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001-es BibID:BIBFORM004837
Első szerző:Hajas György (biológus)
Cím:New phenotypic, functional and electrophysiological characteristics of KG-1 cells / György Hajas, Emese Zsiros, Tünde László, Péter Hajdú, Sándor Somodi, Bence Réthi, Péter Gogolák, Katalin Ludányi, György Panyi, Éva Rajnavölgyi
Dátum:2004
Megjegyzések:Myeloid dendritic cells (DC) are representatives of a rare and phenotypically diverse population of professional antigen presenting cells possessing high functional heterogeneity and flexibility. Here we studied the phenotypic, functional and electrophysiological characteristics of KG-1 cells, an erythroleukemia model cell line, which shares morphological and physiological similarities with immature and mature myeloid DC. We compared the expression of internalizing receptors and other cell surface molecules, antigen uptake and migration of unstimulated and activated KG-1 cells with the characteristics of immature and mature DC. Unstimulated KG-1 cells were less potent in capturing extracellular materials than immature DC. In contrast to monocyte-derived DC KG-1 cells stimulated by PMA and ionomycin ceased to migrate along the MIP-3beta chemokine gradient despite their high expression of CCR7 chemokine receptor and MDR, a transporter implicated in DC migration. Moreover, we determined the ion channel repertoire of KG-1 cells before and after treatment with PMA and ionomycin by using the patch-clamp technique. We found that both unstimulated and activated KG-1 cells expressed time- and voltage-independent, ChTx sensitive intracellular Ca(2+)-gated potassium conductance suggesting the presence of K(Ca) channels in their membranes. Based on our results we propose that KG-1 cells resemble myeloid DC but also possess unique phenotypic, functional and electrophysiological characteristics.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analogs and derivatives
Animals
Calcium
Cell Line
Cell Movement
Cells
Dendritic Cells
Dextrans
Fluorescein
Fluorescein-5-isothiocyanate
Humans
Hungary
immunology
Ionomycin
Isoquinolines
Leukemia,Erythroblastic,Acute
metabolism
Patch-Clamp Techniques
physiology
Potassium
Research
Support
Tumor Cells,Cultured
Megjelenés:Immunology Letters. - 92 : 1-2 (2004), p. 97-106. -
További szerzők:Zsíros Emese (1980-) (orvos) László Tünde Hajdu Péter (1975-) (biofizikus) Somodi Sándor (1977-) (belgyógyász) Réthi Bence (1973-) (biológus, immunológus) Gogolák Péter (1968-) (biológus, immunológus) Ludányi Katalin (1975-) (immunológus) Panyi György (1966-) (biofizikus) Rajnavölgyi Éva (1950-) (immunológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM037603
Első szerző:Varga Zoltán (biofizikus, szakfordító)
Cím:Potassium channel expression in human CD4(+) regulatory and naive T cells from healthy subjects and multiple sclerosis patients / Zoltan Varga, Tunde Csepany, Ferenc Papp, Akos Fabian, Peter Gogolak, Agnes Toth, Gyorgy Panyi
Dátum:2009
ISSN:0165-2478
Megjegyzések:The membrane potential of human T cells is regulated by two potassium channels: the voltage-gatedKV1.3 and the Ca2+-activated KCa3.1. These two channels are essential for efficient antigenic activation andproliferation of T cells and are expressedat different levels in naïve, centralmemory and effectormemory Tcells. This provides the opportunity to inhibit the proliferation of the targeted subtype by channel-specificblocking compounds. Regulatory T cells (Tregs) also represent a unique subtype of T cells that performhighly specialized tasks in controlling immune responses, which raises the possibility that they too havea distinctive channel expression pattern. Using whole-cell patch-clamp we tested this hypothesis anddetermined the ion channel expression of CD4+CD25hiCD127lo regulatory and CD4+CD25loCD127hi naïveT cells from the peripheral blood of healthy volunteers and multiple sclerosis (MS) patients sorted by flowcytometry. We have found that naïve and Treg cells from healthy controls expressed equal numbers ofKV1.3 channels, while Tregs had a greater membrane surface as assessed by capacitance measurements,and consequentially lower channel density than naïve cells, indicating an "incomplete activation state"of Tregs. In contrast, Tregs from MS patients had fewer KV1.3 channels than naïve cells and there wasno difference in the membrane capacitance or channel density between the two subtypes of cells. Theexpression level of KCa3.1 channels was similar in all cell subsets. The observed differences in KV1.3channel expression density may contribute to the varying responses upon antigenic stimulation by thesecell types in health and disease.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Regulatory T cell
Potassium channel
Multiple sclerosis
Cell capacitance
Channel density
egyetemen (Magyarországon) készült közlemény
Megjelenés:Immunology Letters 124 : 2 (2009), p. 95-101. -
További szerzők:Csépány Tünde (1956-) (neurológus, pszichiáter) Papp Ferenc (1979-) (biofizikus) Fábián Ákos István (1982-) (aneszteziológus) Gogolák Péter (1968-) (biológus, immunológus) Tóth Ágnes (1983-) (biofizikus) Panyi György (1966-) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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