Találati lista | Tudóstér

001-es BibID:	BIBFORM054136
035-os BibID:	Article ID: e81516
Első szerző:	Hsieh, Yu-Fan
Cím:	Transglutaminase 2 Contributes to Apoptosis Induction in Jurkat T Cells by Modulating Ca2+ Homeostasis via Cross-Linking RAP1GDS1 / Yu-Fan Hsieh, Guang-Yaw Liu, Yi-Ju Lee, Jiann-Jou Yang, Katalin Sándor, Zsolt Sarang, Angela Bononi, Paolo Pinton, László Tretter, Zsuzsa Szondy, Gregory J. Tsay
Dátum:	2013
ISSN:	1932-6203
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Plos One. - 8 : 12 (2013), p. 1-14. -
További szerzők:	Liu, Guang-Yaw Lee, Yi-Ju Yang, Jiann-Jou Sándor Katalin (1987-) (molekuláris biológus, biokémikus-genomikus) Sarang Zsolt (1976-) (mikrobiológus) Bononi, Angela Pinton, Paolo Tretter László Szondy Zsuzsanna (1959-) (molekuláris sejtbiológus, biokémikus) Tsay, Gregory J.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM063958
Első szerző:	Sándor Katalin (molekuláris biológus, biokémikus-genomikus)
Cím:	Transcriptional control of transglutaminase 2 expression in mouse apoptotic thymocytes / Katalin Sándor, Bence Dániel, Bea Kiss, Fruzsina Kovács, Zsuzsa Szondy
Dátum:	2016
ISSN:	1874-9399
Megjegyzések:	Transglutaminase 2 (TGM2) is a ubiquitously expressed multifunctional protein, which participates in various biological processes including thymocyte apoptosis. As a result, the transcriptional regulation of the gene is complex and must depend on the cell type. Previous studies from our laboratory have shown that in dying thymocytes the expression of TGM2 is induced by external signals derived from engulfing macrophages, such as retinoids, transforming growth factor (TGF)-β and adenosine, the latter triggering the adenylate cyclase signaling pathway. The existence of TGF-β and retinoid responsive elements in the promoter region of Tgm2 has already been reported, but the intergenic regulatory elements participating in the regulation of Tgm2 have not yet been identified. Here we used publicly available results from DNase I hypersensitivity analysis followed by deep sequencing and chromatin immunoprecipitation followed by deep sequencing against CCCTC-binding factor, H3K4me3, H3K4me1 and H3K27ac to map a putative regulatory element set for Tgm2 in thymocytes. By measuring eRNA expressions of these putative enhancers in retinoid, rTGF-β or dibutiryl cAMP-exposed thymocytes we determined which of them are functional. By applying ChIP-qPCR against SMAD4, retinoic acid receptor, retinoid X receptor, cAMP response element binding protein, P300 and H3K27ac under the same conditions, we identified two enhancers of Tgm2, which seem to act as integrators of the TGF-β, retinoid and adenylate cyclase signaling pathways in dying thymocytes. Our study describes a novel strategy to identify and characterize the signal specific functional enhancer set of a gene by integrating genome-wide datasets and measuring the production of enhancer specific RNA molecules
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban T cell Apoptosis gene regulation enhancer RNA transglutaminase 2 chip-seq
Megjelenés:	Biochimica et Biophysica Acta (BBA). Gene Regulatory Mechanisms. - 1859 : 8 (2016), p. 964-974. -
További szerzők:	Dániel Bence (1987-) (molekuláris biológus) Kiss Beáta (1984-) (biokémikus, molekuláris biológus) Kovács Fruzsina (2000-) (molekuláris biológus, biotechnológus) Szondy Zsuzsanna (1959-) (molekuláris sejtbiológus, biokémikus)
Pályázati támogatás:	K104228 OTKA NK 105046 OTKA 4.2.2.A-11/1/KONV-2012-0023 TÁMOP
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI Szerző által megadott URL
Borító:
Saját polcon:

001-es BibID:	BIBFORM063955
Első szerző:	Sándor Katalin (molekuláris biológus, biokémikus-genomikus)
Cím:	Adenosine produced from adenine nucleotides through an interaction between apoptotic cells and engulfing macrophages contributes to the appearance of transglutaminase 2 in dying thymocytes / Katalin Sándor, Anna Pallai, Edina Duró, Pascal Legendre, Isabelle Couillin, Tibor Sághy, Zsuzsa Szondy
Dátum:	2017
ISSN:	0939-4451
Megjegyzések:	Transglutaminase 2 (TG2) has been known for along time to be associated with the in vivo apoptosis programof various cell types, including T cells. Though theexpression of the enzyme is strongly induced in mousethymocytes following apoptosis induction in vivo, no significantinduction of TG2 can be detected, when thymocytesare induced to die by the same stimuli in vitro indicatingthat signals arriving from the tissue environment arerequired for the proper in vivo induction of the enzyme.Previous studies from our laboratory have demonstratedthat two of these signals, transforming growth factor-?(TGF-?) and retinoids, are produced by macrophagesengulfing apoptotic cells. However, in addition to TGF-? and retinoids, engulfing macrophages produce adenosineas well. Here, we show that in vitro adenosine, adenosine,and retinoic acid or adenosine, TGF-? and retinoicacids together can significantly enhance the TG2 mRNAexpression in dying thymocytes. The effect of adenosineis mediated via adenosine A2A receptors (A2ARs) andthe A2AR-triggered adenylate cyclase signaling pathway.In accordance, loss of A2ARs in A2AR null mice significantlyattenuates the in vivo induction of TG2 followingapoptosis induction in the thymus indicating that adenosineindeed contributes in vivo to the apoptosis-related appearanceof the enzyme. We also demonstrate that adenosineis produced extracellularly during engulfment of apoptoticthymocytes, partly from adenine nucleotides released viathymocyte pannexin-1 channels. Our data reveal a novelcrosstalk between macrophages and apoptotic cells, inwhich apoptotic cell uptake-related adenosine productioncontributes to the appearance of TG2 in the dyingthymocytes.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Apoptózis makrofág Fagocitózis Adenozin transzglutamináz 2
Megjelenés:	Amino Acids 49 : 3 (2017), p. 671-681. -
További szerzők:	Pallai Anna (1983-) (biokémikus, molekuláris biológus) Duró Edina Legendre, Pascal Couillin, Isabelle Sághy Tibor Szondy Zsuzsanna (1959-) (molekuláris sejtbiológus, biokémikus)
Pályázati támogatás:	T104228 OTKA NK 105046 OTKA 4.2.2.A-11/1/KONV-2012-0023 TÁMOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: