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001-es BibID:	BIBFORM028068
Első szerző:	Szöőr Balázs (biokémikus)
Cím:	Isolation and characterization of the catalytic subunit of protein phosphatase 2A from Neurospora crassa / Balázs Szöőr, Zsigmond Fehér, Éva Bakó, Ferenc Erdődi, Gábor Szabó, Pál Gergely, Viktor Dombrádi
Dátum:	1995
ISSN:	1096-4959
Megjegyzések:	The catalytic subunit of protein phosphatase 2A (PP2Ac) was purified from Neurospora crassa extract by (NH4)2SO4-ethanol precipitation followed by DEAE-Sephacel, heparin-Sepharose, and MonoQ chromatography steps about 900-fold to a specific activity of 1200 U/g with a 2% yield. The apparent M(r) of PP2Ac was estimated to be 35 kDa by gel filtration and 33 kDa by SDS polyacrylamide gel electrophoresis. Half maximal inhibition of PP2Ac was achieved at 0.3 nM okadaic acid, 0.1 nM microcystin-LR, 56 nM cantharidin and 280 nM endothall concentrations. The preparation was completely inhibited by 20 mM NaF, was insensitive to rabbit muscle inhibitor-2, and was specific for the alpha-subunit of rabbit muscle phosphorylase kinase. According to its biochemical properties, N. crassa PP2Ac is very similar to its mammalian counterparts. Antipeptide antibodies raised against the N-terminal and C-terminal ends of human PP2Ac did not cross-react with N. crassa PP2Ac, indicating sequence differences outside the catalytic core of the enzyme.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban protein phosphorylation protein phosphatase 2A Neurospora crassa heparin-Sepharose okadaic acid microcystin-LR cantharidin endothall
Megjelenés:	Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. - 112 : 3 (1995), p. 515-522. -
További szerzők:	Fehér Zsigmond (1949-) (molekuláris genetikus) Szabó Gábor (1927-1996) (biológus, genetikus) Bakó Éva (1958-) (biokémikus) Erdődi Ferenc (1953-) (biokémikus) Gergely Pál (1947-) (biokémikus) Dombrádi Viktor (1953-) (biokémikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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