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001-es BibID:	BIBFORM005685
Első szerző:	Magyarics Zoltán (immunológus)
Cím:	Identification of plasmacytoid pre-dendritic cells by one-color flow cytometry for phenotype screening / Zoltan Magyarics, Aniko Csillag, Kitti Pazmandi, Eva Rajnavolgyi, Attila Bacsi
Dátum:	2008
Megjegyzések:	Plasmacytoid pre-dendritic cells (pDCs) are able to prime and polarize naive T-cells, while also having an important effector function in antiviral immunity through the rapid and robust production of interferon-alpha. The main setback of pDCs investigation is the rarity and ex vivo fragility of these cells. Relative simple, reliable, and accurate methods for phenotypic analysis and functional studies of pDCs without isolation would be a great deal of interest. Fresh whole blood samples were analyzed by two-color and one-color flow cytometric pDC-identification assays. The changes in the surface expression of CD62L and HLA-DQ on pDCs in whole blood samples after 24-h treatment with imiquimod, a toll-like receptor 7 agonist, were analyzed. Our data demonstrate that the identification of pDCs in peripheral blood samples can be achieved by using only one fluorescent channel for blood dendritic cell antigen (BDCA)-4 staining combined with the light scatter parameters, thus leaving the other channels open for further phenotypic and/or functional analysis. Recently, several lines of evidence supported the involvement of pDCs in the development of several human diseases, so our new one-color identification approach may provide a useful tool for investigation of the pathomechanism of the relevant diseases by using common, 2-laser benchtop cytometers. Copyright 2008 International Society for Analytical Cytology
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban plasmacytoid dendritic cells flow cytometry BDCA-4 phenotypic analysis
Megjelenés:	Cytometry. Part A. - 73 : 3 (2008), p. 254-258. -
További szerzők:	Csillag Anikó (1979-) (immunológus, biológus, angol-magyar szakfordító) Pázmándi Kitti Linda (1984-) (molekuláris biológus, immunológus) Rajnavölgyi Éva (1950-) (immunológus) Bácsi Attila (1967-) (immunológus)
Internet cím:	elektronikus változat DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM065598
Első szerző:	Réthi Bence (biológus, immunológus)
Cím:	Flow cytometry used for the analysis of calcium signaling induced by antigen-specific T-cell activation / Bence Réthi, Cynthia Detre, Péter Gogolák, Attila Kolonics, Mária Magócsi, Éva Rajnavölgyi
Dátum:	2002
ISSN:	0196-4763
Megjegyzések:	Background: In this study, the effect of antigen-presentingcells (APC), peptide concentration, and CD28 costimulationon calcium signaling, induced by antigen-specificT-cell activation, was studied by flow cytometry.Methods: We used two experimental approaches, whichdiffered in their time scale and in the duration of the Tcell-APC interaction, to measure the increase of intracellularfree calcium levels ([Ca2 ]i) in activated T cells: (1)Fluo-3?loaded T cells were activated by cocentrifugationwith peptide-loaded APC and the kinetics of fluorescenceintensity changes was monitored continuously and (2)peptide-loaded APC and T cells were mixed, cocultured,and the fluorescence intensity was measured at varioustime intervals.Results: The calcium signal of T cells was dependent onthe APC as demonstrated by the ratio of cells exhibitinghigh versus low fluorescence intensity and by the magnitudeof the calcium signal in the activated population.Short-term interaction of T cells with less potent APC orwith efficient APC in the presence of low antigen concentrationresulted in decreased calcium signaling. CD28-mediated costimulation enhanced the magnitude and sustainedthe increase of intracellular calcium levels. In linewith the strong and sustained calcium signals, the activationof the calcium-dependent transcription factors NF-AT,AP-1, and NF-B was induced.Conclusions: Flow cytometric methods, feasible for therapid and flexible analysis of calcium signaling upon antigen-specificT-cell activation, were established. Kinetics ofthe increase of mean fluorescence intensity reflected thecalcium response of the total cell population whereasstatistical analysis of fluorescence intensity at selectedtime points provided information on the activation state ofsingle cells. Cytometry 47:207?216, 2002.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban T-cell activation antigen-presenting cell calcium signal costimulation
Megjelenés:	Cytometry. - 47 : 4 (2002), p. 207-216. -
További szerzők:	Detre, Cynthia Gogolák Péter (1968-) (biológus, immunológus) Kolonics Attila Magócsi Mária Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:	030826 OTKA NKFP 0186/1999 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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