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001-es BibID:	BIBFORM041280
Első szerző:	Ádám Balázs (megelőző orvostan és népegészségtan szakorvos)
Cím:	Increased genotoxic susceptibility of breast epithelial cells to ethylene oxide / Ádám Balázs, Bárdos Helga, Ádány Róza
Dátum:	2005
ISSN:	1383-5718
Megjegyzések:	This study was carried out with the aim of elucidating the organ-specific effects of ethylene oxide in comparison with the sensitivity of cells from different tissues. An increased incidence of leukemia and lymphoma has been observed in workers exposed to ethylene oxide. However, contradictory findings exist regarding its ability to induce other tumor types, such as breast cancer. We characterized the genotoxicity of ethylene oxide by means of the alkaline version of comet assay in in vitro systems, in order to investigate the hypothesized role of this substance in the development of breast cancer. For this study, we used primary and secondary cultures of lymphoblasts (well-known target cells of the genotoxicity of ethylene oxide), breast epithelial cells (hypothesized target), peripheral blood lymphocytes (cells commonly used in biomonitoring), and of keratinocytes and cervical epithelial cells. DNA damage was measured and expressed as tail DNA, tail length, and tail moment. In the concentration range 0-100 microM, ethylene oxide induced a dose-dependent increase of DNA damage in the investigated cell types without notable cytotoxicity. A statistically significant increase of DNA damage could be observed after treatment with 20 microM ethylene oxide in lymphoblasts (51% increase of tail moment over the background), breast epithelial cells (26% increase) and peripheral lymphocytes (71% increase). In keratinocytes (5% increase) and cervical epithelial cells (5% increase) significant DNA damage could not be detected at this dose, but at higher concentrations (50-100 microM), such an increase was observed. These results are indicative of an increased sensitivity of breast epithelial cells towards genotoxic insults of ethylene oxide. Our observations provide additional data to evaluate the hypothesis that exposure to ethylene oxide may play a role in breast cancer, and the findings may contribute to the development of screening tests for monitoring an early response to genotoxic insults in occupational settings.
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban Ethylene oxide Genotoxicity DNA damage Breast cancer Comet assay
Megjelenés:	Mutation Research-Genetic Toxicology And Environmental Mutagenesis. - 585 : 1-2 (2005), p. 120-126. -
További szerzők:	Bárdos Helga (1969-) (megelőző orvostan és népegészségtan szakorvos) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos)
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001-es BibID:	BIBFORM053222
Első szerző:	Nagy Károly (népegészségügyi felügyelő)
Cím:	Evaluation of the genotoxicity of the pyrethroid insecticide phenothrin / Károly Nagy, Gábor Rácz, Takashi Matsumoto, Róza Ádány, Balázs Ádám
Dátum:	2014
ISSN:	1383-5718
Megjegyzések:	Phenothrin, a synthetic pyrethroid compound, is widely used to control agricultural and household insects, as well as to eliminate human louse infestation. Toxicity studies on the direct DNA-damaging effect of phenothrin are lacking. We therefore investigated whether phenothrin exposure can lead to increased DNA damage in vitro in human peripheral blood lymphocytes and in human hepatocytes. Genotoxicity was evaluated by means of the comet assay modified with formamidopyrimidine DNA-glycosylase post-treatment for the detection of oxidative base-damage in DNA. We also assessed the cytotoxic potential of this compound by use of combined fluorescence viability staining. Our results show that phenothrin induces statistically significant, dose-dependent DNA damage in the absence of marked cytotoxicity at concentrations higher than 20 ?M and 50 ?M in human blood peripheral lymphocytes and hepatocytes, respectively. Oxidative DNA damage could also be detected in the two cell types, although this did not reach statistical significance. These findings provide evidence of the DNA-damaging potential of phenothrin and call for additional studies to reveal the genotoxic properties of this pyrethroid. The observations also point at the importance of using caution when considering the use of phenothrin.
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban Comet assay Pyrethroid Phenothrin Sumithrin DNA damage
Megjelenés:	Mutation Research-Genetic Toxicology And Environmental Mutagenesis. - 770 (2014), p. 1-5. -
További szerzők:	Rácz Gábor (1982-) (orvos, környezetegészségügyi szakember) Matsumoto, Takashi Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos) Ádám Balázs (1973-) (megelőző orvostan és népegészségtan szakorvos)
Pályázati támogatás:	4.2.4. A/2-11-1-2012-0001 Nemzeti Kiválóság Program TÁMOP TÁMOP-4.2.2.A-11/1/KONV-2012-0031 TÁMOP
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