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001-es BibID:	BIBFORM038685
035-os BibID:	PMID:15388259
Első szerző:	Gyimesi Edit (klinikai biokémikus, vegyész)
Cím:	Staphylococcus aureus bound to complement receptor 1 on human erythrocytes by bispecific monoclonal antibodies is phagocytosed by acceptor macrophages / Edit Gyimesi, Alexander J. Bankovich, Theodore A. Schuman, Joanna B. Goldberg, Margaret A. Lindorfer, Ronald P. Taylor
Dátum:	2004
Megjegyzések:	AbstractAntibiotic resistant strains of Staphylococcus aureus pose a growing threat to public health, and immunotherapy offers potential modalities to combat antibiotic resistance. We prepared bispecific monoclonal antibody complexes (heteropolymers, HP), specific for the primate erythrocyte complement C3b receptor (CR1) and type 5 capsular polysaccharide of the T5 isolate of S. aureus. Fluorescence microscopy and flow cytometry revealed that HP promote binding of S. aureus to human erythrocytes. Incubation of erythrocyte-bound, HP-opsonized S. aureus with human monocyte/macrophages or mouse macrophage cell lines led to transfer, internalization and killing of bacteria by macrophages with little erythrocyte loss. This reaction is similar to the process in which C3b-opsonized substrates, bound to erythrocyte CR1 by immune adherence, are transferred to acceptor phagocytes. Our results provide the basis for development of an in vivo paradigm focused on immunotherapeutic approaches for treatment of infections due to antibiotic resistant bacteria.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban macrophage bispecific antibody staphylococcus aureus cytometry külföldön készült közlemény
Megjelenés:	Immunolgy Letters. - 95 : 2 (2004), p. 185-192. -
További szerzők:	Bankovich, Alexander J. Schuman, Theodore A. Goldberg, Joanna B. Lindorfer, Margaret A. Taylor, Ronald P.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM038698
Első szerző:	Lindorfer, Margaret A.
Cím:	Targeting of Pseudomonas aeruginosa in the bloodstream with bispecific monoclonal antibodies / Margaret A. Lindorfer, Alessandra Nardin, Patricia L. Foley, Michael D. Solga, Alexander J. Bankovich, Edward N. Martin, Andrea L. Henderson, Carol W. Price, Edit Gyimesi, Colin P. Wozencraft, Joanna B. Goldberg, William M. Sutherland, Ronald P. Taylor
Dátum:	2001
ISSN:	1550-6606
Megjegyzések:	AbstractWe examined the ability of a bispecific mAb reagent, consisting of a mAb specific for the primate erythrocyte complement receptor cross-linked with an anti-bacterial mAb, to target bacteria in the bloodstream in an acute infusion model in monkeys. In vitro studies demonstrated a variable level of complement-mediated binding (immune adherence) of Pseudomonas aeruginosa (strain PAO1) to primate E in serum. In vivo experiments in animals depleted of complement revealed that binding of bacteria to E was <1% before administration of the bispecific reagent, but within 5 min of its infusion, >99% of the bacteria bound to E. In complement-replete monkeys, a variable fraction of infused bacteria bound to E. This finding may have significant implications in the interpretation of animal models and in the understanding of bacteremias in humans. Treatment of these complement-replete monkeys with the bispecific reagent led to >99% binding of bacteria to E. Twenty-four-hour survival studies were conducted; several clinical parameters, including the degree of lung damage, cytokine levels, and liver enzymes in the circulation, indicate that the bispecific mAb reagent provides a degree of protection against the bacterial challenge.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban bispecific antibodies Pseudomona aeruginosa külföldön készült közlemény
Megjelenés:	Journal of Immunology. - 167 : 4 (2001), p. 2240-2249. -
További szerzők:	Nardin, Alessandra Foley, Patricia L. Solga, Michael D. Bankovich, Alexander J. Martin, Edward N. Henderson, Andrea L. Price, Carol W. Wozencraft, Colin P. Goldberg, Joanna B. Sutherland, William M. Taylor, Ronald P. Gyimesi Edit (1957-) (klinikai biokémikus, vegyész)
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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