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001-es BibID:	BIBFORM086399
Első szerző:	Detre, Cynthia
Cím:	Dual face of ceramide : non-apoptotic Cer-stimuli modulate antigen-specific T-cell activation through blocking plasma membrane ion channels / Detre C., Kiss E., Varga Zoltán, Ludányi Katalin, Pászty K., Enyedi Á., Panyi György, Rajnavölgyi Éva, Matkó János
Dátum:	2005
ISSN:	1742-464X
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt folyóiratcikk
Megjelenés:	Febs Journal. - 272 : Suppl1 (2005), p. 193. -
További szerzők:	Kiss E. Varga Zoltán (1969-) (biofizikus, szakfordító) Ludányi Katalin (1975-) (immunológus) Pászty Katalin Enyedi Ágnes Panyi György (1966-) (biofizikus) Rajnavölgyi Éva (1950-) (immunológus) Matkó János (1952-) (biológus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM005082
Első szerző:	Detre, Cynthia
Cím:	Death or survival : membrane ceramide controls the fate and activation of antigen-specific T-cells depending on signal strength and duration / Detre, C., Kiss, E., Varga, Z., Ludanyi, K., Paszty, K., Enyedi, A., Kovesdi, D., Panyi, G., Rajnavolgyi, E., Matko, J.
Dátum:	2006
ISSN:	898-6568 (Print)
Megjegyzések:	Sphingomyelinase (SMase)-mediated release of ceramide in the plasma membrane of T-lymphocytes induced by different stimuli such as ligation of Fas/CD95, irradiation, stress, inflammation or anticancer drugs primarily involves mitochondrial apoptosis signaling, but under specific conditions non-apoptotic Fas-signaling was also reported. Here we investigated, using a quantitative simulation model with exogenous C2-ceramide (and SMase), the dependence of activation and fate of T-cells on the strength and duration of ceramide accumulation. A murine, influenza virus hemagglutinin-specific T-helper cell (IP12-7) alone or together with interacting antigen presenting B-cells (APC) was used. C2-ceramide induced apoptosis of TH cells above a 'threshold' stimulus (>25 microM in 'strength' or >30 min in duration), while below the threshold C2-ceramide was non-apoptotic, as confirmed by early and late apoptotic markers (PS-translocation, mitochondrial depolarization, caspase-3 activation, DNA-fragmentation). The modest ceramide stimuli strongly suppressed the calcium response and inhibited several downstream signal events (e.g. ERK1/2-, JNK-phosphorylation, CD69 expression or IL-2 production) in TH cells during both anti-CD3 induced and APC-triggered activation. Ceramide moderately affected the Ca2+ -release from internal stores upon antigen-specific engagement of TCR in immunological synapses, while the influx phase was remarkably reduced in both amplitude and rate, suggesting that the major target(s) of ceramide-effects are membrane-proximal. Ceramide inhibited Kv1.3 potassium channels, store operated Ca2+ -entry (SOC) and depolarized the plasma membrane to which contribution of spontaneously formed ceramide channels is possible. The impaired function of these transporters may be coupled to the quantitative, membrane raft-remodeling effect of ceramide and responsible, in a concerted action, for the suppressed activation. Our results suggest that non-apoptotic Fas stimuli, received from previously activated, FasL+ interacting lymphocytes in the lymph nodes, may negatively regulate subsequent antigen-specific T-cell activation and thus modulate the antigen-specific T-cell response.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analogs and derivatives Animals Antigens,CD95 Apoptosis B-Lymphocytes Calcium Caspase 3 Caspases Cell Membrane Cell Survival Cells DNA Fragmentation Human Humans Hungary immunology Inflammation Interleukin-2 Kv1.3 Potassium Channel Lymph Nodes Lymphocyte Activation Lymphocytes Membrane Potentials metabolism Mice pharmacology physiology Potassium Potassium Channels Receptors,Antigen,T-Cell Research Signal Transduction Sphingomyelin Phosphodiesterase Sphingosine Support Synapses T-Lymphocytes T-Lymphocytes, Helper-Inducer Time Factors
Megjelenés:	Cellular Signalling. - 18 : 3 (2006), p. 294-306. -
További szerzők:	Kiss Endre (Budapest) Varga Zoltán (1969-) (biofizikus, szakfordító) Ludányi Katalin (1975-) (immunológus) Pászty Katalin Enyedi Ágnes Kövesdi Dorottya Panyi György (1966-) (biofizikus) Rajnavölgyi Éva (1950-) (immunológus) Matkó János (1952-) (biológus)
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001-es BibID:	BIBFORM065602
Első szerző:	Ludányi Katalin (immunológus)
Cím:	Fine-tuning of helper T cell activation and apoptosis by antigen-presenting cells / Katalin Ludanyi, Peter Gogolak, Bence Rethi, Maria Magocsi, Cynthia Detre, Janos Matko Eva Rajnavolgyi
Dátum:	2004
ISSN:	0898-6568
Megjegyzések:	The role of antigen-presenting cells (APC) in regulating helper T cell responses and activation-induced cell death (AICD) was investigated in vitro. T cell activation was monitored by measuring the early rise of intracellular free calcium [Ca+]ic, mRNA and cell surface expression of activation and apoptotic molecules, the production of cytokines and the activation of transcription factors. Our results demonstrate that the unique characteristics of a given APC can modify the threshold, kinetics and magnitude of the T cell response. The rapid and sustained rise of intracellular free calcium correlated well with the extent of cytokine production and the expression of activation molecules. Fas-dependent AICD could be induced by the most potent antigen-presenting cell (2PK3) only. Our results demonstrate that the response and fate of effector/memory CD4+ helper T lymphocytes is highly dependent on the individual properties of the APC they encounter.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban APC Antigen presentation TCR Co-stimulation T cell signaling Antigen-specific activation Helper T cell AICD
Megjelenés:	Cellular Signalling. - 16 : 8 (2004), p. 939-950. -
További szerzők:	Gogolák Péter (1968-) (biológus, immunológus) Réthi Bence (1973-) (biológus, immunológus) Magócsi Mária Detre, Cynthia Matkó János (1952-) (biológus) Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:	T043420 OTKA NKFP 00088/2001 Egyéb
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001-es BibID:	BIBFORM065598
Első szerző:	Réthi Bence (biológus, immunológus)
Cím:	Flow cytometry used for the analysis of calcium signaling induced by antigen-specific T-cell activation / Bence Réthi, Cynthia Detre, Péter Gogolák, Attila Kolonics, Mária Magócsi, Éva Rajnavölgyi
Dátum:	2002
ISSN:	0196-4763
Megjegyzések:	Background: In this study, the effect of antigen-presentingcells (APC), peptide concentration, and CD28 costimulationon calcium signaling, induced by antigen-specificT-cell activation, was studied by flow cytometry.Methods: We used two experimental approaches, whichdiffered in their time scale and in the duration of the Tcell-APC interaction, to measure the increase of intracellularfree calcium levels ([Ca2 ]i) in activated T cells: (1)Fluo-3?loaded T cells were activated by cocentrifugationwith peptide-loaded APC and the kinetics of fluorescenceintensity changes was monitored continuously and (2)peptide-loaded APC and T cells were mixed, cocultured,and the fluorescence intensity was measured at varioustime intervals.Results: The calcium signal of T cells was dependent onthe APC as demonstrated by the ratio of cells exhibitinghigh versus low fluorescence intensity and by the magnitudeof the calcium signal in the activated population.Short-term interaction of T cells with less potent APC orwith efficient APC in the presence of low antigen concentrationresulted in decreased calcium signaling. CD28-mediated costimulation enhanced the magnitude and sustainedthe increase of intracellular calcium levels. In linewith the strong and sustained calcium signals, the activationof the calcium-dependent transcription factors NF-AT,AP-1, and NF-B was induced.Conclusions: Flow cytometric methods, feasible for therapid and flexible analysis of calcium signaling upon antigen-specificT-cell activation, were established. Kinetics ofthe increase of mean fluorescence intensity reflected thecalcium response of the total cell population whereasstatistical analysis of fluorescence intensity at selectedtime points provided information on the activation state ofsingle cells. Cytometry 47:207?216, 2002.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban T-cell activation antigen-presenting cell calcium signal costimulation
Megjelenés:	Cytometry. - 47 : 4 (2002), p. 207-216. -
További szerzők:	Detre, Cynthia Gogolák Péter (1968-) (biológus, immunológus) Kolonics Attila Magócsi Mária Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:	030826 OTKA NKFP 0186/1999 Egyéb
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