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001-es BibID:	BIBFORM046862
Első szerző:	Penyige András (molekuláris genetikus)
Cím:	Modification of Glutamine Synthetase in Streptomyces griseus by ADP-Ribosylation and Adenylylation / Penyige, A., Kalmanczhelyi, A., Sipos, A., Ensign, J. C., Barabas, G.
Dátum:	1994
ISSN:	0006-291X
Megjegyzések:	Addition of NH+4 to Streptomyces griseus 2682 cells grown in NO?3 containing medium resulted in a rapid decline in glutamine synthetase activity due to covalent modification of the enzyme. The NH+4 promoted inactivation of the enzyme was inhibited by the ADP-ribosyltransferase inhibitor 3-methoxybenzamide. In the presence of ADP-ribosyltransferase activity the purified glutamine synthetase was also inhibited by NAD+ in a concentration-dependent manner. ADP-ribosylation of glutamine synthetase was demonstrated in vitro by showing the incorporation of labeled ADP-ribose from [?-32P]NAD+ into glutamine synthetase subunits. Beside ADP-ribosylation, adenylylation of glutamine synthetase was also shown in S. griseus since phosphodiesterase I treatment reactivated the enzyme in crude extracts of NH+4-shocked cells. Glutamine synthetase was also inhibited and modified by ATP in crude cellular extracts. These results suggest that in S. griseus 2682 ADP-ribosylation of glutamine synthetase could be an alternative modification to adenylylation to regulate glutamine synthetase activity.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Biochemical And Biophysical Research Communications. - 204 : 2 (1994), p. 598-605. -
További szerzők:	Kálmánczhelyi Attila Sipos Anikó Ensign, J. C. Barabás György (1933-) (sejtbiológus, molekuláris genetikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM004716
Első szerző:	Penyige András (molekuláris genetikus)
Cím:	Depolarization of the membrane potential by beta-lactams as a signal to induce autolysis / Penyige, A., Matko, J., Deak, E., Bodnar, A., Barabas, G.
Dátum:	2002
Megjegyzések:	The effect of beta-lactam antibiotics that are known to inhibit cell wall biosynthesis and induce cell wall autolysis on the electrophysiological state of the plasma membrane in Streptomyces griseus was studied. Addition of various beta-lactam antibiotics induced a dose- and growth-stage-dependent depolarization of the membrane potential of Streptomyces griseus. The hydrolyzed biologically inactive derivative penicilloic acid had no depolarizing effect on the membrane potential. The ionophore gramicidin D, while depolarizing the membrane potential, also induced a dose-dependent increase in cell wall lysis. These observations suggest that alteration of the transmembrane potential could be an important signal in triggering cell wall autolysis of S. griseus.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Anti-Bacterial Agents Antibiotics Autolysis biosynthesis Carbocyanines Cefotaxime Coloring Agents drug effects Drug Interactions Gramicidin Hungary Ionophores Membrane Potentials metabolism Penicillin G pharmacology Research Streptomyces Streptomyces griseus Support
Megjelenés:	Biochemical and Biophysical Research Communications. - 290 : 4 (2002), p. 1169-1175. -
További szerzők:	Matkó János (1952-) (biológus) Deák Eleonóra Dóczy-Bodnár Andrea (1970-) (biofizikus) Barabás György (1933-) (sejtbiológus, molekuláris genetikus)
Internet cím:	elektronikus változat DOI
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