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1.

001-es BibID:BIBFORM029082
Első szerző:Antal Miklós (orvos, anatómus)
Cím:Developmental changes in the distribution of gamma-aminobutyric acid-immunoreactive neurons in the embryonic chick lumbosacral spinal cord / Miklós Antal, Ágnes Cs. Berki, László Horváth, Michael O'Donovan
Dátum:1994
ISSN:0021-9967
Megjegyzések:The development of gamma-aminobutyric acid (GABA)-immunoreactive neurons was investigated in the embryonic and posthatch chick lumbosacral spinal cord by using pre- and postembedding immunostaining with an anti-GABA antiserum. The first GABA-immunoreactive cells were detected in the ventral one-half of the spinal cord dorsal to the lateral motor column at E4. GABAergic neurons in this location sharply increased in number and, with the exception of the lateral motor column, appeared throughout the entire extent of the ventral one-half of the spinal gray matter by E6. Thereafter, GABA-immunoreactive neurons extended from ventral to dorsal regions. Stained perikarya first appeared at E8 and then progressively accumulated in the dorsal horn, while immunoreactive neurons gradually declined in the ventral horn. The general pattern of GABA immunoreactivity characteristic of mature animals had been achieved by E12 and was only slightly altered afterwards. In the dorsal horn, most of the stained neurons were observed in laminae I-III, both at the upper (LS 1-3) and at the lower (LS 5-7) segments of the lumbosacral spinal cord. In the ventral horn, the upper and lower lumbosacral segments showed marked differences in the distribution of stained perikarya. GABAergic neurons were scattered in a relatively large region dorsomedial to the lateral motor column at the level of the upper lumbosacral segments, whereas they were confined to the dorsalmost region of lamina VII at the lower segments. The early expression of GABA immunoreactivity may indicate a trophic and synaptogenetic role for GABA in early phases of spinal cord development.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Comparative Neurology. - 343 : 2 (1994), p. 228-236. -
További szerzők:Berki Ágnes Cs. Horváth László O'Donovan, Michael
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM029084
Első szerző:Berki Ágnes Cs.
Cím:Developmental expression of glycine immunoreactivity and its colocalization with GABA in the embryonic chick lumbosacral spinal cord / Ágnes Cs. Berki, Michael J. O'Donovan, Miklós Antal
Dátum:1995
ISSN:0021-9967
Megjegyzések:The development of immunoreactivity for the putative inhibitory amino acid neurotransmitter glycine was investigated in the embryonic and posthatched chick lumbosacral spinal cord by using postembedding immunocytochemical methods. Glycine immunoreactive perikarya were first observed at embryonic day 8 (E8) both in the dorsal and ventral gray matters. The number of immunostained neurons sharply increased by E10 and was gradually augmented further at later developmental stages. The general pattern of glycine immunoreactivity characteristic of mature animals had been achieved by E12 and was only slightly altered afterward. Most of the immunostained neurons were located in the presumptive deep dorsal horn (laminae IV-VI) and lamina VII, although glycine-immunoreactive neurons were scattered throughout the entire extent of the spinal gray matter. By using some of our previously obtained and published data concerning the development of gamma-aminobutyric acid (GABA)-ergic neurons in the embryonic chick lumbosacral spinal cord, we have compared the numbers, sizes, and distribution of glycine- and GABA-immunoreactive spinal neurons at various developmental stages and found the following marked differences in the developmental characteristics of these two populations of putative inhibitory interneurons. (i) GABA immunoreactivity was expressed very early (E4), whereas immunoreactivity for glycine appeared relatively late (E8) in embryonic development. (ii) In the ventral horn, GABA immunoreactivity declined, whereas immunoreactivity for glycine gradually increased from E8 onward in such a manner that the sum of glycinergic and GABAergic perikarya remained constant during the second half of embryonic development. (iii) Glycinergic and GABAergic neurons showed different distribution patterns in the spinal gray matter throughout the entire course of embryogenesis as well as in the posthatched animal. When investigating the colocalization of glycine and GABA immunoreactivities, perikarya immunostained for both amino acids were revealed at all developmental stages from E8 onward, and the proportions of glycine- and GABA-immunoreactive neurons that were also immunostained for the other amino acid were remarkably constant during development. The characteristic features of the development of the investigated putative inhibitory spinal interneurons are discussed and correlated with previous neuroanatomical and physiological studies.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Comparative Neurology. - 362 : 4 (1995), p. 583-596. -
További szerzők:O'Donovan, Michael Antal Miklós (1951-) (orvos, anatómus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM029077
Első szerző:O'Donovan, Michael
Cím:Development of spinal motor networks in the chick embryo / Michael O'Donovan, Evelyne Sernagor, Gerald Sholomenko, Stephen Ho, Miklos Antal, Wayne Yee
Dátum:1992
Megjegyzések:We have examined the cellular and synaptic mechanisms underlying the genesis of alternating motor activity in the developing spinal cord of the chick embryo. Experiments were performed on the isolated lumbosacral cord maintained in vitro. Intracellular and whole cell patch clamp recordings obtained from sartorius (primarily a hip flexor) and femorotibialis (a knee extensor) motoneurons showed that both classes of cell are depolarized simultaneously during each cycle of motor activity. Sartorius motoneurons generally fire two bursts/cycle, whereas femorotibialis motoneurons discharge throughout their depolarization, with peak activity between the sartorius bursts. Voltage clamp recordings revealed that inhibitory and excitatory synaptic currents are responsible for the depolarization of sartorius motoneurons, whereas femorotibialis motoneurons are activated principally by excitatory currents. Early in development, the dominant synaptic currents in rhythmically active sartorius motoneurons appear to be inhibitory so that firing is restricted to a single, brief burst at the beginning of each cycle. In E7-E13 embryos, lumbosacral motor activity could be evoked following stimulation in the brainstem, even when the brachial and cervical cord was bathed in a reduced calcium solution to block chemical synaptic transmission. These findings suggest that functional descending connections from the brainstem to the lumbar cord are present by E7, although activation of ascending axons or electrical synapses cannot be eliminated. Ablation, optical, and immunocytochemical experiments were performed to characterize the interneuronal network responsible for the synaptic activation of motoneurons. Ablation experiments were used to show that the essential interneuronal elements required for the rhythmic alternation are in the ventral part of the cord. This observation was supported by real-time Fura-2 imaging of the neuronal calcium transients accompanying motor activity, which revealed that a high proportion of rhythmically active cells are located in the ventrolateral part of the cord and that activity could begin in this region. The fluorescence transients in the majority of neurons, including motoneurons, occurred in phase with ventral root or muscle nerve activity, implying synchronized neuronal action in the rhythm generating network. Immunocytochemical experiments were performed in E14-E16 embryos to localize putative inhibitory interneurons that might be involved in the genesis or patterning of motor activity. The results revealed a pattern similar to that seen in other vertebrates with the dorsal horn containing neurons with γ-aminobutyric acid (GABA)-like immunoreactivity and the ventral and intermediate regions containing neurons with glycine-like immunoreactivity.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Journal of Experimental Zoology. - 261 : 3 (1992), p. 261-273. -
További szerzők:Sernagor, Evelyne Sholomenko, Gerald N. Ho, Stephen Yee, Wayne Antal Miklós (1951-) (orvos, anatómus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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