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001-es BibID:BIBFORM038687
Első szerző:Gyimesi Edit (klinikai biokémikus, vegyész)
Cím:Basophil CD63 expression assay on highly sensitized atopic donor leucocytes-a useful method in chronic autoimmune urticaria / E. Gyimesi, S. Sipka, K. Dankó, E. Kiss, B. Hídvégi, M. Gál, J. Hunyadi, B. Irinyi, A. Szegedi
Dátum:2004
ISSN:0007-0963
Megjegyzések:AbstractBACKGROUND: The autoimmune subclass of chronic idiopathic urticaria (CU) has been characterized by the occurrence of biologically relevant IgG antibodies against the IgE molecule or the alpha chain of the high-affinity Fcepsilon receptor (FcepsilonRIalpha) on basophils and mast cells. These antibodies are usually detected by autologous serum skin testing and confirmed by histamine release studies, immunoblotting, or enzyme-linked immunosorbent assay, but not always.OBJECTIVES: To detect autoantibodies to the FcepsilonRIalpha in sera of CU patients by a modified serum-induced basophil activation test measured by flow cytometry (FCM) and to evaluate the relationship between the in vitro functional test, the autologous serum skin test (ASST), and the serum levels of IgE, eosinophil cationic protein (ECP) and antithyroid antibodies.METHODS: Sera of 30 patients with CU and 26 patients with systemic autoimmune diseases (systemic lupus erythematosus, dermatomyositis) were tested for CD63 activation marker expression on basophils by FCM. Leucocytes from two highly sensitized atopic donors (D(A1,) D(A2)) and one non-atopic donor (D(NA)) were incubated with patients' sera and double-labelled with anti-IgE and anti-CD63 antibodies. Subsequently, the percentage of CD63-expressing basophils was determined by using FCM. In all CU patients an ASST was carried out and the serum IgE, and ECP levels and antithyroid antibodies were evaluated.RESULTS: Twelve patients had a positive ASST and 14 patients a positive CD63 expression assay. There was a strong correlation between the ASST and CD63 assay. Sera from patients with systemic autoimmune diseases did not raise positive CD63 expression on basophils. There was a moderate negative correlation between the occurrence of atopic serum markers (IgE, ECP) and the ability of sera to induce CD63 expression on basophil cells of D(A2) (P < 0.05). The female sex was preponderant and antithyroid antibodies were more frequent.CONCLUSIONS: Our new technical observation demonstrates that basophils of highly sensitized atopic donors can be successfully used without priming with IL-3 for the in-vitro flow cytofluorimetric diagnosis of CU. With this investigation the characterization of the autoimmune origin of CU is based on an objective in vitro technique.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
autoimmune urticaria
CD63 assay
basophil
diagnosis
egyetemen (Magyarországon) készült közlemény
Megjelenés:British Journal of Dermatology. - 151 : 2 (2004), p. 388-396. -
További szerzők:Sipka Sándor (1945-) (laboratóriumi szakorvos) Dankó Katalin (1952-2021) (belgyógyász, allergológus és klinikai immunológus) Kiss Emese (1960-) (belgyógyász, immunológus) Hidvégi Bernadett Gál Mónika Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Irinyi Beatrix (1972-) (bőrgyógyász, allergológus) Szegedi Andrea (1964-) (bőrgyógyász)
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DOI
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001-es BibID:BIBFORM023739
Első szerző:Szegedi Andrea (bőrgyógyász)
Cím:Significant correlation between the CD63 assay and the histamine release assay in chronic urticaria / A. Szegedi, B. Irinyi, M. Gál, J. Hunyadi, K. Dankó, E. Kiss, S. Sipka, G. Szegedi, E. Gyimesi
Dátum:2006
Megjegyzések:Background Antibodies directed to the a subunit of the high affinity IgE receptorand the IgE molecule are proposed to be of pathogenetic relevance in a group ofpatients with chronic urticaria (CU). The diagnosis of autoimmune chronic urticaria(ACU) is difficult; the autologous serum skin test (ASST) seems to be a usefulscreening test, but reliable, additional confirmatory methods are needed.Objectives To assess the diagnostic value of a modified serum-induced basophilactivation test, the CD63 expression assay, in the diagnosis of ACU by comparingthe results of the CD63 assay with the results of the histamine release (HR) test,the ASST and serum levels of soluble CD40 ligand (sCD40L).Methods Using basophils from an atopic (DA) and a nonatopic (DNA) donor theactivity of sera of 72 patients with CU were measured in HR assay by enzymelinkedimmunosorbent assay and in CD63 expression assay by flow cytometry.An ASST was carried out in all patients; in 30 of the 72 patients sCD40L wasdetected and correlations were derived between the different assays. Sera of 20normal controls and 26 patients with systemic autoimmune diseases were alsotested in the HR assay and in the CD63 expression assay.Results Histamine-releasing activity was detected in the sera of 51% (DA) and 32%(DNA) of CU patients and 57% (DA) and 28% (DNA) of sera upregulated CD63expression on the surface of basophils from the different donors. There was asignificant correlation between the HR and the CD63 assays carried out on bothdonors, but the ASST showed a strong correlation with the HR assay only for basophilsfrom the DA. The serum level of sCD40L was significantly higher inpatients with CU compared with controls, but the difference between the autoimmuneand the nonautoimmune groups was not significant.Conclusions The CD63 expression assay seems to be a reliable functional test in thediagnosis of ACU, particularly if highly sensitive donor basophils are used, butthe determination of the sCD40L serum level was not sufficient to differentiate
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
CD63 assay
chronic urticaria
flow cytometry
Megjelenés:British Journal of Dermatology. - 155 : 1 (2006), p. 67-75. -
További szerzők:Irinyi Beatrix (1972-) (bőrgyógyász, allergológus) Gál Mónika Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Dankó Katalin (1952-2021) (belgyógyász, allergológus és klinikai immunológus) Kiss Emese (1960-) (belgyógyász, immunológus) Sipka Sándor (1945-) (laboratóriumi szakorvos) Szegedi Gyula (1936-2013) (belgyógyász, immunológus) Gyimesi Edit (1957-) (klinikai biokémikus, vegyész)
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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