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001-es BibID:	BIBFORM040313
035-os BibID:	PMID:20876196
Első szerző:	Luz, Liliana L.
Cím:	Monosynaptic excitatory inputs to spinal lamina I anterolateral-tract-projecting neurons from neighbouring lamina I neurons / Liliana L. Luz, Peter Szucs, Raquel Pinho, Boris V. Safronov
Dátum:	2010
ISSN:	0022-3751
Megjegyzések:	Spinal lamina I receives nociceptive primary afferent input to project through diverse ascending pathways, including the anterolateral tract (ALT). Large projection neurons (PNs) form only a few per cent of the cell population in this layer, and little is known about their local input from other lamina I neurons. We combined single-cell imaging in the isolated spinal cord, paired recordings, 3-D reconstructions of biocytin-labelled neurons and computer simulations to study the monosynaptic input to large ALT-PNs from neighbouring (somata separated by less than 80 m) large lamina I neurons. All 11 connections identified were excitatory. We have found that an axon of a presynaptic neuron forms multiple synapses on an ALT-PN, and both Ca(2+)-permeable and Ca(2+)-impermeable AMPA receptors are involved in transmission. The monosynaptic EPSC latencies (1-12 ms) are determined by both post- and presynaptic factors. The postsynaptic delay, resulting from the electrotonic EPSC propagation in the dendrites of an ALT-PN, could be 4 ms at most. The presynaptic delay, caused by the spike propagation in a narrow highly branched axon of a local-circuit neuron, can be about 10 ms for neighbouring ALT-PNs and longer for more distant neurons. In many cases, the EPSPs evoked by release from a lamina I neuron were sufficient to elicit a spike in an ALT-PN. Our data show that ALT-PNs can receive input from both lamina I local-circuit neurons and other ALT-PNs. We suggest that lamina I is a functionally interconnected layer. The intralaminar network described here can amplify the overall output from the principal spinal nociceptive projection area.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	Journal of Physiology. - 588 : 22 (2010), p. 4489-4505. -
További szerzők:	Szűcs Péter (1974-) (kutatóorvos) Pinho, Raquel Safronov, Boris V.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM040623
Első szerző:	Szűcs Péter (kutatóorvos)
Cím:	Axon diversity of lamina I local-circuit neurons in the lumbar spinal / Szucs Peter, Luz Liliana, Pinho Raquel, Aguiar Paulo, Antal Zsofia, Tiong Sheena, Todd Andrew J., Safronov Boris
Dátum:	2013
Megjegyzések:	Spinal lamina I is a key area for relaying and integrating information from nociceptive primary afferents with various other sources of inputs. While lamina I projection neurons have been intensively studied, much less attention has been given to local-circuit neurons (LCNs), which form the majority of the lamina I neuronal population. In this work the infrared light-emitting diode (IR-LED) oblique illumination technique was used to visualize and label LCNs, allowing reconstruction and analysis of their dendritic and extensive axonal trees. We show that the majority of lamina I neurons with locally branching axons fall into the multipolar (with ventrally protruding dendrites) and flattened (dendrites limited to lamina I) somatodendritic categories. Analysis of their axons revealed that the initial myelinated part gives rise to several unmyelinated small-diameter branches that have a high number of densely packed large varicosities and an extensive rostrocaudal (2-3 segments), mediolateral and dorsoventral (reaching laminae III-IV) distribution. The extent of the axon and the occasional presence of long solitary branches suggest that LCNs may also form short and long propriospinal connections. We also found that the distribution of axon varicosities and terminal field locations show substantial heterogeneity and that a substantial portion of LCNs is inhibitory. Our observations indicate that LCNs of lamina I form intersegmental as well as interlaminar connections and may govern large numbers of neurons, providing anatomical substrate for rostrocaudal "processing units" in the dorsal horn.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Comparative Neurology. - 521 : 12 (2013), p. 2719-2741. -
További szerzők:	Luz, Liliana L. Pinho, Raquel Aguiar, Paulo Antal Zsófia (1985-) (orvos) Tiong, Sheena Todd, Andrew J. Safronov, Boris V.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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Saját polcon: