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1.

001-es BibID:BIBFORM029085
Első szerző:Antal Miklós (orvos, anatómus)
Cím:Direct evidence of an extensive GABAergic innervation of the spinal dorsal horn by fibres descending from the rostral ventromedial medulla / M. Antal, M. Petkó, E. Polgár, C. W. Heizmann, J. Storm-Mathisen
Dátum:1996
Megjegyzések:A long line of studies emphasizes the contribution of serotonergic fibres descending from the rostral ventromedial medulla in the control of spinal nociceptive information processing. A growing body of evidence, however, suggests that the relative contribution of serotonin to the mediation of spinal neuronal activity from the rostral ventromedial medulla may require re-evaluation. It has recently been substantiated that, in addition to the serotonergic fibres, the spinal dorsal horn receives an abundant non-serotonergic projection from the rostral ventromedial medulla. Furthermore, stimulation in the rostral ventromedial medulla could result in a powerful inhibition of nociceptive spinothalamic tract cells without any detectable serotonin release in the dorsal horn. After labelling raphe-spinal axons and axon terminals in the rat by iontophoretic injections of the anterograde axonal tracer Phaseolus vulgaris leucoagglutinin into the central region of the rostral ventromedial medulla (nucleus raphe magnus) and revealing GABA and glycine immunoreactivities of the labelled raphe-spinal terminals and their postsynaptic targets by postembedding immunocytochemical methods, here we demonstrate an extensive GABAergic projection from the rostral ventromedial medulla to the spinal dorsal horn. We show that the majority of the labelled raphe-spinal terminals in laminae I-IIo and IV-V contain GABA and some of the GABA-immunoreactive terminals are also immunoreactive for glycine. We also disclose that GABA-immunoreactive raphe-spinal terminals establish synaptic contacts primarily with GABA- and glycine-negative, presumably excitatory, spinal neurons, including Calbindin-D28k- as well as parvalbumin-immunoreactive cells in both laminae I-IIo and IV-V. The results suggest that volleys in fibres descending from the rostral ventromedial medulla may evoke GABA release from raphe-spinal terminals, and the released GABA, in some cases probably acting together with glycine, might play a crucial, as yet mostly unidentified, role in the inhibition of nociceptive information processing in the dorsal horn of the spinal cord.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Neuroscience. - 73 : 2 (1996), p. 509-518. -
További szerzők:Petkó Mihály (1943-) (orvos, neurobiológus) Polgár Erika Heizmann, C. W. Storm-Mathisen, John
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM029080
Első szerző:Antal Miklós (orvos, anatómus)
Cím:Development of calbindin-D28k immunoreactive neurons in the embryonic chick lumbosacral spinal cord / Miklós Antal, Erika Polgár
Dátum:1993
Megjegyzések:The development of immunoreactivity for the calcium-binding protein calbindin-D28k (CaB) was investigated in the embryonic and hatched chick lumbosacral spinal cord. CaB-immunoreactive neurons were revealed in the dorsal and ventral horns as well as in the intermediate grey matter from early stages of neuronal development. CaB immunoreactivity was first detected in large neurons in the presumptive dorsal horn at embryonic day 5, while small neurons in the lateral dorsal horn were the last to appear, at embryonic day 10. We have identified and traced the morphological maturation of six CaB-immunoreactive cell groups, three in the dorsal horn and three in the ventral horn. In the dorsal horn these groups were (1) large neurons in the lateral dorsal horn (laminae I and IV), (2) small neurons in the lateral dorsal horn (lamina II), and (3) small neurons in the medial dorsal horn (lamina III). All three groups were present throughout the entire length of the lumbosacral spinal cord and showed persistent CaB immunoreactivity. In the ventral horn, CaB-immunoreactive neurons were classified into the following three categories: (1) Neurons dorsal to the lateral motor column (lamina VII). These neurons were present exclusively in the upper lumbosacral segments (LS1-3), and they showed steady CaB immunoreactivity during their maturation. (2) Neurons at the dorsomedial aspect of the lateral motor column (at the border of laminae VII and IX). This population of neurons was characteristic of the lower segments of the lumbosacral cord (LS5-7) and presented transient CaB expression. (3) Neurons within the lateral motor column (lamina IX). These neurons were dispersed throughout the length of the lumbosacral spinal cord. They were three to four times more numerous in the upper than in the lower lumbosacral segments, and their numbers declined throughout LS1-7 as the animal matured. The characteristic features of the development of neurons immunoreactive for CaB are discussed and correlated with previous neuroanatomical and physiological studies concerning sensory and motor functions of the developing chick spinal cord.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) észült közlemény
Megjelenés:European Journal of Neuroscience. - 5 : 7 (1993), p. 782-794. -
További szerzők:Polgár Erika
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM029075
Első szerző:Antal Miklós (orvos, anatómus)
Cím:Different populations of parvalbumin- and calbindin-D28k-immunoreactive neurons contain GABA and accumulate 3H-D-aspartate in the dorsal horn of the rat spinal cord / M. Antal, E. Polgár, J. Chalmers, J. B. Minson, I. Llewellyn-Smith, C. W. Heizmann, P. Somogyi
Dátum:1991
Megjegyzések:The colocalization of parvalbumin (PV), calbindin-D28k (CaBP), GABA immunoreactivities, and the ability to accumulate 3H-D-aspartate selectively were investigated in neurons of laminae I-IV of the dorsal horn of the rat spinal cord. Following injection of 3H-D-aspartate into the basal dorsal horn (laminae IV-VI), perikarya selectively accumulating 3H-D-aspartate were detected in araldite embedded semithin sections by autoradiography, and consecutive semithin sections were treated to reveal PV, CaBP and GABA by postembedding immunocytochemistry. Perikarya accumulating 3H-D-aspartate were found exclusively in laminae I-III, and no labelled somata were found in deeper layers or in the intermediolateral column although the labelled amino acid clearly spread to these regions. More than half of the labelled cells were localized in lamina II. In this layer, 16.4% of 3H-D-aspartate-labelled perikarya were also stained for CaBP. In contrast to CaBP, PV or GABA was never detected in neurons accumulating 3H-D-aspartate. A high proportion of PV-immunoreactive perikarya were also stained for GABA in laminae II and III (70.0% and 61.2% respectively). However, the majority of CaBP-immunoreactive perikarya were GABA-negative. GABA-immunoreactivity was found in less than 2% of the total population of cells stained for CaBP in laminae I-IV. A significant proportion of the GABA-negative but PV-immunoreactive neurons also showed CaBP-immunoreactivity in laminae II and IV. These results show that out of the two calcium-binding proteins, CaBP is a characteristic protein of a small subpopulation of neurons using excitatory amino acids and PV is a characteristic protein of a subpopulation of neurons utilizing GABA as a transmitter. However, both proteins are present in additional subgroups of neurons, and neuronal populations using inhibitory or excitatory amino acid transmitters are heterogeneous with regard to their content of calcium-binding proteins in the dorsal horn of the rat spinal cord.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:The Journal of comparative neurology. - 314 : 1 (1991), p. 114-124. -
További szerzők:Polgár Erika Chalmers, J. Minson, J. B. Llewellyn-Smith, I. Heizmann, C. W. Somogyi Péter
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM029060
Első szerző:Antal Miklós (orvos, anatómus)
Cím:Calcium-binding proteins, parvalbumin- and calbindin-D 28k-immunoreactive neurons in the rat spinal cord and dorsal root ganglia : a light and electron microscopic study / Miklós Antal, Tamás F. Freund, Erika Polgár
Dátum:1990
ISSN:0021-9967
Megjegyzések:The distribution of two calcium-binding proteins, parvalbumin (PV) and calbindin-D 28K (CaBP), was studied by the peroxidase-anti-peroxidase immunohistochemical method at the light and electron microscopic level in the rat spinal cord and dorsal root ganglia. The possible coexistence of these two proteins was also investigated. PV-positive neurons were revealed in all layers of the spinal cord, except lamina I, which was devoid of labelling. Most of the PV-positive cells were found in the inner layer of lamina II, lamina III, internal basilar nucleus, central gray region, and at the dorsomedial and ventromedial aspects of the lateral motor column in the ventral horn. Neuronal processes intensely stained for PV sharply delineated inner lamina II. With the electron microscope most of them appeared to be dendrites, but vesicle containing profiles were also found in a smaller number. CaBP-positive neurons appeared to be dispersed all over the spinal gray matter. The great majority of them were found in laminae I, II, IV; the central gray region; the intermediolateral nucleus; and in the ventral horn just medial to the lateral motor column. Laminae I and II were densely packed with CaBP-positive punctate profiles that proved to be dendrites and axons in the electron microscope. A portion of labelled neurons in lamina IV and on the ventromedial aspect of the lateral motor column in the ventral horn disclosed both PV- and CaBP-immunoreactivity. All of the funiculi of the spinal white matter contained a large number of fibres immunopositive for both PV and CaBP. The highest density of CaBP-positive fibres was found in the dorsolateral funiculus, which was also densely packed with PV-positive fibres. PV-positive fibres were even more numerous in the dorsal part of the dorsal funiculus. The territory of the gracile funiculus in the brachial cord and that of the pyramidal tract in its whole extent were devoid of labelled fibres. In the thoracic cord, the dorsal nucleus of Clarke received a large number of PV-positive fibres. Dorsal root ganglia displayed both PV- and CaBP-immunopositivity. The cell diameter distribution histogram of PV-positive neurons disclosed two peaks--one at 35 microns and the other at 50 microns. CaBP-positive cells in the dorsal root ganglia corresponded to subgroups of small and large neurons with mean diameters of 25 microns and 45 microns, respectively.(ABSTRACT TRUNCATED AT 400 WORDS)
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Comparative Neurology. - 295 : 3 (1990), p. 467-484. -
További szerzők:Freund Tamás F. Polgár Erika
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM029081
Első szerző:Nagy István (orvos)
Cím:Combination of cobalt labelling with immunocytochemical reactions for electron microscopic investigations on frog spinal cord / István Nagy, Attila Sík, Erika Polgár, Mihály Petkó, Miklós Antal
Dátum:1994
Megjegyzések:Cobalt staining of primary afferents in frog spinal cord was combined with peroxidase-antiperoxidase pre-embedding or immunogold post-embedding immunocytochemical labelling. Our results have shown that cobalt labelling can easily be distinguished from both of the immunoreaction end products. The protocol of cobalt labelling did not affect the immunoreactivity of structures. The morphology and synaptology of cobalt labelled and immunostained profiles in our sections were very similar to those reported in previous studies using different double labelling techniques. These results indicate that this new combined method could be used as an alternative double labelling technique in electron microscopic studies on nervous tissues.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Microscopy research and technique. - 28 : 1 (1994), p. 60-66. -
További szerzők:Sík Attila Polgár Erika Petkó Mihály (1943-) (orvos, neurobiológus) Antal Miklós (1951-) (orvos, anatómus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:BIBFORM029083
Első szerző:Polgár Erika
Cím:The colocalization of parvalbumin and calbindin-D28k with GABA in the subnucleus caudalis of the rat spinal trigeminal nucleus / E. Polgár, M. Antal
Dátum:1995
ISSN:0014-4819
Megjegyzések:The colocalization of two calcium-binding proteins, parvalbumin (PV) and calbindin-D28k (CaB), which have been reported to be markers of specific subpopulations of neurons in the central nervous system, with the inhibitory amino acid neurotransmitter gamma-amino-butyric acid (GABA) was investigated in neurons of laminae I-IV of the subnucleus caudalis of the rat spinal trigeminal nucleus by using post-embedding immunocytochemical methods. Cells immunoreactive for PV, CaB, and GABA were found in all four laminae of the subnucleus caudalis. A substantial proportion of PV-immunoreactive perikarya were also stained for GABA in laminae II and III (44.8% and 39.8%, respectively). However, the majority of PV-containing neurons in laminae I and IV (100% and 86%, respectively), as well as CaB-immunoreactive cells in all four laminae (98.4%), were GABA-negative. These results show that, in contrast to higher brain centers, PV-, CaB-, and GABA-immunoreactive perikarya represent significantly different populations of neurons in the subnucleus caudalis of the rat. In the light of the present findings, the differences in the neurochemical properties of the subnucleus caudalis of the spinal trigeminal nucleus and the spinal dorsal horn are also discussed.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Experimental Brain Research. - 103 : 3 (1995), p. 402-408. -
További szerzők:Antal Miklós (1951-) (orvos, anatómus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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