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001-es BibID:	BIBFORM114946
Első szerző:	Fésüs Adina (gyógyszerész)
Cím:	Evaluation of the antioxidant effect of kd15 and kd36 newly synthetized molecules / Adina Fésüs, Anita Kónya-Ábrahám, Éva Sipos, Kitti Szőke, István Bak, Attila Kiss-Szikszai, István Lekli
Dátum:	2023
Megjegyzések:	KD molecules are national developed halogen-containing chromone (back-bone structure of flavonoids) derivatives with antioxidant effect. In this study our aim was to investigate whether the pretreatment with KD15 and KD36 molecules have a protective effect against hypoxia-reoxygenation (H/R) induced acute injury on cardiac myocytes. In our series of experiments H9c2 rat cardiomyoblast cells were treated for 24 hours and the Ic50 value of the molecules was determined. After that, we investigated the cells viability in the presence of H2O2 using MTT assay. The concentrations of 3 and 10 ?M proved to be the most effective. Subsequently, these concentrations were used to pretreat cardiomyocytes, followed by four/three hours of H/R. Cell viability was determined by trypan blue (dye) exclusion test. Furthermore, lactate dehydrogenase (LDH) assay was used to confirm these results. Western blot analysis was used to examine the expression levels of the proteins involved in apoptosis and autophagy. Our findings demonstrate that the pretreatment with both molecules significantly improved the cardiomyocytes viability after H/R. However, the 10 ?M concentration proved to be more effective for both molecules. Moreover, the KD36 molecule was less cytotoxic. Furthermore, we found a significant decrease in the level of the oxidative stress caused by H/R, leading to an increased release of LDH. At the same time, the activities of cytoprotective antioxidant enzymes, heme oxygenase-1 (HO-1), superoxide dismutase (SOD), and catalase (CAT) were also increased. Overall, we found that KD15 and KD36 molecules may have a beneficial effect in prevention of H/R-induced cytotoxicity through the reduction of oxidative stress.
Tárgyszavak:	Orvostudományok Gyógyszerészeti tudományok előadáskivonat könyvrészlet
Megjelenés:	FAMÉ 2023 Mátraháza 7-9 June 2023 : Programme Oral and Poster Abstract / org. Hungarian Society For Experimental And Clinical Pharmacology, Hungarian Physiological Society, Hungarian Society For Microcirculation And Vascular Biology . - p. 223.
További szerzők:	Ábrahám Anita (1982-) (vegyész) Sipos Éva (1989-) (gyógyszerész) Szőke Kitti (1989-) (gyógyszerész) Bak István (1975-) (vegyész, analitikus, farmakológus) Kiss-Szikszai Attila (1975-) (vegyész) Lekli István (1981-) (gyógyszerész)
Pályázati támogatás:	NKFI-143360 Egyéb TKP2021-EGA-18 Egyéb
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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