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1.

001-es BibID:BIBFORM112724
035-os BibID:(cikkazonosító)109974 (WoS)001035781600001 (Scopus)85163192208
Első szerző:Khangembam, Rojesh
Cím:Preliminary Results of the Recombinase Polymerase Amplification Technique for the Detection of Haemonchus contortus from Hungarian Field Samples / Rojesh Khangembam; Nóra Vass; Alison Morrison; Lynsey A Melville; Alistair Antonopoulos; Levente Czeglédi
Dátum:2023
ISSN:0304-4017
Megjegyzések:Haemonchus contortus is a parasitic nematode of small ruminants responsible for significant economic losses and animal health concerns globally. Detection of gastrointestinal nematode (GIN) infection in veterinary practice typically relies on microscopy-based methods such as the faecal egg count and morphological identification of larval culture. However, mixed co-infections are common and species-specific identification is typically time-consuming and expertise-intensive. Compounded by increasing anthelmintic resistance, there is an urgent need to implement the molecular diagnosis of GIN in the livestock industry, preferably in field settings. Advances in isothermal amplification techniques including recombinase polymerase amplification (RPA) assays could improve this. Yet, constraints in RPA kit availability and amplicon detection systems limit the use of this technology in point of care settings. In this study, we present an early-stage, proof-of-concept demonstration of RPA targeting the internal transcribed spacer (ITS2) region of H. contortus. Having tested against eight closely related nematodes and also against five farm isolates in Eastern Hungary, preliminary results derived from a comparative analysis of 3 primer sets showed the assay detects H. contortus DNA and has a limit of detection of 10-5 ng/?l. We also tested an end-result naked eye detection system using various DNA binding dyes, of which EvaGreen® dye was successful for a qualitative RPA detection that could be adaptable at farm sites.
Tárgyszavak:Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Haemonchus
RPA
isothermal amplification
point of care
diagnostic
Megjelenés:Veterinary Parasitology. - 320 (2023), p. 1-20. -
További szerzők:Vass Nóra (1983-) (állatorvos) Morrison, Alison Melville, Lynsey A Antonopoulos, Alistair Czeglédi Levente (1977-) (agrármérnök)
Pályázati támogatás:EFOP-3.6.3-VEKOP-16-2017-00008
EFOP-VEKOP
Internet cím:DOI
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2.

001-es BibID:BIBFORM112321
Első szerző:Khangembam, Rojesh
Cím:Routine microscopy examination of faecal samples as a tool for detection of common gastrointestinal parasites : a preliminary report from two Hungarian farms / Rojesh Khangembam, Levente Czeglédi, Nóra Vass
Dátum:2023
ISSN:1587-1282
Megjegyzések:Gastrointestinal parasitism in ruminant animals is a cause of major economic loss incurred by the livestock industry. Regardless of the frequency of the adopted therapeutic and prophylactic deworming strategies, the parasitic burden in a farm should be assessedregularly. One of the most widely used techniques to do so is the microscopic faecal egg examination and faecal egg counting method. Despite the technique being almost a century old from its first adoption, the principle behind the newer techniques of faecal egg examination is the same. This technique is still being used in routine farm screening and monitoring gastrointestinal parasitic load and faecal egg count reduction testing to assess the anthelmintic efficacy of the drugs used. Thus, the tool remains a choice for preliminary screening for important parasites and the subsequent deworming strategy. Our study here was part of a larger survey on the treatment efficiency as well as a broad epidemiological study of the trichostrongyle parasites in Hungary. We present a preliminary report on the detection of common gastrointestinal parasites from two farms in Hungary, including a species-specific confirmatory microscopy for Haemonchus contortus eggs.
Tárgyszavak:Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény hazai lapban
folyóiratcikk
Trichostrongyle egg
faecal egg count
microscopy technique
small ruminants
Haemonchus contortus
Megjelenés:Agrártudományi Közlemények = Acta agraria Debreceniensis. - 1 (2023), p. 63-66. -
További szerzők:Czeglédi Levente (1977-) (agrármérnök) Vass Nóra (1983-) (állatorvos)
Pályázati támogatás:TKP2020-NKA-04
Egyéb
Internet cím:DOI
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3.

001-es BibID:BIBFORM112318
Első szerző:Khangembam, Rojesh
Cím:Recovery and confirmation of Haemonchus contortus from abomasal contents of roe deer (Capreolus capreolus) in Eastern-Hungary (Biharugra): A diagnostic case study / Rojesh Khangembam, Mariann Tóth, Gabriella Gulyas, Levente Czeglédi, Nóra Vass
Dátum:2023
ISSN:1587-1282
Megjegyzések:Gastrointestinal parasites are ubiquitous. They occur both in wild and domesticated animals. Among such parasites of veterinary importance is the trichostrongyle worms, out of which the Haemonchus contortus species is regarded as the most pathogenic one in the sma ll ruminant industry. The occurrence of this parasite in the sheep flock is now very well documented and an established fact in Europe, a lthough the parasite was original of the warmer climatic region. Studies on the cross-transmission of H. contortus between the wild and domesticated animals are also on the rise although the question of the direction of transmission is still debated. This is an important area that needs to be addressed as it could potentially contribute indirectly to mitigating anthelmintic resistance. Hungary also has reported its share of the occurrence of the parasite, mainly in the sheep flock and a certain population of roe deer. The study presented here is the preliminary results of a diagnostic case study that confirms the presence of H. contortus in wild ruminant deer species that are close to the domesticated sheep population.
Tárgyszavak:Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény hazai lapban
folyóiratcikk
Haemonchus contortus
Roe deer
Hungary
PCR
Megjelenés:Agrártudományi Közlemények = Acta agraria Debreceniensis. - 1 (2023), p. 59-62. -
További szerzők:Tóth Mariann (1994-) (agrármérnök) Gulyás Gabriella (1985-) (agrár) Czeglédi Levente (1977-) (agrármérnök) Vass Nóra (1983-) (állatorvos)
Pályázati támogatás:TKP2020-NKA-04
Egyéb
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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DOI
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4.

001-es BibID:BIBFORM111145
Első szerző:Khangembam, Rojesh
Cím:Detection of Haemonchus contortus eggs using peanut agglutinin (PNA) fluorescence microcopy / Rojesh Khangembam ; Czeglédi Levente ; Nóra Vass
Dátum:2022
Megjegyzések:Haermonchus contortus is one of the most pathogenic nematode parasites of sheep. It feeds on the blood of the host animals and a severely infected animal could carry 3000-5000 adult worms, losing up to 250ml of blood every day. Yet, the diagnosis of nematode parasites still relies mainly on conventional microscopic examination of the parasitic eggs in the faeces of the animal. This parasite usually occurs as a mixed infection and the egg morphology is hard to differentiate from its closely related but less harmful species. Accurate differentiation and diagnosis of the parasite are crucial in reducing unwanted drug usage. Using a lectin (peanut agglutinin) that binds specifically to the egg of H. contortus which could be visualised by fluorescence microscopy. the infection of H. contortus can be established more accurately. This study presents the preliminary results of this technique to help in mapping a haemonchosis prevalence in Hungary.
ISBN:978-615-81413-2-1
Tárgyszavak:Agrártudományok Állatorvosi tudományok előadáskivonat
könyvrészlet
Haemonchus contortus
peanut agglutinin
sheep
Hungary
Megjelenés:XXX Jubilee International Congress of the Hungarian Association for Buiatrics. Proceedings. - p. 221-224. -
További szerzők:Czeglédi Levente (1977-) (agrármérnök) Vass Nóra (1983-) (állatorvos)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM108921
Első szerző:Khangembam, Rojesh
Cím:Proof of concept recombinase polymerase assay (RPA) for the detection of Haemonchus contortus in ruminants / Rojesh Khangembam, Levente Czeglédi, Nóra Vass, Alistair Antonopoulos
Dátum:2022
Tárgyszavak:Agrártudományok Állatorvosi tudományok előadáskivonat
könyvrészlet
Megjelenés:ICOPA 2022 International Congress of Parasitology Online Abstracts. - p. 1269.
További szerzők:Czeglédi Levente (1977-) (agrármérnök) Vass Nóra (1983-) (állatorvos) Antonopoulos, Alistair
Internet cím:Szerző által megadott URL
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6.

001-es BibID:BIBFORM101791
035-os BibID:(cikkazonosító)82 (WoS)000730412700001 (Scopus)85122021532
Első szerző:Khangembam, Rojesh
Cím:Point of care colourimetric and lateral flow LAMP assay for the detection of Haemonchus contortus in ruminant faecal samples / Rojesh Khangembam, Mariann Tóth, Nóra Vass, Marián Várady, Levente Czeglédi, Róbert Farkas, Alistair Antonopoulos
Dátum:2021
ISSN:1776-1042
Megjegyzések:In this study, we present an optimised colourimetric and a lateral flow LAMP assay for the detection of Haemonchus contortus in small ruminant faecal samples. Using a previously published LAMP primer set, we made use of commercially available colourimetric LAMP and lateral flow kits and combined this into an optimised diagnostic assay which was then tested on field faecal samples from Eastern and South-Eastern Hungary as well as a pure H. contortus egg faecal sample from Ko?sice, Slovakia. Both assays showed no conflicts in visual detection of the results. Additionally, we modified and tested several centrifuge-free DNA extraction methods and one bead-beating egg lysis DNA extraction method to develop a true point of care protocol, as the source of the starting DNA is the main ratelimiting step in farm-level molecular diagnosis. Out of the various methods trialed, promising results were obtained with the magnetic bead extraction method. Sample solutions from the Fill-FLOTAC technique were also utilised, which demonstrated that it could be efficiently adapted for field-level egg concentration to extract DNA. This proof of concept study showed that isothermal amplification technologies with a colourimetric detection or when combined with a lateral flow assay could be an important step for a true point of care molecular diagnostic assay for H. contortus.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
LAMP assay
Lateral flow
Haemonchus contortus
Point-of-Care Diagnosis
Fill-FLOTAC
Megjelenés:Parasite. - 28 (2021), p. 1-12. -
További szerzők:Tóth Mariann (1994-) (agrármérnök) Vass Nóra (1983-) (állatorvos) Várady Marián Czeglédi Levente (1977-) (agrármérnök) Farkas Róbert Antonopoulos, Alistair
Pályázati támogatás:EFOP-3.6.3-VEKOP-16-2017-00008
EFOP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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