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001-es BibID:BIBFORM047732
035-os BibID:PMID:23907938
Első szerző:Nagy Károly (népegészségügyi felügyelő)
Cím:Susceptibility of lung epithelial cells to alkylating genotoxic insult / Károly Nagy, Róza Ádány, Sándor Szűcs, Balázs Ádám
Dátum:2013
ISSN:0893-6692
Megjegyzések:Alkylation is one of the most common types of DNA damage that can lead to mutations and cancer. Lung is the primary target organ of airborne alkylators such as ethylene oxide (EO). However, the ability of EO to cause lung cancer has not been clearly demonstrated yet. The aim of this study was to investigate the susceptibility of lung cells to alkylating DNA insult by detecting EO-mediated DNA damage with the alkaline comet assay in human lung epithelial cells, peripheral blood lymphocytes, and keratinocytes. The susceptibility of these cell types toward the alkylating insult induced by EO was compared against the oxidative DNA insult induced by hydrogen peroxide (H2O2). Due to the volatility of EO, its active concentrations were monitored by gas chromatography during exposure and were found to decrease significantly in a time-dependent manner. EO induced a statistically significant genotoxic effect at the lowest concentration used (16.4 ?M) in lung epithelial cells and in lymphocytes, while in keratinocytes, a genotoxic effect was not detected until 55.5 ?M EO. However, lung epithelial cells demonstrated increased resistance to oxidative insult. In fact, oxidative DNA damage detectable by endonuclease treatment was minimal in lung cells compared with the other cell types. These results suggest an increased sensitivity of lung epithelial cells toward the alkylating effects of EO, which was not observed for oxidative DNA damage. Our findings point out the importance of DNA alkylation and the possible role of EO on the induction of lung cancer.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
comet assay
alkylation
ethylene oxide
oxidative DNA damage
Megjelenés:Environmental And Molecular Mutagenesis. - 54 : 8 (2013), p. 682-689. -
További szerzők:Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos) Szűcs Sándor (1958-) (biokémikus, vegyész) Ádám Balázs (1973-) (megelőző orvostan és népegészségtan szakorvos)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0031
TÁMOP
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