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001-es BibID:	BIBFORM037279
Első szerző:	Csanaky Katalin
Cím:	Changes in PACAP Immunoreactivity in Human Milk and Presence of PAC1 Receptor in Mammary Gland during Lactation / Csanaky Katalin, Banki Eszter, Szabadfi Krisztina, Reglodi Dora, Tarcai Ibolya, Czegledi Levente, Helyes Zsuzsanna, Ertl Tibor, Gyarmati Judit, Szanto Zalan, Zapf Istvan, Sipos Erika, Shioda Seiji, Tamas Andrea
Dátum:	2012
ISSN:	0895-8696
Megjegyzések:	Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence in the nervous system and peripheral organs, including the mammary gland. Previously, we have shown that PACAP38 is present in the human milk at higher levels than in respective blood samples. However, it is not known how PACAP levels and the expression of PAC1 receptor change during lactation. Therefore, the aim of our study was to investigate PACAP38-like immunoreactivity (PACAP38-LI) in human colostrums and transitional and mature milk during lactation and to compare the expression of PAC1 receptors in lactating and non-lactating mammary glands. We found that PACAP38-LI was significantly higher in human colostrum samples than in the transitional and mature milk. PACAP38-LI did not show any significant changes within the first 10-month period of lactation, but a significant increase was observed thereafter, up to the examined 17th month. Weak expression of PAC1 receptors was detected in non-lactating sheep and human mammary glands, but a significant increase was observed in the lactating sheep samples. In summary, the present study is the first to show changes of PACAP levels in human milk during lactation. The presence of PACAP in the milk suggests a potential role in the development of newborn, while the increased expressions of PAC1 receptors on lactating breast may indicate a PACAP38/PAC1 interaction in the mammary gland during lactation.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban sheep, PACAP Egészség- és Környezettudomány
Megjelenés:	Journal Of Molecular Neuroscience. - 48 : 3 (2012), p. 631-637. -
További szerzők:	Bánki Eszter Szabadfi Krisztina (Pécsi Egyetem) Reglődi Dóra (Idegtudományok) Tarcai Ibolya Czeglédi Levente (1977-) (agrármérnök) Helyes Zsuzsanna Ertl Tibor Gyarmati Judit Szántó Zalán Zapf István Sípos Erika Shioda, Seiji Tamás Andrea (Idegtudomány) (Pécs)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Állati eredetű funkcionális élelmiszerek fejlesztése
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM087705
Első szerző:	Kiss Péter (Pécs)
Cím:	Presence of PACAP-38 in mammalian plasma and milk : from guinea pig to humans / Kiss P., Csanaky K., Bánki E., Tamás A., Börzsei R., Márk L., Bagoly T., Bay Cs., Váczy A., Horváth G., Németh J., Czeglédi L., Szauer E., Helyes Zs., Reglődi D.
Dátum:	2010
ISSN:	0231-424X 1588-2683
Megjegyzések:	PACAP is a pleiotropic and multifunctional neuropeptide widely distributed throughout the body. It is involved in the regulation of various physiological and pathophysiological processes, such as reproduction, thermoregulation, motor activity, brain development, neuronal survival, inflammation and pain. Since little is known about its distribution in humans, our aim was to examine PACAP-38 in human plasma. Furthermore, based on the presence of VIP, structurally the closest to PACAP, in milk, and presence of PACAP- and PACAP receptor-immunoreactive elements in the mammary gland, our aim was also to examine PACAP-38 in human milk. For comparative studies, we investigated presence of PACAP in plasma and milk of other species, including goat, sheep, cow and guinea pig. Methods. The presence of PACAP-38 was determined by mass spectrometry in plasma samples from healthy male and female volunteers (age: 20?40), in plasma and milk samples from lactating women (age: 20?35). PACAP concentration was measured with a specific and sensitive radioimmunoassay in human and other mammalian samples. Results. Our results revealed that PACAP-38 is present in human plasma, its concentration is relatively stable in healthy volunteers and it is not significantly altered by gender, age, food intake or the hormonal cycle in females. However, PACAP-38 plasma levels significantly increased in lactating women having 1?6-month-old babies. Moreover, this study is the first which provides evidence for the presence of PACAP-38 in the human milk with levels 5?20-fold greater in the milk whey than in the respective plasma samples. The same ratio was found in all other examined species. Conclusions. Based on the present findings and the literature, several important actions of milk-derived PACAP-38 can be suggested such as mammary gland proliferation, nutrient transfer as well as regulation of growth/differentiation of certain tissues of the neonates.
Tárgyszavak:	Orvostudományok Egészségtudományok idézhető absztrakt folyóiratcikk PACAP-38
Megjelenés:	Acta Physiologica Hungarica. - 97 : 1 (2010), p. 115. -
További szerzők:	Csanaky Katalin Bánki Eszter Tamás Andrea (Idegtudomány) (Pécs) Börzsei Rita Márk László Bagoly Teréz Bay Csaba Váczy Alexandra Horváth Gabriella Németh József (1954-) (vegyész, analitikus) Czeglédi Levente (1977-) (agrármérnök) Szauer Edit Helyes Zsuzsanna Reglődi Dóra (Idegtudományok)
Pályázati támogatás:	K72592 OTKA K73044 OTKA F67830 OTKA 78480 OTKA K75965 OTKA Gedeon Richter Centenary Foundation Egyéb Janos Bolyai Postdoctoral Research Fellowship Egyéb
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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