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001-es BibID:BIBFORM087982
Első szerző:Pohóczky Krisztina
Cím:Estrogen-dependent up-regulation of TRPA1 and TRPV1 receptor proteins in the rat endometrium / Pohóczky Krisztina, Kun József, Szalontai Bálint, Szőke Éva, Sághy Éva, Payrits Maja, Kajtár Béla, Kovács Krisztina, Környei József László, Garai János, Garami András, Perkecz Anikó, Czeglédi Levente, Helyes Zsuzsanna
Dátum:2016
ISSN:0952-5041
Megjegyzések:Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors expressed predominantly in sensory nerves are activated by inflammatory stimuli and mediate inflammation and pain. Although they have been shown in the human endometrium, their regulation and function are unknown. Therefore, we investigated their estrogen- and progesterone-dependent alterations in the rat endometrium in comparison with the estrogen regulated inflammatory cytokine macrophage migration inhibitory factor (MIF). Four-week-old (sexually immature) and four-month-old (sexually mature) female rats were treated with the non-selective estrogen receptor (ER) agonist diethylstilboestrol (DES), progesterone and their combination, or ovariectomized. RT-PCR and immunohistochemistry were performed to determine mRNA and protein expression levels respectively. Channel function was investigated with ratiometric [Ca2C]i measurement in cultured primary rat endometrial cells. Both TRP receptors and MIF were detected in the endometrium at mRNA and protein levels, and their localizations were similar. Immunostaining was observed in the immature epithelium, while stromal, glandular and epithelial positivity were observed in adults. Functionally active TRP receptor proteins were shown in endometrial cells by activation-induced calcium influx. In adults, Trpa1 and Trpv1 mRNA levels were significantly up-regulated after DES treatment. TRPA1 increased after every treatment, but TRPV1 remained unchanged following the combined treatment and ovariectomy. In immature rats, DES treatment resulted in increased mRNA expression of both channels and elevated TRPV1 immunopositivity. MIF expression changed in parallel with TRPA1/TRPV1 in most cases. DES up-regulated Trpa1, Trpv1 and Mif mRNA levels in endometrial cell cultures, but 17b-oestradiol having ERa-selective potency increased only the expression of Trpv1. We provide the first evidence for TRPA1/TRPV1 expression and their estrogen-induced up-regulation in the rat endometrium in correlation with the MIF.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
transient receptor potential ankyrin 1 and vanilloid 1
macrophage migration inhibitory factor
rat endometrium
estrogen
progesterone
mRNA expression
immunohistochemistry
fluorescent calcium imaging
Megjelenés:Journal Of Molecular Endocrinology. - 56 : 2 (2016), p. 135-149. -
További szerzők:Kun József Szalontai Bálint (Pécsi Egyetem) Szőke Éva Sághy Éva Payrits Maja Kajtár Béla (1977-) (patológus) Kovács Krisztina Környei József László Garai János Garami András Perkecz Anikó Czeglédi Levente (1977-) (agrármérnök) Helyes Zsuzsanna
Pályázati támogatás:KTIA_NAP_13-2014- 0022
Egyéb
4.2.4.A/2-11/1-2012-0001
TÁMOP
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