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001-es BibID:	BIBFORM004837
Első szerző:	Hajas György (biológus)
Cím:	New phenotypic, functional and electrophysiological characteristics of KG-1 cells / György Hajas, Emese Zsiros, Tünde László, Péter Hajdú, Sándor Somodi, Bence Réthi, Péter Gogolák, Katalin Ludányi, György Panyi, Éva Rajnavölgyi
Dátum:	2004
Megjegyzések:	Myeloid dendritic cells (DC) are representatives of a rare and phenotypically diverse population of professional antigen presenting cells possessing high functional heterogeneity and flexibility. Here we studied the phenotypic, functional and electrophysiological characteristics of KG-1 cells, an erythroleukemia model cell line, which shares morphological and physiological similarities with immature and mature myeloid DC. We compared the expression of internalizing receptors and other cell surface molecules, antigen uptake and migration of unstimulated and activated KG-1 cells with the characteristics of immature and mature DC. Unstimulated KG-1 cells were less potent in capturing extracellular materials than immature DC. In contrast to monocyte-derived DC KG-1 cells stimulated by PMA and ionomycin ceased to migrate along the MIP-3beta chemokine gradient despite their high expression of CCR7 chemokine receptor and MDR, a transporter implicated in DC migration. Moreover, we determined the ion channel repertoire of KG-1 cells before and after treatment with PMA and ionomycin by using the patch-clamp technique. We found that both unstimulated and activated KG-1 cells expressed time- and voltage-independent, ChTx sensitive intracellular Ca(2+)-gated potassium conductance suggesting the presence of K(Ca) channels in their membranes. Based on our results we propose that KG-1 cells resemble myeloid DC but also possess unique phenotypic, functional and electrophysiological characteristics.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analogs and derivatives Animals Calcium Cell Line Cell Movement Cells Dendritic Cells Dextrans Fluorescein Fluorescein-5-isothiocyanate Humans Hungary immunology Ionomycin Isoquinolines Leukemia,Erythroblastic,Acute metabolism Patch-Clamp Techniques physiology Potassium Research Support Tumor Cells,Cultured
Megjelenés:	Immunology Letters. - 92 : 1-2 (2004), p. 97-106. -
További szerzők:	Zsíros Emese (1980-) (orvos) László Tünde Hajdu Péter (1975-) (biofizikus) Somodi Sándor (1977-) (belgyógyász) Réthi Bence (1973-) (biológus, immunológus) Gogolák Péter (1968-) (biológus, immunológus) Ludányi Katalin (1975-) (immunológus) Panyi György (1966-) (biofizikus) Rajnavölgyi Éva (1950-) (immunológus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM065602
Első szerző:	Ludányi Katalin (immunológus)
Cím:	Fine-tuning of helper T cell activation and apoptosis by antigen-presenting cells / Katalin Ludanyi, Peter Gogolak, Bence Rethi, Maria Magocsi, Cynthia Detre, Janos Matko Eva Rajnavolgyi
Dátum:	2004
ISSN:	0898-6568
Megjegyzések:	The role of antigen-presenting cells (APC) in regulating helper T cell responses and activation-induced cell death (AICD) was investigated in vitro. T cell activation was monitored by measuring the early rise of intracellular free calcium [Ca+]ic, mRNA and cell surface expression of activation and apoptotic molecules, the production of cytokines and the activation of transcription factors. Our results demonstrate that the unique characteristics of a given APC can modify the threshold, kinetics and magnitude of the T cell response. The rapid and sustained rise of intracellular free calcium correlated well with the extent of cytokine production and the expression of activation molecules. Fas-dependent AICD could be induced by the most potent antigen-presenting cell (2PK3) only. Our results demonstrate that the response and fate of effector/memory CD4+ helper T lymphocytes is highly dependent on the individual properties of the APC they encounter.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban APC Antigen presentation TCR Co-stimulation T cell signaling Antigen-specific activation Helper T cell AICD
Megjelenés:	Cellular Signalling. - 16 : 8 (2004), p. 939-950. -
További szerzők:	Gogolák Péter (1968-) (biológus, immunológus) Réthi Bence (1973-) (biológus, immunológus) Magócsi Mária Detre, Cynthia Matkó János (1952-) (biológus) Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:	T043420 OTKA NKFP 00088/2001 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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