
Borító:

4.

001-es BibID:BIBFORM009904
Első szerző:Tóth Judit (laboratóriumi szakorvos)
Cím:Increased platelet glycoprotein Ib number, enhanced platelet adhesion and severe cerebral ischaemia in a patient with polycythaemia vera / Toth J., Kappelmayer J., Udvardy M. L., Szanto T., Szarvas M., Rejto L., Soltesz P., Udvardy M., Harsfalvi J.
Dátum:2009
Megjegyzések:The present study describes severe multiplex cerebral ischaemic laesions in a male patient being diagnosed with polycythaemia vera (PV). In contrast to previous publications, unique platelet receptor pattern with normal platelet count was identified. Glycoprotein Ib receptor number on the surface of resting platelets was increased two-fold and almost three-fold in case of activated platelets compared to the controls. More over, in an in vitro study when whole blood was circulated both at venous and arterial shear conditions and shear rate was adjusted according to the blood viscosity, platelet aggregate/thrombus formation was characteristic on surfaces covered with purified von Willebrand factor while in case of controls the surface was covered with single platelets or platelet monolayer. Similar results with pathological findings have not been published in PV until now. Our result undersigns the necessity of antiplatelet therapy of PV patients, even at normal platelet count.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Platelets. - 20 : 4 (2009), p. 282-287. -
További szerzők:Kappelmayer János (1960-) (laboratóriumi szakorvos) Udvardy Miklós László (1977-) (orvos, tudományos segédmunkatárs) Szántó Tímea Szarvas Mariann (1977-) Rejtő László (1963-) (belgyógyász, haematológus) Soltész Pál (1961-) (belgyógyász, kardiológus) Udvardy Miklós (1947-) (belgyógyász, haematológus) Hársfalvi Jolán (1949-) (klinikai biokémikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
elektronikus változat
DOI
Borító:
Rekordok letöltése1