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1.

001-es BibID:BIBFORM029085
Első szerző:Antal Miklós (orvos, anatómus)
Cím:Direct evidence of an extensive GABAergic innervation of the spinal dorsal horn by fibres descending from the rostral ventromedial medulla / M. Antal, M. Petkó, E. Polgár, C. W. Heizmann, J. Storm-Mathisen
Dátum:1996
Megjegyzések:A long line of studies emphasizes the contribution of serotonergic fibres descending from the rostral ventromedial medulla in the control of spinal nociceptive information processing. A growing body of evidence, however, suggests that the relative contribution of serotonin to the mediation of spinal neuronal activity from the rostral ventromedial medulla may require re-evaluation. It has recently been substantiated that, in addition to the serotonergic fibres, the spinal dorsal horn receives an abundant non-serotonergic projection from the rostral ventromedial medulla. Furthermore, stimulation in the rostral ventromedial medulla could result in a powerful inhibition of nociceptive spinothalamic tract cells without any detectable serotonin release in the dorsal horn. After labelling raphe-spinal axons and axon terminals in the rat by iontophoretic injections of the anterograde axonal tracer Phaseolus vulgaris leucoagglutinin into the central region of the rostral ventromedial medulla (nucleus raphe magnus) and revealing GABA and glycine immunoreactivities of the labelled raphe-spinal terminals and their postsynaptic targets by postembedding immunocytochemical methods, here we demonstrate an extensive GABAergic projection from the rostral ventromedial medulla to the spinal dorsal horn. We show that the majority of the labelled raphe-spinal terminals in laminae I-IIo and IV-V contain GABA and some of the GABA-immunoreactive terminals are also immunoreactive for glycine. We also disclose that GABA-immunoreactive raphe-spinal terminals establish synaptic contacts primarily with GABA- and glycine-negative, presumably excitatory, spinal neurons, including Calbindin-D28k- as well as parvalbumin-immunoreactive cells in both laminae I-IIo and IV-V. The results suggest that volleys in fibres descending from the rostral ventromedial medulla may evoke GABA release from raphe-spinal terminals, and the released GABA, in some cases probably acting together with glycine, might play a crucial, as yet mostly unidentified, role in the inhibition of nociceptive information processing in the dorsal horn of the spinal cord.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Neuroscience. - 73 : 2 (1996), p. 509-518. -
További szerzők:Petkó Mihály (1943-) (orvos, neurobiológus) Polgár Erika Heizmann, C. W. Storm-Mathisen, John
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2.

001-es BibID:BIBFORM029080
Első szerző:Antal Miklós (orvos, anatómus)
Cím:Development of calbindin-D28k immunoreactive neurons in the embryonic chick lumbosacral spinal cord / Miklós Antal, Erika Polgár
Dátum:1993
Megjegyzések:The development of immunoreactivity for the calcium-binding protein calbindin-D28k (CaB) was investigated in the embryonic and hatched chick lumbosacral spinal cord. CaB-immunoreactive neurons were revealed in the dorsal and ventral horns as well as in the intermediate grey matter from early stages of neuronal development. CaB immunoreactivity was first detected in large neurons in the presumptive dorsal horn at embryonic day 5, while small neurons in the lateral dorsal horn were the last to appear, at embryonic day 10. We have identified and traced the morphological maturation of six CaB-immunoreactive cell groups, three in the dorsal horn and three in the ventral horn. In the dorsal horn these groups were (1) large neurons in the lateral dorsal horn (laminae I and IV), (2) small neurons in the lateral dorsal horn (lamina II), and (3) small neurons in the medial dorsal horn (lamina III). All three groups were present throughout the entire length of the lumbosacral spinal cord and showed persistent CaB immunoreactivity. In the ventral horn, CaB-immunoreactive neurons were classified into the following three categories: (1) Neurons dorsal to the lateral motor column (lamina VII). These neurons were present exclusively in the upper lumbosacral segments (LS1-3), and they showed steady CaB immunoreactivity during their maturation. (2) Neurons at the dorsomedial aspect of the lateral motor column (at the border of laminae VII and IX). This population of neurons was characteristic of the lower segments of the lumbosacral cord (LS5-7) and presented transient CaB expression. (3) Neurons within the lateral motor column (lamina IX). These neurons were dispersed throughout the length of the lumbosacral spinal cord. They were three to four times more numerous in the upper than in the lower lumbosacral segments, and their numbers declined throughout LS1-7 as the animal matured. The characteristic features of the development of neurons immunoreactive for CaB are discussed and correlated with previous neuroanatomical and physiological studies concerning sensory and motor functions of the developing chick spinal cord.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) észült közlemény
Megjelenés:European Journal of Neuroscience. - 5 : 7 (1993), p. 782-794. -
További szerzők:Polgár Erika
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3.

001-es BibID:BIBFORM029073
Első szerző:Antal Miklós (orvos, anatómus)
Cím:Distribution of GABA immunoreactivity in the optic tectum of the frog : a light and electron microscopic study / M. Antal
Dátum:1991
ISSN:0306-4522
Megjegyzések:GABA immunoreactivity was studied in the optic tectum of the frog, Rana esculenta, by postembedding immunohistochemical methods at the light and electron microscopic levels. Nearly one-third of the total population of tectal cells appeared to be GABA-immunoreactive. The proportion of stained neurons was highest in layer 9 (61%), and they occurred less frequently in layers 7 (21%) and 6 (27%). Stained perikarya represented a population of small neurons with a diameter of 8-10 microns. Large cell bodies in layer 7 or at the top of layer 6, and cells of origin of the mesencephalic trigeminal tract in layer 2, were devoid of labelling. Axon terminals and dendrites displaying immunoreactivity for GABA were observed in all of the plexiform layers. On the basis of ultrastructural characteristics two types of GABA-positive axon terminals and two variations of GABA-immunoreactive dendrites were distinguished. Synaptic relations of GABA-immunoreactive and GABA-negative axons as well as dendrites were also studied. Besides a wide variety of axodendritic synapses, dendrodendritic synaptic appositions were also revealed. The results suggest that various inhibitory mechanisms are involved in tectal circuits, which have to be incorporated into future neuronal models concerning visual information processing in the optic tectum of the frog.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Neuroscience. - 42 : 3 (1991), p. 879-891. -
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4.

001-es BibID:BIBFORM029094
Első szerző:Antal Miklós (orvos, anatómus)
Cím:Expression of hyperpolarization-activated and cyclic nucleotide-gated cation channel subunit 2 in axon terminals of peptidergic nociceptive primary sensory neurons in the superficial spinal dorsal horn of rats / Antal, M., Papp, I., Bahaerguli, N., Veress, G., Vereb, G.
Dátum:2004
Megjegyzések:Hyperpolarization-activated cyclic nucleotide-gated cation channel proteins (HCN1-4), which are potentially able to modulate membrane excitability, are abundantly expressed by neurons in spinal dorsal root ganglia (DRG). In the present experiment, we investigated whether HCN2 protein is confined exclusively to the perikarya of DRG neurons or is transported from the somata to the central axons of DRG neurons that terminate in the spinal dorsal horn. Using immunohistochemical methods, we have demonstrated that laminae I-IIo of the superficial spinal dorsal horn of the adult rat spinal cord show a strong punctate immunoreactivity for HCN2. Dorsal rhizotomy resulted in a complete loss of immunostaining in the dorsal horn, suggesting that HCN2 is confined to axon terminals of primary afferents. In double labelling immunohistochemical studies, we have also shown that HCN2 widely co-localizes with calcitonin gene-related peptide, but is almost completely segregated from isolectin-B4 binding, indicating that HCN2 is primarily expressed in peptidergic nociceptive primary afferents. The expression of HCN2 in central terminals of peptidergic primary afferents was also verified with electron microscopy. Utilizing the pre-embedding nanogold method, we found that HCN2 is largely confined to axon terminals with dense-core vesicles. Within these terminals, some of the silver grains marking the accurate location of HCN2 molecules were associated with the cell membrane, and others were scattered in the axoplasm. Within the cell membrane, HCN2 was found almost exclusively in extrasynaptic locations. The results suggest that HCN2 may contribute to the modulation of membrane excitability of nociceptive primary afferent terminals in the spinal dorsal horn.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:The European Journal of Neuroscience 19 : 5 (2004), p. 1336-1342. -
További szerzők:Papp Ildikó (1976-) (biológus) Bahaerguli, Niyazi Veress Gábor (1971-) (neurobiológus) Vereb György (1965-) (biofizikus, orvos)
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5.

001-es BibID:BIBFORM028807
Első szerző:Antal Miklós (orvos, anatómus)
Cím:The application of cobalt labelling to electron microscopic investigations of serial sections / Antal M.
Dátum:1984
ISSN:0165-0270
Megjegyzések:The cobalt labelling technique can be applied to ultrathin serial sections and subsequent electron microscopical investigations with the following modifications: a prolonged, up to 12 h, fixation of the tissue in aldehydes; a shortened, 15 min, postfixation in OsO4; embedding in soft resin block by using a higher proportion of plasticizer in the polimerizing mixture; mounting of 5 micrometers thick serial sections between two layers of Agar-Agar coatings; performing the intensification of the Agar section-Agar sandwich with a physical developer containing a low percentage of the reductive agent; reembedding selected thick sections for ultrathin serial sectioning and staining with uranile acetate and lead citrate. The technique unambiguously shows all labelled profiles, and preserves the fine structural details of the surrounding tissues.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Neuroscience Methods. - 12 : 1 (1984), p. 69-77. -
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6.

001-es BibID:BIBFORM028803
Első szerző:Antal Miklós (orvos, anatómus)
Cím:Longitudinal extent of dorsal root fibres in the spinal cord and brain stem of the frog / Antal M., Tornai I., Székely G.
Dátum:1980
ISSN:0306-4522
Megjegyzések:The longitudinal arrangement of dorsal root fibres was investigated with a modified cobalt labelling technique in the spinal cord and brain stem of frogs. The topographical order of dorsal root fibres in the dorsal white column closely resembles the well-known scheme of the mammalian spinal cord. A significant difference between frogs and mammals is the extension of fibres up to the cerebellar plate. The ascending fibres of different origin are organized in concentric rings in the medulla. An oval-shaped area and a triangular area in the dorsal horn, and the motor horn, receive fibre collaterals in the spinal cord. Thoracic dorsal root fibres terminate exclusively in the oval-shaped area. Fibre terminations clearly outline the dorsal column nuclei which begin in the obex region and end at the level of the glossopharyngeal nucleus. The spinal nucleus of the trigeminus is richly supplied by both thin and thick calibre dorsal root fibres in its entire rostrocaudal extension. Two parts of the reticular formation receive dorsal root fibres; the first is in the dorsal gray matter ventral and lateral to the solitary fascicle in the medulla, the second is the lateral reticular zone. In the vestibular region, the medial, lateral and superior vestibular nuclei are innervated by dorsal root fibres. The granular layer of the cerebellum receives a significant contingent of dorsal root fibres. Fibres terminating in the vestibular region and in the cerebellum arise from limb-innervating spinal ganglia. The results indicate a close similarity in the longitudinal arrangement of dorsal root fibres in frogs and in higher vertebrates. The several collaterals that terminate in the hindbrain may modulate the function of the receiving structures. On the basis of present and previous findings the aggregation of primary sensory fibres and the convergence of their terminations are surveyed in the hindbrain.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Neuroscience. - 5 : 7 (1980), p. 1311-1322. -
További szerzők:Tornai István (1954-) (belgyógyász, gasztroenterológus) Székely György (1926-2017) (neurobiológus)
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7.

001-es BibID:BIBFORM004385
Első szerző:Antal Miklós (orvos, anatómus)
Cím:Numbers, densities, and colocalization of AMPA- and NMDA-type glutamate receptors at individual synapses in the superficial spinal dorsal horn of rats / Miklós Antal, Yugo Fukazawa, Mária Eördögh, Dóra Muszil, Elek Molnár, Makoto Itakura, Masami Takahashi, Ryuichi Shigemoto
Dátum:2008
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:The Journal of Neuroscience. - 28 : 39 (2008), p. 9692-9701. -
További szerzők:Fukazawa, Yugo Eördögh Mária Muszil Dóra Molnár Elek Itakura, Makoto Takahashi, Masami Shigemoto, Ryuichi
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8.

001-es BibID:BIBFORM029495
Első szerző:Birinyi András (anatómus, neurobiológus)
Cím:The extent of the dendritic tree and the number of synapses in the frog motoneuron / Birinyi A., Antal M., Wolf E., Székely G.
Dátum:1992
ISSN:0953-816X
Megjegyzések:Frog motoneurons were intracellularly labelled with cobaltic lysine in the brachial and the lumbar segments of the spinal cord, and the material was processed for light microscopy in serial sections. With the aid of the neuron reconstruction system NEUTRACE, the dendritic tree of neurons was reconstructed and the length and surface area of dendrites measured. The surface of somata was determined with the prolate - oblate average ellipsoid calculation. Corrections were made for shrinkage and for optical distortion. The mean surface area of somata was 6710 microm2; lumbar motoneurons were slightly larger than brachial motoneurons. The mean length of the combined dendritic tree of brachial neurons was 29 408 microm and that of lumbar neurons 46 806 microm. The mean surface area was 127 335 microm2 in brachial neurons, and 168 063 microm2 in lumbar neurons. The soma - dendrite surface area ratio was 3 - 5% in most cases. Dendrites with a diameter of </= 1.0 microm constituted approximately 75% of the combined dendritic length in most of the neurons. Unlike in the cat, there was no correlation between the size of stem dendrites and the extent of daughter branches. From the synaptic density estimated in earlier electron microscope investigations of frog motoneuron dendrites (Antal et al., J. Neurocytol., 15, 303 - 310, 1986; 21, 34 - 49, 1992), and from the present data, the number of synapses on the dendritic tree was calculated. The calculations indicated 26 949 synapses on the smallest and 61 519 synapses on the largest neuron if the synaptic density was multiplied by the length of the dendritic tree. If the synaptic density was multiplied by the surface area of the dendritic tree the calculation yielded 23 337 synapses for the smallest and 60 682 synapses for the largest neuron. More than 60% of the combined surface area of dendrites was >600 microm from the soma. This suggests that about two-thirds of the synapses impinged upon distant dendrites >600 microm from the soma. The efficacy of synapses at these large distances is investigated on model neurons in the accompanying paper
Tárgyszavak:Orvostudományok Természettudományok Biológiai tudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:European Journal Of Neuroscience. - 4 : 11 (1992), p. 1003-1012. -
További szerzők:Antal Miklós (1951-) (orvos, anatómus) Wolf Ervin (1961-) (fizikus, neurobiológus) Székely György (1926-2017) (neurobiológus)
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9.

001-es BibID:BIBFORM029090
Első szerző:Dityatev, A. E.
Cím:A correlative physiological and morphological analysis of monosynaptically connected propriospinal axon - motoneuron pairs in the lumbar spinal cord of frogs / Dityatev A. E., Birinyi A., Puskár Z., Antal M., Clamann P.
Dátum:2001
ISSN:0306-4522
Megjegyzések:Intracellular stimulation of single propriospinal axons evoked excitatory postsynaptic potentials (EPSPs) in lumbar motoneurons. Mean EPSP amplitudes differed by two orders of magnitude when measured in different connections. After analyzing the distribution of mean amplitudes of 47 single-fiber EPSPs, two populations of responses could be defined: (1) those with mean amplitudes between 0.1 and 1.2 mV (mean+/-S.D.: 0.48+/-0.30 mV, 34 pairs), which is in the range of values typical for single-fiber EPSPs evoked by stimulation of supraspinal fibers and primary muscle afferents, (2) those with mean amplitudes between 1.6 and 8 mV (4.2+/-2.0 mV, 13 pairs). Both populations of responses had similarly short latencies and rise times and responded similarly to paired-pulse stimulation, consistent with monosynaptic transmission. However, the high-efficacy connections had significantly smaller coefficients of variation of EPSPs, as well as increased quantal content and quantal size. Tetanic stimulation gradually depressed the amplitude of large EPSPs by 81-86%, but did not affect small EPSPs. Recovery of large EPSPs was exponential with a time constant of 3-5.6 min. During post-tetanic depression the amplitude ratio between the test and conditioned EPSPs evoked by paired-pulse stimulation was not changed but the coefficient of variation was increased, suggesting that the depression was due to depletion of synaptic vesicles available for release.Intracellular labeling of seven electrophysiologically studied propriospinal axon-motoneuron pairs revealed that the number of axon varicosities establishing close appositions with dendrites of the labeled motoneuron was higher for connections where large-amplitude EPSPs were recorded. These varicosities were more often located on proximal dendrites of motoneurons than those of low-efficacy connections. In addition, the number of boutons in highly effective connections was several times lower than the maximal number of available quanta estimated from physiological data, implying that the large EPSPs may be generated by multivesicular release from presynaptic boutons. We conclude that the efficacy and related mode of use-dependent modulation of propriospinal connections is determined by a number of factors, including the number and position of synaptic contacts and the number of active zones or vesicles available for release.
Tárgyszavak:Orvostudományok Elméleti orvostudományok magyar nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Neuroscience. - 106 : 2 (2001), p. 405-417. -
További szerzők:Birinyi András (1960-) (anatómus, neurobiológus) Puskár Zita Antal Miklós (1951-) (orvos, anatómus) Clamann, P.
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10.

001-es BibID:BIBFORM067497
Első szerző:Dócs Klaudia (orvos)
Cím:The Ratio of 2-AG to Its Isomer 1-AG as an Intrinsic Fine Tuning Mechanism of CB1 Receptor Activation / Klaudia Dócs, Zoltán Mészár, Sándor Gonda, Attila Kiss-Szikszai, Krisztina Holló, Miklós Antal, Zoltán Hegyi
Dátum:2017
ISSN:1662-5102
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Frontiers in Cellular Neuroscience. - 11 (2017), p. 1-13. -
További szerzők:Mészár Zoltán Mihály (1977-) (agrármérnök) Gonda Sándor (1984-) (gyógyszerész) Kiss-Szikszai Attila (1975-) (vegyész) Holló Krisztina (1967-) (vegyész) Antal Miklós (1951-) (orvos, anatómus) Hegyi Zoltán (1983-) (molekuláris biológus)
Pályázati támogatás:MTA-TKI 242
MTA
KTIA_NAP_13-1-2013-001
Egyéb
PD 108467
OTKA
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11.

001-es BibID:BIBFORM029072
Első szerző:Gulyás Adrienn (kutatóorvos)
Cím:Septal GABAergic neurons innervate inhibitory interneurons in the hippocampus of the macaque monkey / A. I. Gulyás, L. Seress, K. Tóth, L. Acsády, M. Antal, T. F. Freund
Dátum:1991
ISSN:0306-4522
Megjegyzések:The septohippocampal projection was visualized in three Macaca mulatta monkeys by anterograde transport of Phaseolus vulgaris leucoagglutinin. Following injections of the lectin into the medial septal nucleus, P. vulgaris leucoagglutinin-labelled fibres were found in the hippocampal complex, mainly in stratum oriens of the CA1 subfield, throughout the CA3 subfield, and in the hilus and stratum moleculare of the dentate gyrus. The majority of labelled axons were varicose, and formed multiple contacts with cell bodies and dendrites of calbindin D28k- and parvalbumin-immunoreactive non-pyramidal cells. GABA immunoreactivity of P. vulgaris leucoagglutinin-labelled axons and their postsynaptic targets was investigated by sectioning varicose axon segments for correlated light and electron microscopy, and processing alternate ultrathin sections for postembedding immunogold staining for GABA. All P. vulgaris leucoagglutinin-labelled boutons examined were GABA-immunoreactive and the majority of them formed symmetrical synapses with GABA-immunoreactive cell bodies and dendrites. The results demonstrate that a GABAergic septohippocampal pathway exists in the monkey, and, similar to the rat, terminates on different types of GABAergic neurons, including the parvalbumin- and calbindin D28k-containing non-pyramidal cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Neuroscience. - 41 : 2-3 (1991), p. 381-390. -
További szerzők:Seress László (1950-) (orvos) Tóth K. Acsády L. Freund Tamás F. Antal Miklós (1951-) (orvos, anatómus)
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12.

001-es BibID:BIBFORM008789
Első szerző:Hegyi Zoltán (molekuláris biológus)
Cím:Neuronal and glial localization of the cannabinoid-1 receptor in the superficial spinal dorsal horn of the rodent spinal cord / Zoltán Hegyi, Gréta Kis, Krisztina Holló, Catherine Ledent, Miklós Antal
Dátum:2009
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:European Journal of Neuroscience. - 30 : 2 (2009), p. 251-262. -
További szerzők:Kis Gréta (1979-) (környezetkutató) Holló Krisztina (1967-) (vegyész) Ledent, Catherine Antal Miklós (1951-) (orvos, anatómus)
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