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001-es BibID:	BIBFORM059768
Első szerző:	Chatterjee, Arunima (immunológus)
Cím:	The Impact of ATRA on Shaping Human Myeloid Cell Responses to Epithelial Cell-Derived Stimuli and on T-Lymphocyte Polarization / Arunima Chatterjee, Péter Gogolak, Hervé M. Blottière, Éva Rajnavölgyi
Dátum:	2014
ISSN:	0962-9351
Megjegyzések:	Vitamin A plays an essential role in the maintenance of gut homeostasis but its interplay with chemokines has not been explored so far. Using an in vitro model system we studied the effects of human colonic epithelial cells (Caco2, HT-29, and HCT116) derived inflammatory stimuli on monocyte-derived dendritic cells and macrophages. Unstimulated Caco2 and HT-29 cells secreted CCL19, CCL21, and CCL22 chemokines, which could attract dendritic cells and macrophages and induced CCR7 receptor up-regulation by retinoic-acid resulting in dendritic cell migration. The chemokines Mk, CXCL16, and CXCL7 were secreted by all the 3 cell lines tested, and upon stimulation by IL-1?? or TNF-?? this effect was inhibited by ATRA but+had no impact on CXCL1, CXCL8, and CCL20 secretion in response to IL-1??. In the presence of ATRA the supernatants of these cells induced CD103 expression on monocyte- derived dendritic cells and when conditioned by ATRA and cocultured with CD4 T-lymphocytes they reduced the proportion of Th17 T-cells. However, in the macrophage-T-cell cocultures the number of these effector T-cells was increased. Thus cytokine- activated colonic epithelial cells trigger the secretion of distinct combinations of chemokines depending on the proinflammatory stimulus and are controlled by retinoic acid, which also governs dendritic cell and macrophage responses.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Mediators Of Inflammation. - 2015 (2015), p. 1-14. -
További szerzők:	Gogolák Péter (1968-) (biológus, immunológus) Blottiére, Hervé M. Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:	PEOPLE-2007-1-1-ITN/CROSS-TALK/215553 FP7
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM059770
Első szerző:	Kalló Gergő (molekuláris biológus)
Cím:	Relative quantification of human [béta]-defensins by a proteomics approach based on selected reaction monitoring / Gergő Kalló, Arunima Chatterjee, Márta Tóth, Éva Rajnavölgyi, Adrienne Csutak, József Tőzsér, Éva Csősz
Dátum:	2015
ISSN:	0951-4198
Megjegyzések:	RATIONALE: A targeted proteomics method based on selected reaction monitoring (SRM) is a relevant approach for theanalysis of multiple analytes in biological samples. Defensins are phylogenetically conserved small antimicrobialpeptides contributing to innate host defense and exhibiting low immunogenicity, resistance to proteolysis and a broadrange of antimicrobial activities. The goal of the present study was to develop and optimize SRM-based targetedproteomics methods for the detection of human [béta]-defensins 1?4 in various biological fluids.METHODS: An SRM-based targeted proteomics method was developed and validated for the detection of human?-defensins 1?4. The supernatants of resting and IL-1?-stimulated Caco2, HT-29 and SW-1116 colonic epithelial cells(CEC), cell lysates of CECs and tear samples of human healthy individuals were analyzed and the feasibility of thedeveloped method was validated by ELISA and dot-blot analysis complemented by RT-qPCR.RESULTS: Our results demonstrate that the developed SRM method offers an alternative approach for the cost-effectiveand rapid analysis of human [béta]-defensins in samples with biological relevance.CONCLUSIONS: A semi-quantitative targeted mass spectrometry method was developed and validated for the relativequantification of [béta]-defensins 1?4 in cell culture supernatants and body fluid analyses.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban [béta]-defensin tear relative quantification targeted proteomics SRM MRM
Megjelenés:	Rapid Communications In Mass Spectrometry. - 29 : 18 (2015), p. 1623-1631. -
További szerzők:	Chatterjee, Arunima (1981-) (immunológus) Tóth Márta (1986-) (biológus) Rajnavölgyi Éva (1950-) (immunológus) Csutak Adrienne (1971-) (szemész) Tőzsér József (1959-) (molekuláris biológus, biokémikus, vegyész) Csősz Éva (1977-) (biokémikus, molekuláris biológus)
Pályázati támogatás:	TÁMOP 4.2.4.A/2-11-1-2012-0001 TÁMOP 4.2.2.A- 11/1/KONV-2012-0045 TÁMOP 4.2.2.A-11/1/KONV-2012-0023 Marie-Curie Cross-Talk program FP7-215553 (2007?2013) Egyéb
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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