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1.

001-es BibID:BIBFORM065577
Első szerző:Gogolák Péter (biológus, immunológus)
Cím:Mapping of a Protective Helper T Cell Epitope of Human Influenza A Virus Hemagglutinin / Péter Gogolák, Ágnes Simon, Attila Horváth, Bence Réthi, István Simon, Katalin Berkics, Éva Rajnavölgyi, Gábor K. Tóth
Dátum:2000
ISSN:0006-291X
Megjegyzések:The synthetic peptide comprising the 317-341 region of human influenza A virus (H1N1 subtype) hemagglutinin elicits peptide-specific antibody and helper T cell responses and confers protection against lethal virus infection. Molecular mapping of the 317-329 region, which encompasses the epitope recognized by peptide-specific T cells, revealed that the minimal size required for T cell activation was the 317-326 segment. The most likely peptide alignment, which placed 320Leu to pocket 1 of the I-E(d) peptide binding groove, was predicted by molecular mechanics calculations performed with the parental and with the Ala-substituted analogs. In line with the prediction data, the results of the peptide binding assay, where the relative binding efficiency to I-E(d) molecules expressed on the surface of antigen-presenting cells was monitored, identified the 320-326 core sequence interacting with the major histocompatibility class II peptide binding groove. Functional analysis of Ala-substituted variants by functional assays and by calculating the surface-accessible areas of the single peptidic amino acids in the I-E(d)-peptide complexes demonstrated that 324Pro is a primary contact residue for the T cell receptor. Our results show that this type of analysis offers a suitable tool for molecular mapping of helper T cell epitopes and thus provides valuable data for subunit vaccine design.Copyright 2000 Academic Press.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical And Biophysical Research Communications 270 : 1 (2000), p. 190-198. -
További szerzők:Simon Ágnes (1969-) (laboratóriumi szakorvos) Horváth Attila (orvos) Réthi Bence (1973-) (biológus, immunológus) Simon István Berkics Katalin Rajnavölgyi Éva (1950-) (immunológus) Tóth Gábor K.
Pályázati támogatás:T022540
OTKA
T030826
OTKA
T030566
OTKA
FKFP 0186/1999
Egyéb
AKP 98-13 3,3
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM057877
Első szerző:Gogolák Péter (biológus, immunológus)
Cím:Targeting dendritic cells for priming cellular immune responses / Péter Gogolák, Bence Réthi, György Hajas, Éva Rajnavölgyi
Dátum:2003
ISSN:0952-3499
Megjegyzések:The cardinal role of dendritic cells (DC) in priming adaptive immunity and in orchestrating immune responses against all classes of pathogens and also against tumors is well established. Their unique potential both to maintain self-tolerance and to initiate protective immune responses against foreign and/or dangerous structures is based on the functional diversity and flexibility of these cells. Tissue DC lining antigenic portals such as mucosal surfaces and the skin are specialized to take up a wide array of compounds including proteins, lipids, carbohydrates, glycoproteins, glycolipids and oligonucleotides, particles carrying such structures and apoptotic or necrotic cells. This process is facilitated by specialized receptors with high endocytic capacity, which provides potential targets for delivering designed molecules. The best route for targeting B- and/or T cell epitopes, however, is still the subject of intense investigation. Immature DC, which reside in various tissues, can be activated by pathogens, stress and inflammation or modified metabolic products, which induce mobilization of cells to draining lymph nodes where they act as highly potent professional antigen presenting cells. This is brought about by the ability to present their accumulated intracellular content for both CD4+ helper (Th) and CD8+ cytotoxic/cytolytic T lymphocytes (Tc/CTL). Engulfed proteins are processed intracellularly and their peptide fragments are transported to the cell surface in the context of major histocompatibility complex encoded class I and II molecules for presentation to Th cells and CTLs, respectively. The T cell priming capacity of DC, however, depends not only on antigen presentation but also on other features of DC. Human monocyte-derived DC provide an excellent tool to study the internalizing, antigen-presenting and T cell-activating functions of DC at their immature and activated differentiation states. These biological activities of DC, however, are highly dependent on their migratory potential from the peripheral non-lymphoid tissues to the lymph nodes, on the expression of adhesion molecules, which support the interaction of DC with T lymphocytes, and the cytokines secreted by DC, which polarize immune responses to Th1-mediated cellular or Th2-mediated antibody responses. These results altogether demonstrate that monocyte-derived DC are useful candidates for in vitro or in vivo targeting of antigens to induce efficient adaptive immune responses against pathogens and also against tumors.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal of Molecular Recognition. - 16 : 5 (2003), p. 299-317. -
További szerzők:Réthi Bence (1973-) (biológus, immunológus) Hajas György (1970-) (biológus) Rajnavölgyi Éva (1950-) (immunológus)
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Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM001482
Első szerző:Gogolák Péter (biológus, immunológus)
Cím:Differentiation of CD1a- and CD1a+ monocyte-derived dendritic cells is biased by lipid environment and PPARgamma / Gogolak P., Réthi B., Szatmári I., Nagy L., Lányi Á., Dezső B., Rajnavölgyi É.
Dátum:2007
Megjegyzések:Accumulating data have shown that the microenvironment of dendritic cells modulates subtype differentiation and CD1 expression, but the mechanisms by which exogenous factors confer these effects are poorly understood. Here we describe the dependence of CD1a- monocyte-derived dendritic cell (moDC) development on lipids associated with the expression of peroxisome proliferator-activated receptor-gamma (PPARgamma). We also show the consecutive differentiation of immature CD1a-PPARgamma+ moDCs to CD1a+PPARgamma- cells limited by serum lipoproteins and terminated by proinflammatory cytokines. Immature CD1a- moDCs possess higher internalizing capacity than CD1a+ cells, whereas both activated subtypes have similar migratory potential but differ in their cytokine and chemokine profiles, which translates to distinct T-lymphocyte-polarizing capacities. CD1a+ moDCs stand out by their capability to secrete high amounts of IL-12p70 and CCL1. As lipoproteins skew moDC differentiation toward the generation of CD1a-PPARgamma+ cells and inhibit the development of CD1a+PPARgamma- cells, we suggest that the uptake of lipids results in endogenous PPARgamma agonists that induce a cascade of gene transcription coordinating lipid metabolism, the expression of lipid-presenting CD1 molecules, subtype dichotomy, and function. The presence of CD1a-PPARgamma+ and CD1a+PPARgamma- DCs in lymph nodes and in pulmonary Langerhans cell histiocytosis confirms the functional relevance of these DC subsets in vivo.
Tárgyszavak:Orvostudományok Természettudományok Elméleti orvostudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
dendritic cell
differentiation
CD1a
PPARgamma
lipid
lipoprotein
T cell polarization
Megjelenés:Blood. - 109 : 2 (2007), p. 643-652. -
További szerzők:Réthi Bence (1973-) (biológus, immunológus) Lányi Árpád (1962-) (biológus, immunológus) Dezső Balázs (1951-) (pathológus) Rajnavölgyi Éva (1950-) (immunológus) Szatmári István (1971-) (biológus) Nagy László (1966-) (molekuláris sejtbiológus, biokémikus)
Internet cím:elektronikus változat
elektronikus változat
DOI
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4.

001-es BibID:BIBFORM004837
Első szerző:Hajas György (biológus)
Cím:New phenotypic, functional and electrophysiological characteristics of KG-1 cells / György Hajas, Emese Zsiros, Tünde László, Péter Hajdú, Sándor Somodi, Bence Réthi, Péter Gogolák, Katalin Ludányi, György Panyi, Éva Rajnavölgyi
Dátum:2004
Megjegyzések:Myeloid dendritic cells (DC) are representatives of a rare and phenotypically diverse population of professional antigen presenting cells possessing high functional heterogeneity and flexibility. Here we studied the phenotypic, functional and electrophysiological characteristics of KG-1 cells, an erythroleukemia model cell line, which shares morphological and physiological similarities with immature and mature myeloid DC. We compared the expression of internalizing receptors and other cell surface molecules, antigen uptake and migration of unstimulated and activated KG-1 cells with the characteristics of immature and mature DC. Unstimulated KG-1 cells were less potent in capturing extracellular materials than immature DC. In contrast to monocyte-derived DC KG-1 cells stimulated by PMA and ionomycin ceased to migrate along the MIP-3beta chemokine gradient despite their high expression of CCR7 chemokine receptor and MDR, a transporter implicated in DC migration. Moreover, we determined the ion channel repertoire of KG-1 cells before and after treatment with PMA and ionomycin by using the patch-clamp technique. We found that both unstimulated and activated KG-1 cells expressed time- and voltage-independent, ChTx sensitive intracellular Ca(2+)-gated potassium conductance suggesting the presence of K(Ca) channels in their membranes. Based on our results we propose that KG-1 cells resemble myeloid DC but also possess unique phenotypic, functional and electrophysiological characteristics.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analogs and derivatives
Animals
Calcium
Cell Line
Cell Movement
Cells
Dendritic Cells
Dextrans
Fluorescein
Fluorescein-5-isothiocyanate
Humans
Hungary
immunology
Ionomycin
Isoquinolines
Leukemia,Erythroblastic,Acute
metabolism
Patch-Clamp Techniques
physiology
Potassium
Research
Support
Tumor Cells,Cultured
Megjelenés:Immunology Letters. - 92 : 1-2 (2004), p. 97-106. -
További szerzők:Zsíros Emese (1980-) (orvos) László Tünde Hajdu Péter (1975-) (biofizikus) Somodi Sándor (1977-) (belgyógyász) Réthi Bence (1973-) (biológus, immunológus) Gogolák Péter (1968-) (biológus, immunológus) Ludányi Katalin (1975-) (immunológus) Panyi György (1966-) (biofizikus) Rajnavölgyi Éva (1950-) (immunológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM065602
Első szerző:Ludányi Katalin (immunológus)
Cím:Fine-tuning of helper T cell activation and apoptosis by antigen-presenting cells / Katalin Ludanyi, Peter Gogolak, Bence Rethi, Maria Magocsi, Cynthia Detre, Janos Matko Eva Rajnavolgyi
Dátum:2004
ISSN:0898-6568
Megjegyzések:The role of antigen-presenting cells (APC) in regulating helper T cell responses and activation-induced cell death (AICD) was investigated in vitro. T cell activation was monitored by measuring the early rise of intracellular free calcium [Ca+]ic, mRNA and cell surface expression of activation and apoptotic molecules, the production of cytokines and the activation of transcription factors. Our results demonstrate that the unique characteristics of a given APC can modify the threshold, kinetics and magnitude of the T cell response. The rapid and sustained rise of intracellular free calcium correlated well with the extent of cytokine production and the expression of activation molecules. Fas-dependent AICD could be induced by the most potent antigen-presenting cell (2PK3) only. Our results demonstrate that the response and fate of effector/memory CD4+ helper T lymphocytes is highly dependent on the individual properties of the APC they encounter.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
APC
Antigen presentation
TCR
Co-stimulation
T cell signaling
Antigen-specific activation
Helper T cell
AICD
Megjelenés:Cellular Signalling. - 16 : 8 (2004), p. 939-950. -
További szerzők:Gogolák Péter (1968-) (biológus, immunológus) Réthi Bence (1973-) (biológus, immunológus) Magócsi Mária Detre, Cynthia Matkó János (1952-) (biológus) Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:T043420
OTKA
NKFP 00088/2001
Egyéb
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Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:BIBFORM086897
Első szerző:Réthi Bence (biológus, immunológus)
Cím:CD150 (SLAM) modulates TLR and CD40 pathways in monocyte-derived dendritic cells / Réthi Bence, Gogolák Péter, Rajnavölgyi Éva, Terhorst C., Lányi Árpád
Dátum:2005
ISSN:1521-6616
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
folyóiratcikk
Megjelenés:Clinical Immunology. - 115 : Suppl1 (2005), p. S26-S26. -
További szerzők:Gogolák Péter (1968-) (biológus, immunológus) Rajnavölgyi Éva (1950-) (immunológus) Terhorst, Cox Lányi Árpád (1962-) (biológus, immunológus)
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Intézményi repozitóriumban (DEA) tárolt változat
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7.

001-es BibID:BIBFORM065598
Első szerző:Réthi Bence (biológus, immunológus)
Cím:Flow cytometry used for the analysis of calcium signaling induced by antigen-specific T-cell activation / Bence Réthi, Cynthia Detre, Péter Gogolák, Attila Kolonics, Mária Magócsi, Éva Rajnavölgyi
Dátum:2002
ISSN:0196-4763
Megjegyzések:Background: In this study, the effect of antigen-presentingcells (APC), peptide concentration, and CD28 costimulationon calcium signaling, induced by antigen-specificT-cell activation, was studied by flow cytometry.Methods: We used two experimental approaches, whichdiffered in their time scale and in the duration of the Tcell-APC interaction, to measure the increase of intracellularfree calcium levels ([Ca2 ]i) in activated T cells: (1)Fluo-3?loaded T cells were activated by cocentrifugationwith peptide-loaded APC and the kinetics of fluorescenceintensity changes was monitored continuously and (2)peptide-loaded APC and T cells were mixed, cocultured,and the fluorescence intensity was measured at varioustime intervals.Results: The calcium signal of T cells was dependent onthe APC as demonstrated by the ratio of cells exhibitinghigh versus low fluorescence intensity and by the magnitudeof the calcium signal in the activated population.Short-term interaction of T cells with less potent APC orwith efficient APC in the presence of low antigen concentrationresulted in decreased calcium signaling. CD28-mediated costimulation enhanced the magnitude and sustainedthe increase of intracellular calcium levels. In linewith the strong and sustained calcium signals, the activationof the calcium-dependent transcription factors NF-AT,AP-1, and NF-B was induced.Conclusions: Flow cytometric methods, feasible for therapid and flexible analysis of calcium signaling upon antigen-specificT-cell activation, were established. Kinetics ofthe increase of mean fluorescence intensity reflected thecalcium response of the total cell population whereasstatistical analysis of fluorescence intensity at selectedtime points provided information on the activation state ofsingle cells. Cytometry 47:207?216, 2002.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
T-cell activation
antigen-presenting cell
calcium signal
costimulation
Megjelenés:Cytometry. - 47 : 4 (2002), p. 207-216. -
További szerzők:Detre, Cynthia Gogolák Péter (1968-) (biológus, immunológus) Kolonics Attila Magócsi Mária Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:030826
OTKA
NKFP 0186/1999
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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8.

001-es BibID:BIBFORM019938
Első szerző:Réthi Bence (biológus, immunológus)
Cím:SLAM/SLAM interactions inhibit CD40-induced production of inflammatory cytokines in monocyte-derived dendritic cells / Bence Réthi, Péter Gogolák, Istvan Szatmari, Ágota Veres, Erika Erdős, Laszlo Nagy, Éva Rajnavölgyi, Cox Terhorst, Árpád Lányi
Dátum:2006
ISSN:0006-4971
Megjegyzések:Signaling lymphocyte activation molecule (SLAM, CD150, or SLAMF1) is a self-ligand receptor on the surface of activated T- and B-lymphocytes, macrophages, and dendritic cells (DCs). Here we examine the effect of SLAM/SLAM interactions on CD40L-induced CD40 signaling pathways in human DCs. CD40L-expressing L929 cells induced DCs to produce interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and IL-12, which was strongly inhibited by coexpression of SLAM on the surface of the L929 cells. Similarly, transfection of DCs with SLAM strongly reduced CD40L-induced IL-12 production. Furthermore, the negative effect of SLAM/SLAM interactions on CD40L-induced DC activation was also detected in the presence of lipopolysaccharide (LPS). LPS-Induced IL-12 secretion, however, was not inhibited by SLAM engagement. CD40L-activated DCs affected by exposure to SLAM/SLAM engagement were impaired in their ability to induce differentiation of naive T lymphocytes into interferon-gamma (IFN-gamma)-producing T-helper 1 (Th1) effector cells. These inhibitory effects were not the result of a general unresponsiveness of DCs to CD40L, as SLAM/SLAM interactions did not prevent CD40L-induced up-regulation of CD83, CD86, or human leukocyte antigen (HLA)-DQ on the surface of DCs. Taken together, the results indicate that SLAM/SLAM interactions inhibit CD40-induced signal transduction in monocyte-derived dendritic cells, an effect that was not detectable in earlier studies using anti-SLAM monoclonal antibodies.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
linked lymphoproliferative-disease
lymphocytic activation molecule
controls T-cell
measles virus
differential expression
immune-responses
encoding gene
CD150 SLAM
in-vitro
SAP
Megjelenés:Blood. - 107 : 7 (2006), p. 2821-2829. -
További szerzők:Gogolák Péter (1968-) (biológus, immunológus) Szatmári István (1971-) (biológus) Veres Ágota (laboráns) Erdős Erika Nagy László (1966-) (molekuláris sejtbiológus, biokémikus) Rajnavölgyi Éva (1950-) (immunológus) Terhorst, Cox Lányi Árpád (1962-) (biológus, immunológus)
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9.

001-es BibID:BIBFORM046853
035-os BibID:Article ID: e55264
Első szerző:Szabó Attila (molekuláris biológus, immunológus, filozófus)
Cím:The Two-Component Adjuvant IC31(R) Boosts Type I Interferon Production of Human Monocyte-Derived Dendritic Cells via Ligation of Endosomal TLRs / Attila Szabo, Peter Gogolak, Kitti Pazmandi, Katalin Kis-Toth, Karin Riedl, Benjamin Wizel, Karen Lingnau, Attila Bacsi, Bence Rethi, Eva Rajnavolgyi
Dátum:2013
ISSN:1932-6203
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Plos One. - 8 : 2 (2013), p. 1-13. -
További szerzők:Gogolák Péter (1968-) (biológus, immunológus) Pázmándi Kitti Linda (1984-) (molekuláris biológus, immunológus) Kis-Tóth Katalin (1975-) (immunológus) Riedl, Karin Wizel, Benjamin Lingnau, Karen Bácsi Attila (1967-) (immunológus) Réthi Bence (1973-) (biológus, immunológus) Rajnavölgyi Éva (1950-) (immunológus)
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Intézményi repozitóriumban (DEA) tárolt változat
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10.

001-es BibID:BIBFORM034344
Első szerző:Szabó Attila (molekuláris biológus, immunológus, filozófus)
Cím:RLR-mediated production of interferon-beta by a human dendritic cell subset and its role in virus-specific immunity / Szabo, A., Bene, K., Gogolak, P., Rethi, B., Lanyi, A., Jankovich, I., Dezso, B., Rajnavolgyi, E.
Dátum:2012
ISSN:0741-5400
Megjegyzések:Cytosolic RIG-I-like helicases (RLR) are PRRs involved in type I IFN production and antiviral immunity. This study focuses to the comparison of the expression, function, and signaling cascades associated to RLR in the previously identified CD14(-)DC-SIGN(+)PPARγ(low)CD1a(+) and CD14(low)DC-SIGN(+)PPARγ(high)CD1a(-) human moDC subsets. Our results revealed that the expression of RLR genes and proteins as well as the activity of the coupled signaling pathways are significantly higher in the CD1a(+) subset than in its phenotypically and functionally distinct counterpart. Specific activation of RLR in moDCs by poly(I:C) or influenza virus was shown to induce the secretion of IFN-beta via IRF3, whereas induction of proinflammatory cytokine responses were predominantly controlled by TLR3. The requirement of RLR-mediated signaling in CD1a(+) moDCs for priming naïve CD8(+) T lymphocytes and inducing influenza virus-specific cellular immune responses was confirmed by RIG-I/MDA5 silencing, which abrogated these functions. Our results demonstrate the subset-specific activation of RLR and the underlying mechanisms behind its cytokine secretion profile and identify CD1a(+) moDCs as an inflammatory subset with specialized functional activities. We also provide evidence that this migratory DC subset can be detected in human tonsil and reactive LNs.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Molekuláris Medicina
Megjelenés:Journal of Leukocyte Biology. - 92 : 1 (2012), p. 159-169. -
További szerzők:Bene Krisztián (1986-) (Biológus) Gogolák Péter (1968-) (biológus, immunológus) Réthi Bence (1973-) (biológus, immunológus) Lányi Árpád (1962-) (biológus, immunológus) Jankovics István Dezső Balázs (1951-) (pathológus) Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
Jelátviteli kapcsolatok ős- és dendritikus sejt altípusokban
Collaboration of signalling pathways in stem- and dendritic cell subsets
NK 101538
OTKA
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Intézményi repozitóriumban (DEA) tárolt változat
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