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001-es BibID:	BIBFORM040315
035-os BibID:	PMID:20147564
Első szerző:	Pinto, Vitor
Cím:	Multisegmental A[delta]- and C-fiber input to neurons in lamina I and the lateral spinal nucleus / Vitor Pinto, Peter Szucs, Deolinda Lima, Boris V. Safronov
Dátum:	2010
ISSN:	0270-6474
Megjegyzések:	Spinal lamina I and the lateral spinal nucleus (LSN) receive and integrate nociceptive primary afferent inputs to project through diverse ascending pathways. The pattern of the afferent supply of individual lamina I and LSN neurons through different segmental dorsal roots is poorly understood. Therefore, we recorded responses of lamina I and LSN neurons in spinal segments L4 and L3 to stimulation of six ipsilateral dorsal roots (L1-L6). The neurons were viewed through the overlying white matter in the isolated spinal cord preparation using the oblique infrared LED illumination technique. Orientation of myelinated fibers in the white matter was used as a criterion to distinguish between the LSN and lamina I. Both types of neurons received mixed (monosynaptic and polysynaptic) excitatory Adelta- and C-fiber input from up to six dorsal roots, with only less than one-third of it arising from the corresponding segmental root. The largest mixed input arose from the dorsal root of the neighboring caudal segment. Lamina I and LSN neurons could fire spikes upon the stimulation of up to six different dorsal roots. We also found that individual lamina I neurons can receive converging monosynaptic Adelta- and/or C-fiber inputs from up to six segmental roots. This study shows that lamina I and LSN neurons function as intersegmental integrators of primary afferent inputs. We suggest that broad monosynaptic convergence of Adelta- and C-afferents onto a lamina I neuron is important for the somatosensory processing.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	Journal of Neuroscience. - 30 : 6 (2010), p. 2384-2395. -
További szerzők:	Szűcs Péter (1974-) (kutatóorvos) Lima, Deolinda Safronov, Boris V.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM004505
Első szerző:	Pinto, Vitor
Cím:	Monosynaptic convergence of C- and A(delta)-afferent fibres from different segmental dorsal roots on to single substantia gelatinosa neurones in the rat spinal cord / Vitor Pinto, Peter Szűcs, Victor A. Derkach, Boris V. Safronov
Dátum:	2008
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	The Journal of Physiology (London). - 586 : 17 (2008), p. 4165-4177. -
További szerzők:	Szűcs Péter (1974-) (kutatóorvos) Derkach, Victor A. Safronov, Boris V.
Internet cím:	elektronikus változat DOI
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001-es BibID:	BIBFORM040318
035-os BibID:	PMID:19014968
Első szerző:	Szűcs Péter (kutatóorvos)
Cím:	Advanced technique of infrared LED imaging of unstained cells and intracellular structures in isolated spinal cord, brainstem, ganglia and cerebellum / Péter Szűcs, Vitor Pinto, Boris V. Safronov
Dátum:	2009
ISSN:	0165-0270
Megjegyzések:	Light-emitting diodes (LEDs) have recently been used for the imaging of unstained living cells in the whole brain and spinal cord preparations, in which one cut was done to remove the overlying white matter. Here we show that in many cases the neurones can be visualized through the white matter in an intact nervous tissue (rats P0-P36 and mice P0-P2). We used an upright microscope with a water immersion objective and a powerful infrared LED (emission peak, 850 nm; maximum radiant intensity, 270 mW/sr) as a source of oblique illumination. In the isolated spinal cord, we were able to visualize lamina I and II neurones as well as motoneurones. In the brainstem, the neurones from the superficial nuclei were successfully viewed. In the sensory ganglion, we obtained images of unstained cells as well as intracellular structures, like endoplasmic reticulum, nucleus and nucleolus. In isolated cerebellum, parallel fibers, Purkinje and granule cells were viewed. Whole-cell recordings were done to fill spinal lamina I neurones, motoneurones and brainstem neurones with biocytin for detailed 2D-3D reconstruction of their dendritic and axonal arbores. Our imaging technique also allowed labelling individual intact neurones by injecting biocytin through the extracellular cell-attached pipette. This imaging technique opens broad possibilities for functional studies of neurones with completely preserved anatomical structures and synaptic inputs. We also show that the application of oblique infrared LED illumination allows a construction of a simple digital videomicroscope for the high-quality living cell imaging in intact nervous tissue.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal of Neuroscience Methods. - 177 : 2 (2009), p. 369-380. -
További szerzők:	Pinto, Vitor Safronov, Boris V.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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